Deep-sea roseobacter and application of deep-sea roseobacter in induction of attachment of mytilus coruscus

A thick-shelled mussel and deep-sea technology is applied to the deep-sea rose bacterium and its application in inducing the thick-shelled mussels to attach, and can solve the problem that natural seedlings cannot satisfy the thick-shelled mussels and the like.

Active Publication Date: 2021-03-30
SHANGHAI OCEAN UNIV
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Problems solved by technology

However, with the over-exploitation of fishery resources, natural seedlings can no longer satisfy the development of the mussel culture industry. It is particularly urgent to solve the problem of seedlings and explore bioengineering technologies such as the use of bacteria to form biofilms to induce the attachment of mussels.

Method used

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  • Deep-sea roseobacter and application of deep-sea roseobacter in induction of attachment of mytilus coruscus
  • Deep-sea roseobacter and application of deep-sea roseobacter in induction of attachment of mytilus coruscus
  • Deep-sea roseobacter and application of deep-sea roseobacter in induction of attachment of mytilus coruscus

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Embodiment 1

[0021] The induction experiment of roseobacterium deep-sea Roseovarius nubinhibens G213-d on the attachment of thick-shelled mussel juveniles, the specific steps are as follows: the pure roseobacterium deep-sea Roseovarius nubinhibens G213-d strain was placed in seawater 2216E liquid medium for 48 hours, centrifuged Get the bacterial precipitate, wash the bacteria with sterilized seawater and make a suspension, repeat 3 times, the obtained bacterial solution is fixed to 50mL, after staining with acridine orange, randomly select 10 points under a fluorescent microscope with a lens of 100 times for counting , to determine the total density of the bacterial solution to be 2×10 9 cells / mL. Put a sterilized glass slide in each petri dish, and add corresponding sterilized seawater and bacterial solution, so that the initial density of bacteria is 1.0×10 6 cells / mL, 1.0×10 7 cells / mL, 1.0×10 8 cells / mL and 5×10 8 cells / mL, cultured at 18°C ​​and protected from light for 48 hours ...

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Abstract

The invention discloses deep-sea roseobacter and application of the deep-sea roseobacter in induction of attachment of mytilus coruscus, and belongs to the technical field of marine microorganisms. The deep-sea roseobacter is named as deep-sea roseobacter Roseovarius nubinhibens G213-d, is preserved in China Center for Type Culture Collection (CCTCC), has a preservation number of CCTCC NO: M 2019807, has a 16S rRNA gene as shown in SEQ ID NO: 1, and is derived from ocean sediments in sea areas near South China Sea cold spring at the altitude of -1151.16 meters. According to the invention, Roseudomonas nubinhibens G213-d is obtained through separation, purification and identification, and meanwhile, it is verified that a biofilm formed by the Roseobacterium nubinhibens G213-d has activity of inducing attachment of mytilus coruscus, so that a new technical approach is provided for seedlings and induction of attachment of mytilus coruscus by using the biofilm formed by the bacteria.

Description

technical field [0001] The invention relates to the technical field of marine microorganisms, in particular to roseobacter deep sea and its application in inducing the attachment of sclerosus mussels. Background technique [0002] Thick-shelled mussels (Mytilus coruscus), belonging to the phylum Mollusca, class Bivalvia, order Mytilodia, family Mytilidae, are important marine shellfish species in my country, distributed in Along the Yellow Sea, Bohai Sea and East China Sea. There are two kinds of lifestyles of the thick-shelled mussel, including planktonic life and attached life. In the later stage of the planktonic life stage of the thick-shelled mussel, the eye-spot larvae will attach and metamorphose into juveniles, and finally grow into adults. During the growth of juveniles into adults, once the environment changes, juvenile mussels can cut off the silk and re-select the appropriate attachment base for reattachment. [0003] As a marine shellfish cultured species with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01K61/54C12R1/01
CPCA01K61/54Y02A40/81
Inventor 杨金龙彭莉华梁箫魏玉利方家松
Owner SHANGHAI OCEAN UNIV
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