Moringa oleifera leaf methanol extract capable of inhibiting trypanosoma brucei of pigs
A technology of Trypanosoma brucei and its extract, which is applied in the direction of drug combination, plant raw materials, medical preparations containing active ingredients, etc., can solve side effects and other problems, and achieve the goal of reducing neuron degeneration and congestion, good efficacy and safety Effect
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Embodiment 1
[0015] A natural extract of Moringa oleifera leaves for the treatment of Trypanosoma brucei in pigs, directly crushing Moringa oleifera leaves for use.
[0016] A Moringa oleifera extract for treating pig trypanosomiasis as claimed in claim 1, characterized in that: the steps of the preparation method are as follows:
[0017] Wash Moringa leaves thoroughly, dry in the shade and crush into powder.
Embodiment 2
[0019] A natural extract of Moringa oleifera leaves for the treatment of Trypanosoma brucei in pigs, concentrated to a concentration of 10% using a rotary evaporator.
[0020] A Moringa oleifera extract for treating pig trypanosomiasis as claimed in claim 1, characterized in that: the steps of the preparation method are as follows:
[0021] Wash Moringa leaves thoroughly, dry in the shade and crush into powder.
[0022] The obtained Moringa oleifera leaf powder was concentrated to a final concentration of 10% by using a rotary evaporator, and the concentrated product was diluted in soybean oil as a carrier to prepare a natural medicine capable of inhibiting trypanosomiasis brucei.
Embodiment 3
[0024] A natural extract of Moringa oleifera leaves for the treatment of Trypanosoma brucei in pigs, concentrated to a concentration of 7.5% using a rotary evaporator.
[0025] The steps of its preparation method are the same as in Example 2
[0026] Clinical application:
[0027] 1. Materials and Methods
[0028] The method of extraction of Moringa oleifera extract: Moringa oleifera leaves were washed thoroughly, dried in a cool place and pulverized into powder, the extract was concentrated to a final concentration of 7.5% stock solution using a rotary evaporator, and the product was diluted in large in soybean oil.
[0029] Trypanosome inoculation; the dose of the inoculum was judged to be 3 x 10 per mL of blood 6 The Trypanosoma brucei. Maintenance of the parasite by serial passage in pigs. To infect pigs, 1 ml of blood was collected from heavily infected pigs and fused with 2 ml of saline. The mixture was observed under a microscope at X40 magnification. Blood contai...
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