Application of bacillus coagulans JA845 in preparation of medicine for preventing and/or treating Alzheimer's disease
A technology for Bacillus coagulans and Alzheimer's disease, applied in drug combinations, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as inability to cure AD, reduce neuronal degeneration, and reduce learning and memory-related problems. Injury, improve the effect of AD pathological features
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[0052] The preparation method of D-galactose solution: take D-galactose powder and prepare D-galactose solution with a concentration of 12mg / ml with physiological saline.
[0053] Aluminum chloride: purchased from Sinopharm Chemical Reagent Co., Ltd.
[0054] The preparation method of the aluminum chloride solution: take the aluminum chloride powder and prepare an aluminum chloride solution with a concentration of 2 mg / ml with physiological saline.
Embodiment 1
[0055] The preparation of embodiment 1 bacillus coagulans (Bacillus coagulans) JA845 bacterial suspension
[0056] 1. Strain activation: Pick the freeze-dried bacterial powder of Bacillus coagulans (Bacillus coagulans) JA845, inoculate it in LB liquid medium, and cultivate the activated strain in a shaking incubator at 50°C and 180 rpm for 8-9 hours.
[0057] 2. Subculture of the strain: take the activated culture medium of the strain, inoculate it into a new LB liquid medium with a 3% inoculation amount, culture it in a shaking incubator at 50°C and 180rpm for 8-9 hours, repeat this step to passage the strain Repeat this step 3 times to expand the culture to obtain more bacterial liquid.
[0058] 3. Calculation of the number of viable bacteria: Dilute the bacterial solution by 10 and 10 respectively by coating plate counting method 2 、10 3 、10 4 、10 5 、10 6 、10 7 、10 8 and 10 9 times, the diluted bacterial solution was evenly spread on the surface of LB solid medium, ...
Embodiment 2
[0060] Example 2 Effect of Bacillus coagulans (Bacillus coagulans) JA845 on preventing and treating Alzheimer's disease
[0061] 1. Materials and methods:
[0062] 1. Establishment of Alzheimer's disease mouse model and experimental grouping
[0063] 30 SPF-grade ICR mice, half male and half female, weighing 20±5 (g), kept at constant temperature (20±2°C) and constant humidity (40-60%), kept circadian rhythm for 12 hours, free to eat and drink, and fed normally for one week , were randomly divided into 3 groups according to body weight and sex: blank control group (n=10), AD model group (n=10), JA845 prevention group (n=10), AD model group was fed with sterile normal saline every day, 90min Afterwards, intraperitoneal injection of D-galactose solution (120mg / kg), intragastric administration of AlCl 3 solution (20mg / kg); the JA845 prevention group was fed intragastrically with Bacillus coagulans JA845 bacterial suspension every day, and after 90min, intraperitoneally injected...
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