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Method for cultivating low-potassium-tolerant rice

A low-potassium-tolerant rice technology, applied in the field of genetic engineering, can solve the problems of lack of potassium-tolerant rice varieties and low low-potassium tolerance

Pending Publication Date: 2021-04-30
HUNAN NORMAL UNIVERSITY
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Problems solved by technology

[0004] The application provides a method for cultivating low-potassium-tolerant rice to solve the problems of low-potassium-tolerant rice and the lack of potassium-tolerant rice varieties

Method used

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  • Method for cultivating low-potassium-tolerant rice
  • Method for cultivating low-potassium-tolerant rice
  • Method for cultivating low-potassium-tolerant rice

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Embodiment Construction

[0026] The embodiments will be described in detail hereinafter, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, the same numerals in different drawings refer to the same or similar elements unless otherwise indicated. The implementations described in the following examples do not represent all implementations consistent with this application. These are merely examples of systems and methods consistent with aspects of the present application as recited in the claims.

[0027] see figure 1 , is a flow chart of a method for cultivating low-potassium tolerant rice. The application provides a method for cultivating low-potassium tolerant rice, specifically comprising the following steps:

[0028] The rice OsHAK7 gene was cloned; the pHB-OsHAK7 overexpression recombinant plasmid was constructed; OsHAK7 overexpression positive plants were obtained; low potassium tolerant rice was obtained.

[0029...

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Abstract

The invention provides a method for cultivating low-potassium-tolerant rice. The method specifically comprises the following steps: cloning a rice OsHAK7 gene; constructing a pHB-OsHAK7 overexpression recombinant plasmid; obtaining an OsHAK7 overexpression positive plant; and obtaining low potassium tolerant rice. The invention provides an application of an OsHAK7 gene in improving the low potassium tolerance of rice. The OsHAK7 gene is responsible for absorbing K+ from soil in rice. The knockout mutant of the OsHAK7 gene is sensitive to low potassium, the K+ absorption of rice is significantly reduced, and the potassium ion content in the plant is significantly reduced. The K+ absorption capacity of an OsHAK7 gene overexpression plant is obviously enhanced, the potassium ion content in the plant is obviously increased, and the low-potassium tolerance capacity of the plant is obviously enhanced. According to the invention, the low potassium tolerance of rice is improved, and a new gene resource is provided for cultivating a new rice variety suitable for potassium-deficient soil.

Description

technical field [0001] The application relates to the technical field of genetic engineering, in particular to a method for cultivating low-potassium tolerant rice. Background technique [0002] Potassium ions are very abundant divalent cations in plant cells, and play an important role in a series of physiological and biochemical processes such as photosynthesis, regulation of cell turgor pressure and osmotic potential. Potassium is an essential mineral element for plant growth and development. Potassium deficiency will seriously affect the yield and quality of crops. [0003] The physiological process of rice absorbing potassium ions from the soil, transporting potassium ions to various parts of the ground through xylem vessels, and redistributing potassium ions is responsible for the transport proteins on the cell membrane, and various potassium transport proteins and channel proteins on the cell membrane are controlled by transcription factors and The regulation of regu...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/84A01H4/00A01H5/00A01H6/46
CPCC07K14/415C12N15/8271A01H4/005A01H4/008
Inventor 毛丹丹陈良碧孙志威
Owner HUNAN NORMAL UNIVERSITY
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