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A preparation method for improving phycoerythrin yield

A technology of phycoerythrin and yield, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the difficulties of separation and purification procedures, high sugar content of large red algae, broken release of phycoerythrin Problems such as protein difficulties, to achieve simple procedures, lower production technology threshold, and easy to achieve results

Active Publication Date: 2022-04-19
NATIONAL TAIWAN OCEAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the algae of macroalgae are mostly thallus, it is difficult to break them and release phycoerythrin.
In addition, macroalgae have a high sugar content, which makes the subsequent separation and purification procedures more difficult

Method used

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  • A preparation method for improving phycoerythrin yield
  • A preparation method for improving phycoerythrin yield
  • A preparation method for improving phycoerythrin yield

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preparation example Construction

[0033] The preparation method for improving the yield of phycoerythrin provided by the present invention, its embodiment comprises:

[0034] Step 1, mixing an Acremonas, a fermentation broth containing Pseudoalteromonas thalassemia and a seawater to obtain a mixture of Acremonas broth, wherein the OD of the fermentation broth is 600 The value is 0.9~1.1, the OD of the fermentation broth 600 The value is preferably 1, wherein the mixing ratio of the Acrometa: the fermentation broth: the seawater is 2(g):1(mL):1(mL);

[0035] Step 2, fermenting the Acromycetes bacterium liquid mixture under one time condition, one temperature condition and one rotational speed condition, so that the Acrometrix algae is broken and released phycoerythrin, wherein the time condition is 6-78 hours, and the time condition It is preferably 6-36 hours, the best time condition is 24-30 hours, the temperature condition is 20-25°C, the best temperature condition is 22°C, the rotation speed condition is 1...

Embodiment 1

[0036] Embodiment one, preparation fermented bacteria liquid

[0037] After defrosting the cryopreserved Pseudoalteromonas marinum, take 100 μL of the bacterial liquid to the solid medium (Marineagar) to smear the plate to activate the strain. After culturing at 22°C for 12 hours, use 1 mL of phosphate buffered saline (Phosphate buffered saline , PBS) Rinse and inoculate Pseudoalteromonas marinum into 50 mL of liquid culture medium (Marinebroth), and carry out liquid culture at 22°C and 300 rpm for 12 hours, then use a spectrophotometer to detect the absorbance at 600 nm, When OD 600 When the value is 0.9-1.1, it can be used as the fermentation broth for the fermentation of Acrometrix algae.

Embodiment 2

[0038] Embodiment two, acrometa fermenting treatment

[0039] Take an acremonium and add it into a seawater, and then add the fermentation broth prepared by the method described in Example 1 to obtain a mixture of acremonium bacteria fluid. Ferment the Acrometrix bacterium liquid mixture under one temperature condition and one rotational speed condition, so that the Acrometrix algae is broken to release the water-soluble phycoerythrin, and the acrometrix is ​​broken at 0, 6, 12, 24, 30, 36, 48, 54, Samples were taken at 60, 72 and 78 hours for detection of phycoerythrin content. Wherein, the acromega: the seawater: the mixing ratio of the fermentation liquid is 2 (g): 1 (mL): 1 (mL); wherein the seawater is artificial seawater or natural seawater; wherein the temperature condition is 20- 25°C, the optimal temperature condition is 22°C; the rotational speed condition is 100-500rpm, the preferred rotational speed condition is 200-400rpm, and the optimum rotational speed conditi...

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Abstract

The invention provides a preparation method for increasing the output of phycoerythrin, which comprises step 1, mixing Acrometrix algae, fermented bacterial liquid containing Pseudoalteromonas thalassemia and seawater, to obtain a mixture of Acremona bacterium liquid; step 2, mixing The mixture of the Acrometrix bacterium is fermented under the conditions of one time, one temperature and one rotational speed, so that the Acrometrix is ​​crushed and released phycoerythrin; the present invention also provides a use of bacteria for crushing the Acrometrix.

Description

technical field [0001] The invention relates to a preparation method for increasing the yield of phycoerythrin, in particular to a preparation method for increasing the yield of phycoerythrin by using bacterial fermentation. Background technique [0002] Phycoerythrin (Phycoerythrin, PE) is a kind of phycobiliprotein (Phycobiliprotein), which is a photosynthetic pigment protein used by algae such as blue-green algae, red algae, and cryptopterous algae to supplement light. Due to its spectral and fluorescent properties, phycoerythrin has been widely used in applications related to fluorescent labeling, such as biological indicator reagents, chemical preparations, in vitro diagnostic labels, fluorescent detection probes, etc. In recent years, phycoerythrin has also been used in food, cosmetics and health products. [0003] However, although phycoerythrin has high industrial applicability, it has high production technology threshold and low output, which leads to high cost of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02C12N1/20C12R1/89C12R1/01
CPCC12P21/02C07K14/405C12N1/20
Inventor 李孟洲黄晋毅张富杰叶翰扬陈奕荣
Owner NATIONAL TAIWAN OCEAN UNIVERSITY
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