Moringa oleifera leaf methanol extract capable of inhibiting trypanosoma brucei of pigs

A technology of Trypanosoma brucei and its extract, which is applied in the direction of drug combination, plant raw materials, medical preparations containing active ingredients, etc., can solve side effects and other problems, and achieve the goal of reducing neuron degeneration and congestion, good efficacy and safety Effect

Inactive Publication Date: 2019-03-01
DINGZHENG ANIMAL PHARMA TIANJIN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional chemotherapy for trypanosomiasis often comes with side effects

Method used

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  • Moringa oleifera leaf methanol extract capable of inhibiting trypanosoma brucei of pigs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A natural extract of Moringa oleifera leaves for the treatment of Trypanosoma brucei in pigs, directly crushing Moringa oleifera leaves for use.

[0016] A Moringa oleifera extract for treating pig trypanosomiasis as claimed in claim 1, characterized in that: the steps of the preparation method are as follows:

[0017] Wash Moringa leaves thoroughly, dry in the shade and crush into powder.

Embodiment 2

[0019] A natural extract of Moringa oleifera leaves for the treatment of Trypanosoma brucei in pigs, concentrated to a concentration of 10% using a rotary evaporator.

[0020] A Moringa oleifera extract for treating pig trypanosomiasis as claimed in claim 1, characterized in that: the steps of the preparation method are as follows:

[0021] Wash Moringa leaves thoroughly, dry in the shade and crush into powder.

[0022] The obtained Moringa oleifera leaf powder was concentrated to a final concentration of 10% by using a rotary evaporator, and the concentrated product was diluted in soybean oil as a carrier to prepare a natural medicine capable of inhibiting trypanosomiasis brucei.

Embodiment 3

[0024] A natural extract of Moringa oleifera leaves for the treatment of Trypanosoma brucei in pigs, concentrated to a concentration of 7.5% using a rotary evaporator.

[0025] The steps of its preparation method are the same as in Example 2

[0026] Clinical application:

[0027] 1. Materials and Methods

[0028] The method of extraction of Moringa oleifera extract: Moringa oleifera leaves were washed thoroughly, dried in a cool place and pulverized into powder, the extract was concentrated to a final concentration of 7.5% stock solution using a rotary evaporator, and the product was diluted in large in soybean oil.

[0029] Trypanosome inoculation; the dose of the inoculum was judged to be 3 x 10 per mL of blood 6 The Trypanosoma brucei. Maintenance of the parasite by serial passage in pigs. To infect pigs, 1 ml of blood was collected from heavily infected pigs and fused with 2 ml of saline. The mixture was observed under a microscope at X40 magnification. Blood contai...

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Abstract

The invention relates to a moringa oleifera leaf extract for treating trypanosoma brucei of pigs. The extract is used as a natural drug with effective ingredients of flavonoids, tannins, saponins, steroids, phenols carbohydrates and glycosides according to chemical analysis. The pigs infected with the trypanosoma bruce have weight loss and die within 15 days of infection. At present, chemical methods are mainly adopted for treatment and prevention in the market, but there is a common problem that chemical drugs have drug resistance. With a view to screening of natural anti-parasitic drugs, moringa oleifera leaves are selected and crushed to obtain the moringa oleifera leaf extract to treat the trypanosoma brucei. Experiments show that degeneration of brain neuron cells caused by the trypanosoma brucei can be effectively controlled 14days after acute dosing before infection with the trypanosoma brucei. The extract contributes to weight gain of the pigs. Therefore, the natural drug is agood lead drug for anti-parasitic diseases. On this basis, the trypanosoma brucei can be well treated by further separation of the active ingredients.

Description

technical field [0001] The invention belongs to the technical field of livestock medicine, in particular to a methanol extract of Moringa oleifera which can inhibit Trypanosoma brucei. Background technique [0002] Trypanosomiasis is a major disease of humans and animals. Especially parasitic diseases of pigs, manifested in the form of: severe anemia, weight loss, decreased productivity, infertility and abortion, death in acute and chronic periods, etc. Curative and preventive medicines (chemotherapy and chemoprevention) are the main methods of controlling trypanosomiasis. Despite problems with drug resistance and toxicity, these methods of control remain the most reliable, especially since despite extensive research efforts, an effective vaccine against the disease has not yet been successfully developed. Conventional chemotherapy for trypanosomiasis often has side effects. Current research focuses on new compounds for the treatment of trypanosome infections. One possib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P33/02A61K127/00
CPCA61K36/185A61K2236/33A61K2236/51A61P33/02
Inventor 刘鼎阔董惠峰刘芳李源胡鹏程
Owner DINGZHENG ANIMAL PHARMA TIANJIN
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