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A kind of method for separating and preparing 1,2,3,4,6-O-pentagalloglucose from almond fruit

A technology for pentagalloyl glucose and saccharin fruit, which is applied in chemical instruments and methods, esterification saccharide, preparation of sugar derivatives, etc., to achieve the effects of being suitable for large-scale production, efficient utilization, and broad economic prospects

Active Publication Date: 2022-07-29
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report of 1,2,3,4,6-O-pentagalloyl glucose found in Conghua Xiangguo

Method used

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  • A kind of method for separating and preparing 1,2,3,4,6-O-pentagalloglucose from almond fruit
  • A kind of method for separating and preparing 1,2,3,4,6-O-pentagalloglucose from almond fruit
  • A kind of method for separating and preparing 1,2,3,4,6-O-pentagalloglucose from almond fruit

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Pulverization: The natural air-dried raw materials of the fragrant fruit are pulverized with a plant pulverizer and passed through a Φ2mm sieve plate.

[0032] (2) Extraction: place 1.5kg of the crushed fragrant fruit in the extraction tank, add 15L of water, heat, control the temperature at 80°C, and the extraction time is 90min, extract 3 times, filter to remove slag, and combine the filtrates.

[0033] (3) Concentration: the extract was concentrated, and vacuum freeze-dried to obtain 578 g of powdered fragrant fruit extract.

[0034] (4) Adsorption: take 32g of Fructus fragrans extract, add 468mL of hot water to dissolve, cool to room temperature and then put on a D101 macroporous adsorption resin column for adsorption.

[0035] (5) Elution: Elution was carried out with ethanol solution with volume percentage concentration of 0, 20%, 40%, 60%, 80%, and 100% in sequence, and each eluent was used in an amount of 450 mL, and each elution fraction was collected.

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Embodiment 2

[0039] (1) Adsorption: Take 20 g of Fructus fragrans extract, add 380 mL of hot water to dissolve, and after cooling, apply it to HP-20 resin column for adsorption.

[0040](2) Elution: Elution was carried out with ethanol solutions with volume percentage concentrations of 0, 20%, 40%, 60%, 80%, and 100% in sequence, and the amount of each eluent was 500 mL, and the eluent was collected.

[0041] (3) Evaporation: the 100% ethanol elution part was evaporated under reduced pressure at a temperature of 40°C to 45°C for 1 hour, ethanol was recovered, and the aqueous solution was supplemented to a volume of about 20 mL.

[0042] (4) Drying: The aqueous solution was freeze-dried to obtain 0.15 g of a light yellow product, and the purity of 1,2,3,4,6-O-pentagalloglucose was 98.2% as determined by HPLC.

Embodiment 3

[0044] (1) Adsorption: Take 20g of Fructus fragrans extract, add 400mL of hot water to dissolve, and after cooling, go to D101 resin column for adsorption.

[0045] (2) Elution: Elute with methanol solutions with volume percentage concentrations of 0, 20%, 40%, 60%, 80%, and 100% successively, and collect the eluate with an amount of 500 mL of each eluent.

[0046] (3) Evaporation: the 100% methanol eluted part was evaporated under reduced pressure at a temperature of 40°C to 45°C for 1 h, methanol was recovered, and the aqueous solution was supplemented to a volume of about 20 mL.

[0047] (4) Drying: The aqueous solution was freeze-dried to obtain 0.12 g of a light yellow product, and the purity of 1,2,3,4,6-O-pentagalloglucose was 98.5% as determined by HPLC.

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Abstract

The invention discloses a method for separating and preparing 1, 2, 3, 4, 6-O-pentagallo-glucose from Fructus alba Fragrant fruit water extract; the water extract is adsorbed by macroporous resin; eluted with alcohol of different volume percentage concentration in turn, collected the eluted part of pure alcohol, evaporated and concentrated to remove the organic solvent, and dried to obtain 1, 2, 3, 4, 6-O-pentagalloglucose, the purity determined by HPLC is >98%. The method has simple process and convenient operation, and the prepared 1,2,3,4,6-O-pentagalloglucose has high purity.

Description

Technical field: [0001] The invention relates to a method for extracting, separating and preparing 1, 2, 3, 4, 6-O-pentagallo-glucose by using Fructus fragrans as a raw material, and belongs to the field of forest products chemical industry. Background technique: [0002] 1,2,3,4,6-O-pentagalloyl glucose (1,2,3,4,6-O-pentagalloyl glucose, PGG) is a natural tannin polyphenolic compound widely present in gall , pomegranate, salt bran and subshrub peony and other medicinal plants. 1,2,3,4,6-O-Pentagalloglucose is a hydrolyzed tannin, which is a gallic acid tannin formed by connecting glucose and 5 gallic acids through ester bonds. Pharmacological studies have shown that the compound has various physiological activities, such as anti-tumor, anti-viral, antioxidant, antagonizing endotoxin / lipopolysaccharide, hypoglycemic and lipid-lowering, etc. At present, 1,2,3,4,6-O-pentagalloglucose is mainly extracted and isolated from the branches and leaves of Chinese gallnut and Phyllan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H13/08C07H1/08
CPCC07H13/08C07H1/08
Inventor 张亮亮汪咏梅徐曼胡新宇
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY