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Application of chondroitin sulfate in preparation of medicine for preventing and treating intervertebral disc degeneration

A chondroitin sulfate and intervertebral disc technology, applied in the field of biomedicine, can solve problems such as no application of chondroitin sulfate reported in literature, and achieve the effects of preventing and treating intervertebral disc degeneration, inhibiting the reduction of intervertebral space height, and relieving intervertebral disc degeneration.

Pending Publication Date: 2021-06-11
SHANGHAI CHANGZHENG HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no literature report on the application of chondroitin sulfate in the treatment of IDD

Method used

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  • Application of chondroitin sulfate in preparation of medicine for preventing and treating intervertebral disc degeneration
  • Application of chondroitin sulfate in preparation of medicine for preventing and treating intervertebral disc degeneration
  • Application of chondroitin sulfate in preparation of medicine for preventing and treating intervertebral disc degeneration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Chondroitin sulfate and its subtypes can increase the water content of the degenerated intervertebral disc of New Zealand white rabbits and reduce the height of the intervertebral space

[0029] experimental method:

[0030] A 3-month-old 3kg male New Zealand rabbit was selected for the experiment, and the rabbits were divided into control group (sham operation group), IDD group (acupuncture model group), IDD+CS group (acupuncture model and injection of CS group), IDD+ CSA group (acupuncture model and injection of CSA group), IDD+CSC group (acupuncture model and injection of CSC group), a total of 5 groups.

[0031] The acupuncture intervertebral disc model is a commonly used animal model of IDD. The rabbits were anesthetized by injecting pentobarbital into the ear vein through lateral approach, and the injection volume of 2% pentobarbital was 1ml / kg. After the rabbit was anesthetized, the dorsal muscles were separated laterally, exposed to the front edge o...

Embodiment 2

[0040] Example 2: Chondroitin sulfate and its subtypes can inhibit the degradation of extracellular matrix of human nucleus pulposus cells

[0041] experimental method:

[0042] The intervertebral disc specimens of patients who underwent posterior lumbar decompression, bone grafting, fusion and internal fixation in the Department of Orthopedics, Shanghai Changzheng Hospital were collected from January 2020 to June 2020. The patient was informed before the operation, and the consent of the patient was obtained, and the hospital ethics review was passed.

[0043] Extract primary nucleus pulposus cells: Use ophthalmic scissors and ophthalmic forceps to cut the picked nucleus pulposus tissue, cut into smaller tissue pieces, digest with 0.25% trypsin digestion solution, centrifuge and use 0.2% II The digestion solution of type collagenase was centrifuged and cultivated under the conditions of 37 degrees centigrade and 5% CO2. When the nucleus pulposus primary cells proliferated t...

Embodiment 3

[0048] Example 3: Chondroitin sulfate and its subtypes inhibit nucleus pulposus cell apoptosis

[0049] experimental method:

[0050] The Cell Counting Kit-8 (CCK-8) assay is an important experimental method for detecting cell proliferation and toxicity analysis, and its method is simple and reliable. The experimental principle of CCK-8 is based on the dehydrogenase activity in living cells, and WST-8 in CCK-8 can undergo a redox reaction with the dehydrogenase in living cells. Redox reactions can produce highly water-soluble yellow formazan products, and the amount of formazan produced is proportional to the number of living cells. Therefore, through this ratio, the number of living cells can be calculated and the ability of cell proliferation can be analyzed.

[0051] The nucleus pulposus cells in a 10cm dish were spread in a 6-well plate. After the cells adhered to the wall, the nucleus pulposus cells were intervened with 20ng / ml IL-1β for 48 hours, and then CS, CSA, and ...

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Abstract

The invention provides application of chondroitin sulfate in preparation of a medicine for preventing and treating intervertebral disc degeneration and a medicine composition for preventing and treating intervertebral disc degeneration. The water content of the intervertebral disc can be obviously increased and / or the height reduction of intervertebral space is inhibited, so that the intervertebral disc degeneration is prevented and treated. The chondroitin sulfate is especially suitable for intervertebral disc protrusion patients who do not reach surgical indications, intervertebral disc protrusion patients who remove protruding intervertebral discs by adopting an orifice mirror technology and intervertebral disc protrusion patients who remove protruding intervertebral discs by adopting pure nucleus pulposus extraction, and is beneficial to relieving intervertebral disc degeneration, relieving lumbago and backache caused by intervertebral disc protrusion, and can promote the recovery of residual nucleus pulposus after foramen endoscope and pure nucleus pulposus removal.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of chondroitin sulfate in preparing medicine for treating intervertebral disc degeneration. Background technique [0002] Intervertebral disc degeneration (IDD) is an important pathological factor causing neck, shoulder and back pain, and plays an important role in the major diseases affecting people's health in the world today. IDD has caused a significant economic burden and medical expenses to the society, and brought a heavy burden to the people's medical expenditure and the national medical finance. According to statistics, in the United States alone, the economic loss caused by IDD is as high as 90 billion U.S. dollars (see literature: Li Y, Samartzis D, Campbell DD, et al. Two subtypes of intervertebral discdegeneration distinguished by large-scale population-based study. Spine J. 2016 Sep;16(9):1079-89.). IDD is not only harmful, but also has a hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/737A61P19/08
CPCA61K31/737A61K9/0019A61P19/08
Inventor 胡博吴卉乔王云浩臧法智徐辰陈华江曹鹏袁文
Owner SHANGHAI CHANGZHENG HOSPITAL
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