Mulberry leaf fermented feed and preparation method thereof
A technology for fermenting feed and mulberry leaves, applied in the agricultural field, can solve the problems of providing technical solutions for the degradation of mulberry leaf tannins and the like
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[0027] The present invention will be further described in detail below in conjunction with examples, but the embodiments of the present invention are not limited thereto.
[0028] Part 1: Orthogonal experiment to obtain and verify the optimal formula
[0029] 1 Materials and methods
[0030] 1.1 Materials
[0031] Mulberry leaves: from the Huadu base of Guangdong Academy of Agricultural Sciences. The measured dry matter, crude protein, and crude fat contents were 30.50%, 23.67%, and 2.52%, respectively, and the tannin content was 13.21g / kg.
[0032] Pediococcus cellar (CGMCC1.3787) was purchased from the China General Microorganisms Culture Collection and Management Center, and Bacillus licheniformis and Corynespora portuguese were both from the Institute of Animal Science, Guangdong Academy of Agricultural Sciences.
[0033] 1.2 Main reagents and instruments
[0034] MRS broth medium, nutrient broth medium, and potato dextrose medium were purchased from Guangdong Huankai ...
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