Method for obtaining large-batch high-quality explants by castanopsis hystrix branch bending and germination promotion technology and application thereof

An explant and large-scale technology, applied in the fields of botanical equipment and methods, applications, horticultural methods, etc., can solve the problems of low multiplication ratio, plant death, and high cost and cost

Active Publication Date: 2021-06-04
GUANGDONG ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the multiplication factor of red cone is low in tissue culture, and too many subcultures will reduce the growth vigor and survival rate.
At present, the methods of obtaining explants in the non-growth period mainly include cutting piles to preserve germination and circumcision to promote germination. Among them, cutting piles to preserve germination requires cutting down excellent trees, which is destructive and irreversible, and the cost is high; ring cutting often occurs to promote germination Post-germination is more difficult, and the plant will die, causing irreparable losses
It can be seen that the above method is directly used for red cones, and the gain outweighs the gain. Therefore, it is urgent to find a way to effectively obtain a large number of explants.

Method used

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  • Method for obtaining large-batch high-quality explants by castanopsis hystrix branch bending and germination promotion technology and application thereof
  • Method for obtaining large-batch high-quality explants by castanopsis hystrix branch bending and germination promotion technology and application thereof
  • Method for obtaining large-batch high-quality explants by castanopsis hystrix branch bending and germination promotion technology and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058]A kind of method of red cone bending branch germination promoting technology to obtain large quantities of high-quality explants, such as figure 1 shown, including the following steps:

[0059] S1. Substrate treatment: the substrates are yellow core soil, peat soil and mixed soil (the mixed soil is obtained by mixing yellow core soil and peat soil at a mass ratio of 1:1), and the day before planting, use high manganese with a concentration of 0.5% (w / v) Disinfect with potassium acid solution, cover with film for one day, remove the film before planting, and pour water 2 to 3 times to thoroughly water the substrate;

[0060] S2. Pruning: Carry out truncation treatment to the trunk branches of the red cone nutrient bag seedlings, cut off some side branches on the trunk of the red cone branches, and leave 2 to 3 side branches near the top;

[0061] S3. Field planting: Prune in the hole (30cm * 30cm * 20cm) with the branch that has 2~3 near top lateral branches that pruning...

Embodiment 2

[0070] The operation steps of this embodiment are basically the same as that of Example 1. The culture substrate used in the S3 planting step is mixed soil (the mixed soil is obtained by mixing yellow core soil and peat soil at a mass ratio of 1:1), the difference is that the steps of this embodiment In S2, the following 5 pruning methods were used for the pruning operation of the red cone branches, and the unpruned one was used as the control (CK), and 5 groups of repetitions were set for each method, with 4 plants in each group.

[0071] The 5 pruning methods are:

[0072] B1: same as embodiment 1, carry out truncation treatment, cut off some side branches on the trunk of the red cone branch, stay 2~3 side branches near the top;

[0073] B2: Carry out top truncation treatment and keep all side branches;

[0074] B3: No truncation treatment, cut off part of the side branches on the main trunk of the red cone branch, leaving 2 to 3 side branches near the top;

[0075] B4: Cu...

Embodiment 3

[0082] The operation steps of this embodiment are basically the same as that of Example 1. The culture substrate used in the S3 planting step is mixed soil (the mixed soil is obtained by mixing yellow core soil and peat soil at a mass ratio of 1:1), the difference is that the steps of this embodiment In S4, the following three bending methods are adopted:

[0083] A1: The trunk branches are bent at 90-120° and the branches near the top form a platform;

[0084] A2: The main branch is bent 90-120° and the branch near the top is depressed and not higher than the bent part of the branch;

[0085] A3: The trunk branches are bent 90-120° and the branches near the top are depressed but higher than the bent part of the branches.

[0086] The results are shown in Table 3 and Figure 4 shown.

[0087] Table 3 The influence of bent branches on germination

[0088]

[0089] It was found that the A2 method had a good germination-promoting effect, and the number of germinations was ...

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Abstract

The invention discloses a method for obtaining large-batch high-quality explants by a castanopsis hystrix branch bending and germination promoting technology and application thereof. The method comprises the steps of matrix treatment, pruning treatment, field planting, branch bending, soil covering, germination management and the like. According to the method, a branch bending and germination promoting mode suitable for castanopsis hystrix is established through experimental research on a bent branch culture medium, a branch pruning method before branch bending, a branch bending method and the like, the number and quality of the castanopsis hystrix explants can be remarkably improved, the problem that the number of high-quality castanopsis hystrix explants is insufficient can be effectively solved, and feasible theoretical basis and technical support can be provided for preparation of rejuvenized explants through vegetative propagation.

Description

technical field [0001] The invention belongs to the technical field of forest tree seed breeding, and in particular relates to a method for obtaining large quantities of high-quality explants through the technology of promoting germination of red cones and bent branches and the application thereof. Background technique [0002] Red cone (Castanopsis hystrix) is an evergreen tree of the Fagaceae Castanopsis genus. It is an important native broad-leaved high-quality precious timber and multi-purpose tree species in South China. It is mainly distributed in Guangdong, Guangxi, Hainan, Yunnan and other places in my country. Red cone has the characteristics of fast growth, wide adaptability, and high benefit. Its trunk is straight, hard, red, strong corrosion resistance, no cracking, no deformation, and easy to process. It can be used for construction, shipbuilding, high-end furniture, and wooden floors. , military supplies, sports equipment, etc. Red cone is more shade tolerant ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01G24/10A01G24/28A01G7/06
CPCA01G17/005A01G24/10A01G24/28A01G7/06
Inventor 张卫华潘文徐放陈新宇朱报著杨会肖
Owner GUANGDONG ACAD OF FORESTRY
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