Reagent device and secondary staining method

Abstract

The invention discloses a reagent device and a secondary staining method. A sampling nozzle in a sampling device punctures a sealing film at the top of a first container after sampling, and a sample is mixed with a first reagent in the first container to finish first staining; the sampling device and the first container are continuously pushed or rotated downwards to enable a piercing device to bein contact with and pierce a bottom sealing film, and the sample mixed solution after the first staining is mixed with a second reagent in a second container to form a sample mixed solution after thesecond staining; and the sampling device is inverted together with the first container and the second container, the sample mixed solution stained for the second time is dropped into a mixed solutionpaving device from a sample outlet nozzle through the second container, the first container, the sampling nozzle and a sample accommodating channel in sequence, and the mixed solution is flattened inthe mixed solution paving device for microscopic examination. The reagent device is simple and ingenious in structure, very convenient to perform efficient secondary staining, simple to operate, goodin cell staining effect, obvious in stained cell characteristics and suitable for cell analysis in a bright field of view.

Description

technical field [0001] The present application belongs to the field of cell staining technology and devices, in particular to the field of cell staining technology and devices before detection of cells in body fluids or secretions, and in particular to a staining device, staining reagent and staining method for cell staining in body fluids or secretions. Background technique [0002] In the cell detection of biological body fluids or secretions, in order to distinguish the types, shapes and states of cells in body fluids or secretions, cell staining techniques are usually used to distinguish cells of different types, shapes, and states. [0003] In the conventional cell staining technology for bright field observation and detection, the sample to be tested is usually smeared on a transparent carrier such as a glass slide. After the dyeing is evenly completed, the main body of the dye solution and buffer solution that affect the observation is washed away, and the dyed sample...

AI Technical Summary

Problems solved by technology

[0007] It can be seen from the above steps that the dyeing steps in the prior art are extremely cumbersome and time-consuming, and the operation of each step requires well-trained professional operators to produce qualified observation samples

The staining efficiency in the prior art is extremely low, and it is impossible to stain sample cells in large quantities

Images