Efficient co-aggregation aerobic denitrification combined bacteria agent and preparation method and application thereof

A technology of nitrifying combined bacterial agents and combined bacterial agents, applied in the direction of biochemical equipment and methods, chemical instruments and methods, aerobic process treatment, etc. Aggregation of the optimal combination of bacterial agents, no evaluation of co-aggregation ability, etc., to improve the efficiency of inorganic nitrogen removal, achieve rapid start-up, and promote the effect of bacterial abundance

Inactive Publication Date: 2021-04-09
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on strains capable of self-aggregation and aerobic denitrification, and there is no evaluation of their co-aggregation ability, and the optimal combination of bacterial agents for co-aggregation has not been obtained. Evaluate

Method used

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  • Efficient co-aggregation aerobic denitrification combined bacteria agent and preparation method and application thereof
  • Efficient co-aggregation aerobic denitrification combined bacteria agent and preparation method and application thereof
  • Efficient co-aggregation aerobic denitrification combined bacteria agent and preparation method and application thereof

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Embodiment 1

[0048] An application of a high-efficiency co-aggregation aerobic denitrification combined bacterial agent comprises the following steps:

[0049] (1) Get bacterial strain Pseudomonas mendoza IHB602, Methylobacterium DC-1 and Pseudomonas stutzeri IHB618;

[0050] (2) Place the strain in DTM to grow, take regular samples at intervals of 6 hours, and measure OD 600 value, and based on time and OD 600 Values ​​are plotted against growth curves during late logarithmic growth stages (e.g. figure 1 ) Harvest the bacterial liquid, centrifuge, remove the supernatant, and obtain concentrated bacterial strains;

[0051] (3) Resuspend the concentrated strain in co-aggregation buffer (1mM CaCl 2 , 150mM NaCl, 10mM Tris, 1mM MgCl 2 , pH 7.0) to obtain a suspension A containing the strain;

[0052] (4) Adjust the absorbance value of suspension A to OD 600 =1, to obtain suspension B;

[0053] (5) according to the method of step (1)-(3), respectively prepare the suspension of Pseudomon...

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Abstract

The invention belongs to the technical field of wastewater treatment, and particularly relates to an efficient co-aggregation aerobic denitrification combined bacteria agent and a preparation method and application thereof. The combined bacteria agent is composed of pseudomonas mendocina IHB602, methylobacterium DC-1 and pseudomonas stutzeri IHB618, the combined bacteria agent can significantly improve the inorganic nitrogen removal efficiency, and the preparation method and the application process of the combined bacteria agent are simple in operation and low in energy consumption and cost.

Description

technical field [0001] The invention belongs to the technical field of wastewater treatment, and in particular relates to a high-efficiency co-aggregation aerobic denitrification combined bacterial agent and its preparation method and application. Background technique [0002] Nitrogen removal efficiency is one of the most important issues in wastewater treatment. Since conventional biological denitrification involves two processes, aerobic nitrification and anaerobic denitrification, the two types of flora have different requirements for dissolved oxygen and organic carbon, and these two processes are always performed in different reactors or at different times in one The sequential reactors are operated separately, resulting in complex process operation, high energy consumption and high cost. Aerobic denitrification technology provides a method to overcome the above shortcomings of conventional denitrification technology, converting nitrate to nitrogen gas under aerobic c...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/38C12R1/01C02F101/16
CPCC12N1/20C02F3/34C02F3/02C02F2101/16Y02W10/10
Inventor洪培疏义林吴海龙汪建中
OwnerANHUI NORMAL UNIV