MiRNA marker related to endometrial receptivity auxiliary diagnosis and application of miRNA marker

An endometrium and auxiliary diagnosis technology, which is applied in the fields of genetic engineering and reproductive medicine, can solve problems such as time-consuming, maternal damage, tolerance error, etc., and achieve the effect of avoiding damage and preventing interference

Active Publication Date: 2021-04-16
成都西囡妇科医院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current routine detection method is to perform biopsy on the endometrium first. Because the biopsy is an invasive operation, it is necessary to perform embryo transfer in the next IVF-ET cycle, and there are errors in the tolerance of different cycles between the two cycles. , while consuming a lot of time, the damage to the mother body is also irreversible

Method used

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  • MiRNA marker related to endometrial receptivity auxiliary diagnosis and application of miRNA marker
  • MiRNA marker related to endometrial receptivity auxiliary diagnosis and application of miRNA marker
  • MiRNA marker related to endometrial receptivity auxiliary diagnosis and application of miRNA marker

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Embodiment 1

[0043] A miRNA marker for auxiliary diagnosis of endometrial receptivity, including hsa-miR-16-2-3p, hsa-miR-92b-5p, hsa-miR-130a-3p, has-miR-9024-3p miRNA and its primers.

[0044] In order to further realize the purpose of fast and accurate, the primer of described miRNA comprises the primer of hsa-miR-16-2-3p as shown in SEQ ID No.1, the primer of hsa-miR-92b-5p is as shown in SEQ ID No.2 As shown, the primers of hsa-miR-130a-3p are shown in SEQ ID No.3, and the primers of has-miR-9024-3p are shown in SEQ ID No.4; by limiting the marker primers of miRNA, specific screening The last four primers are the detection basis, and the primers for compound detection can be constructed, so that a large number of miRNA sequences to be detected can be amplified, so that the detection data sample is large enough, so as to achieve the purpose of fast and accurate.

[0045] In order to further realize the accurate purpose, described miRNA comprises the marker sequence of hsa-miR-16-2-3p ...

Embodiment 2

[0066] By finally determining the content data of different miRNAs in different periods, the ROC curve of the combination of four miRNA markers was constructed by multiple regression analysis. The four miRNAs (hsa-miR-16-2-3p, hsa-miR-92b-5p, hsa -miR-130a-3p, has-miR-9024-3p) combination produces the largest area under the ROC curve (AUC) as Figure 5 As shown, the AUC value of the curve in the figure is 0.857, the threshold value is 2.51, the sensitivity is 83.33, and the specificity is 85.71. At the same time, the curve is deduced to obtain the prediction index:

[0067] Prediction index=CT value of 0.4167*(miR-16-2-3p)+CT value of 0.1651*(miR-92b-5p)+CT value of 0.3273*(miR-130a-3p)+0.0909*(miR-9025 -3p) CT value.

[0068] Predict the receptivity of the entire sample through the predictive index, test with the sample on the day of LH=5, randomly select 200 samples, and compare the actual test data, the accuracy rate can reach 85%, which shows the superiority of the presen...

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Abstract

The invention discloses a miRNA (micro ribonucleic acid) marker for auxiliary diagnosis of endometrial receptivity. The miRNA marker comprises miRNAs of hsa-miR-16-2-3p, hsa-miR-92b-5p, hsa-miR-130a-3p and has-miR-9024-3p, and primers of the miRNAs, the invention also provides an application of the miRNA marker for auxiliary diagnosis of endometrial receptivity, which is characterized by comprising the following steps: S1, collecting female parent blood, and preparing a supernatant; s2, performing treatment in a centrifugal column filter tube, and performing graded filtration to prepare a miRNA crude product; s3, preparing a miRNA sample; s4, synthesizing cDNA (complementary deoxyribonucleic acid) from the obtained miRNA by utilizing the kit, and performing a PCR (polymerase chain reaction) amplification system and procedure to obtain content data of the miRNA; s5, constructing a CT content curve, and determining different miRNA contents in different periods; the method has the beneficial effects that miRNA and primers thereof are limited, and four primers after specific screening are taken as detection bases, so that the endometrial receptivity period can be quickly and accurately recognized.

Description

technical field [0001] The invention relates to the fields of genetic engineering and reproductive medicine, in particular to a miRNA marker for auxiliary diagnosis of endometrial receptivity and its application. Background technique [0002] At present, the incidence of infertility in China is about 15-20%, and the number of infertile patients is increasing at a rate of hundreds of thousands every year. Factors such as delayed marriage and childbearing age, accumulation of work pressure, and changes in lifestyle have directly or indirectly reduced people's fertility. With the birth of the world's first test-tube baby, in vitro fertilization-embryo transfer (IVF-ET) has become an effective way for infertile women to get pregnant as soon as possible. Although the development and technological innovation of assisted reproductive technology have enabled most infertility patients to obtain high-quality embryos through in vitro embryo culture technology and then become pregnant,...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6851
Inventor 沙郁林贾研刘敬叶红霞孟祥黔王静曲婷东亚君邱竹黄燕李鹏昊闫晓苗张素娟李晓开郭燕华冯玉婷姜瑶钟影
Owner 成都西囡妇科医院有限公司
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