A magnetic bead drying protection solution, drying magnetic beads and preparation method thereof
A protective solution and magnetic bead technology, which is applied in biochemical equipment and methods, DNA preparation, microbial measurement/inspection, etc., can solve problems such as difficult resuspension and dispersion, and affect the chemical activity of functional groups on the surface of magnetic beads. Ease of use and control, improved performance, and extended protection
Active Publication Date: 2021-11-09
INTEGRATED BIOSYSTEMS CO LTD +1
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Problems solved by technology
[0009] The above protection solutions are all lyophilized protection solutions, which need to be freeze-dried for the preservation of immunomagnetic beads, while the hydroxyl magnetic beads will cause the aggregation of magnetic beads under freezing, drying and centrifugation conditions, which are not easy to resuspend and disperse, and Affects the chemical activity of functional groups on the surface of magnetic beads
Method used
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Embodiment 1
[0048] Example bead composition of the present embodiment of the protection liquid drying: 10% cellobiose, 0.01% Tween 20 and sucrose ester 0.1%, the balance being water, wherein the sucrose esters HLB value of 15.
Embodiment 2
[0050] Example bead composition of the present embodiment is the protection liquid drying: 15% cellobiose, 0.02% Tween 20 and sucrose ester 0.2%, the balance being water. Sucrose ester wherein the HLB is 15.
Embodiment 3
[0052] Example bead composition of the present embodiment is a dry passivation solution: 5% cellobiose, 0.005% Tween 20 and sucrose ester 0.05%, the balance being water. Sucrose ester wherein the HLB is 15.
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The invention relates to the technical field of biochemical reagents, in particular to a magnetic bead drying protection solution, a drying magnetic bead and a preparation method thereof. Wherein, the magnetic bead drying protection solution, in terms of mass percentage, comprises 5-15% cellobiose, 0.005-0.02% Tween 20 and 0.05-0.2% sucrose ester, and the balance is water; the The HLB value of the sucrose ester is 15. The magnetic bead drying protection solution provided by the present invention can protect the performance of the dried hydroxyl magnetic beads to the maximum extent, and the dried hydroxyl magnetic beads can be stored and transported at room temperature, and the storage period is long, which is helpful for the microfluidic chip. application.
Description
Technical field [0001] The present invention relates to the technical field of biochemical reagents, particularly, to a passivation solution dry beads, magnetic beads and preparation drying. Background technique [0002] Molecular biological techniques nucleic acid is one of the common research tools to detect pathogenic microorganisms, species identification, origin of species, biodiversity assessments and genetic relationship, phylogenetic and so on. Whether the extracted nucleic acid is the key to high molecular biology experiments, the sensitivity of extraction methods, the specificity is also directly related to the success of subsequent tests. Thus, nucleic acid extraction is one of the most important methods of molecular biology. [0003] With the wide application of molecular biology, nucleic acid extraction technology also proposed new requirements, efficient, convenient, environmentally friendly, high-throughput, automated nucleic acid extraction technology has become t...
Claims
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IPC IPC(8): C12N15/10C12Q1/6806
CPCC12N15/1013C12Q1/6806C12Q2565/629C12Q2563/143C12Q2563/149
Inventor 张瑜蔡亦梅贾欣月李洁昆蒋鹏翀高静任鲁风
Owner INTEGRATED BIOSYSTEMS CO LTD
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