Deep sea halomonas and application thereof in induction of mytilus coruscus adhesion

A technology of halophilic bacteria and thick-shelled mussels, applied in the field of marine microorganisms, can solve the problem that natural seedlings cannot meet the needs of thick-shelled mussels

Pending Publication Date: 2021-03-30
SHANGHAI OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, with the over-exploitation of fishery resources, natural seedlings can no longer satisfy the development of the mussel culture industry. It is particularly urgent to solve the problem of seedlings and explore bioengineering technologies such as the use of bacteria to form biofilms to induce the attachment of mussels.

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  • Deep sea halomonas and application thereof in induction of mytilus coruscus adhesion
  • Deep sea halomonas and application thereof in induction of mytilus coruscus adhesion
  • Deep sea halomonas and application thereof in induction of mytilus coruscus adhesion

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Embodiment 1

[0021] The induction experiment of deep-sea Halomonassp.c7200b on the attachment of thick-shelled mussel juveniles, the specific steps are as follows: the pure-bred deep-sea Halomonassp.c7200b strain was placed in seawater 2216E liquid medium and expanded for 48 hours , centrifuge to get the bacterial precipitate, wash the bacteria with sterilized seawater and make a suspension, repeat 3 times, the obtained bacterial solution is fixed to 50mL, after staining with acridine orange, randomly select 10 points under a fluorescent microscope with a lens of 1000 times After counting and measuring the bacterial density, add the bacterial stock solution into a sterile glass petri dish filled with sterilized glass slides, and finally add sterilized seawater to make the volume to 20mL, so that the initial density of bacteria is 1.0×10 6 cells / mL, 1.0×10 7 cells / mL, 1.0×10 8 cells / mL and 5×10 8 cells / mL, set 12 parallel. Cultured at 18°C ​​and protected from light for 48 hours to form ...

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Abstract

The invention discloses deep sea halomonas and application thereof in induction of mytilus coruscus adhesion, and belongs to the technical field of marine microorganisms. The deep sea halomonas is named as deep sea halomonas Halomonassp.c7200b, and is preserved in China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 2019803, and a 16S rRNA gene of the deep sea Halomonassp.c7200b is shown as SEQ ID NO: 1 and is derived from a south pacific maca gully water area at the altitude of -7200m. The deep sea halomonas Halomonassp.c7200b is obtained through separation, purification and identification, meanwhile, it is verified that a biofilm formed by the deep sea halomonas Halomonassp.c7200b has the activity of inducing attachment of mytilus coruscus, and a new technical way is provided for seedlings and induction of attachment of the mytilus coruscus through the biofilm formed by the bacteria.

Description

technical field [0001] The invention relates to the technical field of marine microorganisms, in particular to deep-sea halophilic bacterium and its application in inducing the attachment of thick-shelled mussels. Background technique [0002] Thick-shelled mussels (Mytilus coruscus), belonging to the phylum Mollusca, class Bivalvia, order Mytilodia, family Mytilidae, are important marine shellfish species in my country, distributed in Along the Yellow Sea, Bohai Sea and East China Sea. There are two kinds of lifestyles of the thick-shelled mussel, including planktonic life and attached life. In the later stage of the planktonic life stage of the thick-shelled mussel, the eye-spot larvae will attach and metamorphose into juveniles, and eventually grow into adults. During the growth of juveniles into adults, once the environment changes, juvenile mussels can cut off the silk and re-select the appropriate attachment base for reattachment. [0003] As a marine shellfish cultur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01K61/54C12R1/01
CPCA01K61/54Y02A40/81
Inventor 魏玉利方家松王珂曹军伟程玉兰杨金龙梁箫
Owner SHANGHAI OCEAN UNIV
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