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Bifidobacterium longum subsp. Infantis CCFM1192 strain, leavening agent and preparation method and application thereof

A technology of CCFM1192 and CFM1192, applied in the field of microorganisms, can solve the problems of recurrent asthma, emphysema, and poor patient compliance, and achieve the effect of improving pathological characteristics, relieving asthma, and having great application prospects.

Pending Publication Date: 2022-04-05
BRIGHT DAIRY & FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the drugs for the treatment of asthma include bronchodilator drugs, anti-inflammatory drugs and probiotics. Among them, most of the bronchodilator drugs and anti-inflammatory drugs need to be given by intravenous injection or inhalation, which leads to poor compliance of patients, and asthma Repeated attacks, and even serious complications such as emphysema

Method used

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  • Bifidobacterium longum subsp. Infantis CCFM1192 strain, leavening agent and preparation method and application thereof
  • Bifidobacterium longum subsp. Infantis CCFM1192 strain, leavening agent and preparation method and application thereof
  • Bifidobacterium longum subsp. Infantis CCFM1192 strain, leavening agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1: Screening, morphological observation and identification of Bifidobacterium longum subsp. infantis

[0055] Take 1g of fresh feces from healthy infants, spread it on mMRS solid medium containing 1% mupirocin after gradient dilution, and culture in an anaerobic environment at 37°C for 72 hours; Second, after culturing at 37°C for 48 hours, the colonies were observed and observed under a microscope, and it was found that the colonies were milky white, rounded, convex, smooth, and the shape of the bacteria was slightly irregular, with rounded ends, usually Single and small clusters exist.

[0056] The strains obtained from the above screening were cultivated in fresh mMRS liquid culture at 37°C for 24 hours, and then 1 mL of the bacterial liquid was centrifuged at 3500 × g to obtain the bacterial sludge, and then 1 mL of sterile water was added to wash the bacterial sludge, and the supernatant was removed by centrifugation under the same conditions. Then add 1...

Embodiment 2

[0064] Example 2: Culture of Bifidobacterium longum subsp. infantis

[0065] Introduce Bifidobacterium longum subsp. infantis CFM1192 into mMRS liquid medium and culture at 37°C for 24 hours, then transfer to fresh mMRS liquid medium, cultivate under the same conditions for 16-24 hours, centrifuge the bacteria at 6000×g for 15 minutes, and use 0.9 After washing the cells with % normal saline, they were centrifuged again at 6000×g for 10 min to collect the cells, resuspended with 30% (m / v) sucrose solution, and frozen in a -80°C refrigerator until use.

[0066] When the Bifidobacterium longum subsp. infantis strain is used for intragastric administration to mice, the supernatant is centrifuged after being taken out from a -80° C. refrigerator, and resuspended with normal saline to obtain the probiotic suspension used for intragastric administration.

Embodiment 3

[0067] Example 3: Bifidobacterium longum subsp. infantis CCFM1192 significantly reduces pathology scores in asthmatic mice

[0068] The 6-week-old male SPF grade BABL / c mice were divided into 5 groups, namely blank group, model group, positive group (Lactobacillus reuteri CCFM1040), Z1M3 group and CCFM1192, with 8 mice in each group. Animal center, fed with common feed, constant temperature 21-26°C, humidity 40-70%, noise less than or equal to 60dB, animal illumination 15-20LX (all animal experiment procedures are reviewed and approved by the Animal Welfare and Ethics Management Committee of Jiangnan University) .

[0069] The experimental period was 5 weeks in total. The mice were administrated after 7 days of adaptation. From the 8th day to the 35th day, the blank group and the model group were administrated with 0.2 mL of normal saline solution, and the positive control group, CCFM1192 group and Z1M3 group were administrated with 0.2 mL of normal saline solution. The bac...

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Abstract

The invention discloses a bifidobacterium longum subspecies infantis CCFM1192 strain, a leavening agent, a preparation method of the bifidobacterium longum subspecies infantis CCFM1192 strain and application of the bifidobacterium longum subspecies infantis CCFM1192 strain and the leavening agent. The strain is named as bifidobacterium longum subspecies infantis CCFM1192 in taxonomy, and the preservation number of the strain is GDMCC No.61846. The invention further discloses a preparation method of the bifidobacterium longum subspecies infantis CCFM1192. The preparation method of the leavening agent comprises the following steps: (1) inoculating a culture medium with bifidobacterium longum subsp. Infantis CFM1192, and culturing to obtain a culture solution; (2) centrifuging the culture solution, and collecting to obtain thalli; (3) cleaning the thalli, and resuspending the thalli by using a freeze-drying protective agent to obtain a resuspension solution; and (4) freeze-drying the resuspension by adopting a vacuum freezing method to obtain the leavening agent. The bifidobacterium longum subsp. Infantis CCFM1192 and the leavening agent provided by the invention can improve the pathologic characteristics of asthma so as to relieve asthma, and are applied to the fields of foods, medicines or health foods and the like.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Bifidobacterium longum subsp. infantis CCFM1192 bacterial strain, a fermentation agent and a preparation method and application thereof. Background technique [0002] Asthma is an immune-mediated chronic airway inflammation, which is caused by a variety of cells, especially mast cells, eosinophils and T lymphocytes. With the continuous advancement of the industrialization process, the global incidence of allergic diseases, including asthma, is gradually increasing. The global average prevalence of asthma is about 1%, and among them, children can reach as high as 3%. Asthma can cause recurrent symptoms such as wheezing, shortness of breath, chest tightness and / or coughing, which can lead to complications such as respiratory arrest and respiratory failure, which endanger the lives of patients. Asthma has become a major allergic disease that seriously threatens public heal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/123A23C13/16A23C19/032A23C19/06A23L11/50A23L29/00A23L33/00A61K35/745A61P11/06C12R1/01
Inventor 陆文伟刘小鸣张灏方志锋
Owner BRIGHT DAIRY & FOOD
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