Application of KLC3 gene as marker in diagnosis and treatment of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer

A technology for endometrial cancer and colorectal cancer, applied in the fields of molecular biology and tumor marker medicine, can solve problems such as unclear effects, and achieve the effect of inhibiting tumor cell invasion ability, increasing apoptosis level, and inhibiting cell proliferation ability. Effect

Pending Publication Date: 2021-07-16
THE THIRD AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIVERSITY
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Problems solved by technology

However, its role in tumors is still unclear, and there is no relevant literature reporting the role of this gene. Effective early onset biomarkers and related molecular mechanisms have b

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  • Application of KLC3 gene as marker in diagnosis and treatment of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer
  • Application of KLC3 gene as marker in diagnosis and treatment of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer
  • Application of KLC3 gene as marker in diagnosis and treatment of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer

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[0043] EXAMPLES Expression of KLC3 gene in cell lines of malignant tumors (including lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer) and related cell function test methods.

[0044] Sources of cell lines involved in the examples: gastric cancer cell lines (MGC-803, SGC7901), intestinal cancer cell lines (HCT116, SW480, SW620), lung cancer cell lines (A549, H1299, SPC-1A), endometrial cancer cell lines Lines (H1C-1A, H1C-1B, Ishikuwa), ovarian cancer cell lines (A2780, CAOV3, OVCAR3, SKOV3, HO8910) were purchased from Guangzhou Geneo Biotechnology Co., Ltd.

[0045] 1. Identification of KLC3 mRNA levels in lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer cell lines.

[0046] 1.1 Use Trizol reagent to extract the total RNA of tumor cells, add 1 / 5 volume of Trizol in chloroform, shake vigorously for 15 seconds, after the solution is fully emulsified, let stand at room temperature for 5 minutes; at 4°C, ce...

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Abstract

The invention relates to the technical field of molecular biology and tumor marker medicine, in particular to application of a KLC3 gene as a marker in diagnosis and treatment of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer. The KLC3 gene can be used as a diagnostic biomarker for lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer or can be used for preparing a cancer diagnosis product. The KLC3 gene is specifically overexpressed in lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer, but there is no such specific high expression in normal tissues. By detecting the expression of the KLC3 gene in a subject, the occurrence of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer can be quickly, accurately and clearly determined. The KLC3 gene serves as a diagnostic marker of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer, provides a new target for clinical treatment of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer, and can be applied to preparation of antitumor drugs.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and tumor marker medicine, in particular to the application of KLC3 gene as a marker in the diagnosis and treatment of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer. Background technique [0002] Lung cancer (LC) is the most common malignant tumor worldwide. From the perspective of pathology and treatment, lung cancer can be roughly divided into two categories: non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC), of which non-small cell lung cancer accounts for about 80% to 85%. , and the rest were small cell lung cancers. Due to the unique biological behavior of small cell lung cancer, except for a few early-stage cases, comprehensive treatment combined with chemotherapy and radiotherapy is mainly used. Lung cancer refers to non-small cell lung cancer unless otherwise specified. Lung cancer is the malignant tumor with the fast...

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11A61K45/00A61P35/00
CPCC12Q1/6886A61K45/00A61P35/00C12Q2600/118C12Q2600/158C12Q2600/136
Inventor 赵杨张松沈帆苏桂锋
Owner THE THIRD AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIVERSITY
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