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Lactobacillus casei for improving pathological characteristics of psoriasis-like mice and application of lactobacillus casei

A technology of Lactobacillus casei and psoriasis, applied in the field of microorganisms, can solve the problems associated with side effects and psoriasis treatment problems, and achieve the effect of improving pathological characteristics and alleviating psoriasis

Active Publication Date: 2021-06-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Psoriasis currently has no complete cure for medical methods and drugs, and most of them are accompanied by side effects, so the treatment of psoriasis is still a worldwide medical problem

Method used

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  • Lactobacillus casei for improving pathological characteristics of psoriasis-like mice and application of lactobacillus casei
  • Lactobacillus casei for improving pathological characteristics of psoriasis-like mice and application of lactobacillus casei
  • Lactobacillus casei for improving pathological characteristics of psoriasis-like mice and application of lactobacillus casei

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1: the cultivation of Lactobacillus casei and Lactobacillus paracasei

[0055] After inserting Lactobacillus casei CCFM1074 into MRS solid medium and culturing at 37°C for 24 hours, the colonies were observed and observed under a microscope. It was found that the colonies were milky white, irregular, round and smooth, and their Slightly irregular-shaped, round-ended campylobacters, usually present singly, in pairs, and in small clusters.

[0056] Insert Lactobacillus casei CCFM1074 into MRS liquid medium and culture at 37°C for 24 hours, then transfer to fresh MRS liquid medium, cultivate under the same conditions for 12 hours, centrifuge the bacteria at 6000×g for 15 minutes, wash the bacteria with 0.9% normal saline Afterwards, centrifuge again at 6000×g for 10 min, collect the bacteria, resuspend with 30% (m / v) sucrose solution, and freeze and store at 80°C until use.

[0057] Optionally, when Lactobacillus casei is used for gavage to mice, the supernatan...

Embodiment 2

[0059] Embodiment 2: the alleviating effect of lactobacillus casei to psoriasis mouse lesion skin

[0060] Divide 6-8 weeks old SPF grade BALB / c female mice into 4 groups, namely normal group, model group and experimental group, among them, the experimental group includes the CCFM1074 group of Lactobacillus casei CCFM1074 and the para-casein milk group The CCFM1147 group of Bacillus CCFM1147, 6 animals in each group, were raised in the Experimental Animal Center of Jiangnan University, fed with common feed, with a constant temperature of 21-26°C, humidity of 40-70%, noise less than or equal to 60dB, and animal illumination of 15-20LX (all animal experiments All procedures were reviewed and approved by the Animal Welfare and Ethics Management Committee of Jiangnan University).

[0061] The experimental period was 3 weeks in total, and the model was made in the third week. The back of the mouse was depilated the day before the model was made, and the area was about 2.5cm×2.5cm. ...

Embodiment 3

[0064] Embodiment 3: the alleviating effect of Lactobacillus casei to psoriasis mouse lesion ear thickening

[0065] Divide 6-8 weeks old SPF grade BALB / c female mice into 4 groups, namely normal group, model group and experimental group, among them, the experimental group includes the CCFM1074 group of Lactobacillus casei CCFM1074 and the para-casein milk group The CCFM1147 group of Bacillus CCFM1147, 6 animals in each group, were raised in the Experimental Animal Center of Jiangnan University, fed with common feed, with a constant temperature of 21-26°C, humidity of 40-70%, noise less than or equal to 60dB, and animal illumination of 15-20LX (all animal experiments All procedures were reviewed and approved by the Animal Welfare and Ethics Management Committee of Jiangnan University).

[0066] The experimental period was 3 weeks in total, and the model was made in the third week. The back of the mouse was depilated the day before the model was made, and the area was about 2.5...

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Abstract

The invention discloses lactobacillus casei for improving pathological characteristics of psoriasis-like mice and application of the lactobacillus casei, and belongs to the technical field of microorganisms and the technical field of medicines. The invention provides a novel application of lactobacillus casei CCFM1074 serving as an oral preparation or a main component of the oral preparation, and the lactobacillus casei CCFM1074 is used for improving pathological characteristics of a psoriasis-like mouse and relieving psoriasis. The strain can improve the condition of psoriasis-like lesion skin, inhibit thickening of psoriasis-like skin epidermis, inhibit thickening of lesion ears, reduce the level of IL-23 in psoriasis-like skin by 39.1%, reduce the level of IL-17 by 20.2%, increase the level of acetic acid in colon contents by 75.2% in a form of oral administration. The method has a huge application prospect in preparation of products (such as food, medicines or health food) for preventing and / or treating psoriasis.

Description

technical field [0001] The invention relates to a strain of Lactobacillus casei for improving the pathological characteristics of psoriasis-like mice and an application thereof, belonging to the technical field of microbes and the technical field of medicine. Background technique [0002] Psoriasis is an immune-mediated chronic relapsing erythematosquamous skin disease that most commonly affects the skin and joints but is also associated with cardiovascular, metabolic, and neuropsychiatric effects. Psoriasis affects about 2% of the world's population, but there are marked differences in prevalence among different ethnic groups. The mean time from onset of psoriasis symptoms to diagnosis was 2 years, and the mean age at diagnosis was 34 years. The prevalence of psoriasis appears to be higher in countries with higher latitudes. As a skin disease, psoriasis can manifest in a variety of phenotypes, including plaque psoriasis (also known as psoriasis vulgaris), punctate psorias...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61P17/06A23C9/123A23L11/50A23L19/00A23L2/52A23L2/385A23L33/135A23K10/18A23K50/50
CPCA61K35/747A61P17/06A23C9/1238A23C9/1234A23L19/00A23L2/52A23L2/385A23L33/135A23K10/18A23K50/50A23V2002/00A23V2400/125A23V2200/318
Inventor 陆文伟陈卫翟齐啸杨波邓雅丹崔树茂赵建新张灏
Owner JIANGNAN UNIV
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