Remediation method for heavy metal contaminated soil
A technology of contaminated soil and remediation method, which is applied in the restoration of contaminated soil, land preparation methods, agricultural machinery and tools, etc., and can solve the problems of easily destructive soil microbial structure, single heavy metal remediation, and huge projects
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Embodiment 1
[0027] Preparation of immobilized microorganisms:
[0028] (1) after the palygorskite is pulverized to 90 orders, it is for subsequent use;
[0029] (2) After activation and purification of Pseudomonas on lysate broth (Luria-Bertani, LB) solid medium, inoculate in LB liquid medium and shake culture at 37°C and 150rpm until OD 600 0.7, then add 90-mesh palygorskite according to the dosage ratio of 1L:25g, shake and immobilize at a temperature of 37°C and a rotation speed of 100rpm for 12h, then filter, and freeze-dry at -20°C for 5h to obtain immobilized microorganisms;
[0030] Described LB liquid culture medium formula is:
[0031] Tryptone 10g, yeast extract 5g, NaCl 10g, deionized water 1000mL;
[0032] The LB solid medium formula is:
[0033] Tryptone 10g, yeast extract 5g, NaCl 10g, agar 17g, deionized water 1000 mL.
Embodiment 2
[0035] This embodiment provides a method for repairing heavy metal-contaminated soil, comprising the following steps:
[0036] S1, preparation of bioremediation agent: mix the immobilized microorganisms prepared in Example 1 with the lactic acid bacteria powder according to the mass ratio of 15:5 to obtain the bioremediation agent;
[0037] The brand name of the lactic acid bacteria powder is Xinlaiwang III-5000, including Lactobacillus acidophilus, Lactobacillus plantarum and Enterococcus faecium, the total number of lactic acid bacteria is ≥5×10 11 cfu / g, purchased from Xinlaiwang Biotechnology Co., Ltd.;
[0038] S2, add 90g of the bioremediation agent prepared in S1 to every 1 square meter of heavy metal-contaminated soil, plow the soil, and let it stand for 20 days;
[0039] S3, then add passivation agent to the soil after S2 remediation, the addition amount is 35g / m 3 , and then plow the soil again, and let it stand still for 25 days;
[0040] The passivating agent is...
Embodiment 3
[0045] This embodiment provides a method for repairing heavy metal-contaminated soil, comprising the following steps:
[0046] S1, preparation of bioremediation agent: mix the immobilized microorganisms prepared in Example 1 and lactic acid bacteria powder uniformly according to the mass ratio of 10:5 to obtain bioremediation agent;
[0047] The brand name of the lactic acid bacteria powder is Xinlaiwang III-5000, including Lactobacillus acidophilus, Lactobacillus plantarum and Enterococcus faecium, the total number of lactic acid bacteria is ≥5×10 11 cfu / g, purchased from Xinlaiwang Biotechnology Co., Ltd.;
[0048]S2, add 80g of the bioremediation agent prepared in S1 to every 1 square meter of heavy metal-contaminated soil, plow the soil, and let it stand for 10 days;
[0049] S3, then add passivation agent to the soil after S2 remediation, the addition amount is 30g / m 3 , and then plow the soil again, and let it stand still for 20 days;
[0050] The passivating agent is...
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