Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of KLC3 gene as marker in diagnosis and treatment of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer

A technology for endometrial cancer and colorectal cancer, applied in the fields of molecular biology and tumor marker medicine, can solve problems such as unclear effects, and achieve the effect of inhibiting tumor cell invasion ability, increasing apoptosis level, and inhibiting cell proliferation ability. Effect

Pending Publication Date: 2021-07-16
THE THIRD AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIVERSITY
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its role in tumors is still unclear, and there is no relevant literature reporting the role of this gene. Effective early onset biomarkers and related molecular mechanisms have been found to guide lung cancer, gastric cancer, colorectal cancer, and endometrial cancer. Cancer and ovarian cancer clinical diagnosis, treatment and prognosis are of great significance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of KLC3 gene as marker in diagnosis and treatment of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer
  • Application of KLC3 gene as marker in diagnosis and treatment of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer
  • Application of KLC3 gene as marker in diagnosis and treatment of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0043] EXAMPLES Expression of KLC3 gene in cell lines of malignant tumors (including lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer) and related cell function test methods.

[0044] Sources of cell lines involved in the examples: gastric cancer cell lines (MGC-803, SGC7901), intestinal cancer cell lines (HCT116, SW480, SW620), lung cancer cell lines (A549, H1299, SPC-1A), endometrial cancer cell lines Lines (H1C-1A, H1C-1B, Ishikuwa), ovarian cancer cell lines (A2780, CAOV3, OVCAR3, SKOV3, HO8910) were purchased from Guangzhou Geneo Biotechnology Co., Ltd.

[0045] 1. Identification of KLC3 mRNA levels in lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer cell lines.

[0046] 1.1 Use Trizol reagent to extract the total RNA of tumor cells, add 1 / 5 volume of Trizol in chloroform, shake vigorously for 15 seconds, after the solution is fully emulsified, let stand at room temperature for 5 minutes; at 4°C, ce...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of molecular biology and tumor marker medicine, in particular to application of a KLC3 gene as a marker in diagnosis and treatment of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer. The KLC3 gene can be used as a diagnostic biomarker for lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer or can be used for preparing a cancer diagnosis product. The KLC3 gene is specifically overexpressed in lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer, but there is no such specific high expression in normal tissues. By detecting the expression of the KLC3 gene in a subject, the occurrence of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer can be quickly, accurately and clearly determined. The KLC3 gene serves as a diagnostic marker of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer, provides a new target for clinical treatment of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer, and can be applied to preparation of antitumor drugs.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and tumor marker medicine, in particular to the application of KLC3 gene as a marker in the diagnosis and treatment of lung cancer, gastric cancer, colorectal cancer, endometrial cancer and ovarian cancer. Background technique [0002] Lung cancer (LC) is the most common malignant tumor worldwide. From the perspective of pathology and treatment, lung cancer can be roughly divided into two categories: non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC), of which non-small cell lung cancer accounts for about 80% to 85%. , and the rest were small cell lung cancers. Due to the unique biological behavior of small cell lung cancer, except for a few early-stage cases, comprehensive treatment combined with chemotherapy and radiotherapy is mainly used. Lung cancer refers to non-small cell lung cancer unless otherwise specified. Lung cancer is the malignant tumor with the fast...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6886C12N15/11A61K45/00A61P35/00
CPCC12Q1/6886A61K45/00A61P35/00C12Q2600/118C12Q2600/158C12Q2600/136
Inventor 赵杨张松沈帆苏桂锋
Owner THE THIRD AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com