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Preparation method for improving phycoerythrin yield

A technology for phycoerythrin and yield, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of high sugar content in large red algae, difficulty in crushing and releasing phycoerythrin, separation and purification Problems such as difficult procedures, to achieve the effect of lowering the threshold of production technology, easy to achieve, and simple procedures

Active Publication Date: 2021-03-12
NATIONAL TAIWAN OCEAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the algae of macroalgae are mostly thallus, it is difficult to break them and release phycoerythrin.
In addition, macroalgae have a high sugar content, which makes the subsequent separation and purification procedures more difficult

Method used

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  • Preparation method for improving phycoerythrin yield
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  • Preparation method for improving phycoerythrin yield

Examples

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preparation example Construction

[0033] The preparation method for improving the yield of phycoerythrin provided by the present invention, its embodiment comprises:

[0034] Step 1, mixing an Acremonas, a fermentation broth containing Pseudoalteromonas thalassemia and a seawater to obtain a mixture of Acremonas broth, wherein the OD of the fermentation broth is 600 The value is 0.9~1.1, the OD of the fermentation broth 600 The value is preferably 1, wherein the mixing ratio of the Acrometa: the fermentation broth: the seawater is 2(g):1(mL):1(mL);

[0035] Step 2, fermenting the Acromycetes bacterium liquid mixture under one time condition, one temperature condition and one rotational speed condition, so that the Acrometrix algae is broken and released phycoerythrin, wherein the time condition is 6-78 hours, and the time condition It is preferably 6-36 hours, the best time condition is 24-30 hours, the temperature condition is 20-25°C, the best temperature condition is 22°C, the rotation speed condition is 1...

Embodiment 1

[0036] Embodiment one, preparation fermented bacteria liquid

[0037] After defrosting the cryopreserved Pseudoalteromonas marinum, take 100 μL of the bacterial liquid to the solid medium (Marineagar) to smear the plate to activate the strain. After culturing at 22°C for 12 hours, use 1 mL of phosphate buffered saline (Phosphate buffered saline Pseudoalteromonas marinum, PBS) was rinsed and inoculated into 50 mL of liquid medium (Marinebroth), and cultured in a liquid state at 22°C and 300 rpm for 12 hours, and then the absorbance at 600 nm was detected by a spectrophotometer. When OD 600 When the value is 0.9-1.1, it can be used as the fermentation broth for the fermentation of Acrometrix algae.

Embodiment 2

[0038] Embodiment two, acrometa fermenting process

[0039] Take an acremonium and add it into a seawater, and then add the fermentation broth prepared by the method described in Example 1 to obtain a mixture of acremonium broth. Ferment the Acrometrix bacterium liquid mixture under one temperature condition and one rotational speed condition, so that the Acrometrix algae is broken to release the water-soluble phycoerythrin, and it is fermented at 0, 6, 12, 24, 30, 36, 48, 54, Samples were taken at 60, 72 and 78 hours for detection of phycoerythrin content. Wherein, the Acrometrix: the seawater: the mixing ratio of the fermentation liquid is 2 (g): 1 (mL): 1 (mL); wherein the seawater is artificial seawater or natural seawater; wherein the temperature condition is 20- 25°C, the optimal temperature condition is 22°C; the rotational speed condition is 100-500rpm, the preferred rotational speed condition is 200-400rpm, and the optimum rotational speed condition is 300rpm.

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Abstract

The invention provides a preparation method for improving phycoerythrin yield, which comprises the following steps: step 1, mixing acrochaetiaceae, fermentation bacteria liquid containing pseudoalteromonas wandering and seawater to obtain a acrochaetiaceae bacteria liquid mixture; and step 2, fermenting the acrochaetiaceae bacterial liquid mixture under a time condition, a temperature condition and a rotating speed condition, so that the acrochaetiaceae is crushed to release phycoerythrin. The invention further provides an application of the bacteria for breaking the acrochaetiaceae.

Description

technical field [0001] The invention relates to a preparation method for increasing the yield of phycoerythrin, in particular to a preparation method for increasing the yield of phycoerythrin by using bacterial fermentation. Background technique [0002] Phycoerythrin (Phycoerythrin, PE) is a kind of phycobiliprotein (Phycobiliprotein), which is a photosynthetic pigment protein used by algae such as blue-green algae, red algae, and cryptopterous algae to supplement light. Due to its spectral and fluorescent properties, phycoerythrin has been widely used in applications related to fluorescent labeling, such as biological indicator reagents, chemical preparations, in vitro diagnostic labels, fluorescent detection probes, etc. In recent years, phycoerythrin has also been used in food, cosmetics and health products. [0003] However, although phycoerythrin has high industrial applicability, it has high production technology threshold and low output, which leads to high cost of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C12N1/20C12R1/89C12R1/01
CPCC12P21/02C07K14/405C12N1/20
Inventor 李孟洲黄晋毅张富杰叶翰扬陈奕荣
Owner NATIONAL TAIWAN OCEAN UNIVERSITY
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