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Hypersensitive cardiac troponin I detection method and kit

A cardiac troponin and detection kit technology, applied in the field of protein detection, can solve the problems of missed detection results, unsatisfactory detection rate, and failure to meet the preliminary screening and prediction of patients' condition, so as to reduce the risk of missed detection , the effect of improving the detection rate

Active Publication Date: 2021-07-16
GUANGDONG WESAIL BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The kits developed on the market using the detection principle of antigen-antibody specific binding mostly use antibody pairing against cTnI or cTnT single antigens, which cannot capture the most comprehensive forms of cardiac troponin in the body, which may easily cause missed detection and The test results cannot meet the effect of preliminary screening and prediction of the patient's condition, nor can it meet the requirements for the detection rate of normal people

Method used

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  • Hypersensitive cardiac troponin I detection method and kit
  • Hypersensitive cardiac troponin I detection method and kit
  • Hypersensitive cardiac troponin I detection method and kit

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Embodiment Construction

[0061] The present invention will be described in further detail below mainly in conjunction with specific embodiments. The following examples are provided to illustrate embodiments of the invention and are not intended to limit the invention. The invention may optionally include embodiments not shown by way of example.

[0062] 1. Preparation of cardiac troponin I monoclonal antibody

[0063] 1. Immune animals: take BALB / c mice of the same strain as myeloma cells at the age of 8 to 12 weeks, and use cTnI antigen (SEQ ID NO: 1) fully mixed with an equal amount of Freund's complete adjuvant to give 100 μg of antigen The protein / mouse was injected into the intraperitoneal cavity of the mouse, and the cTnI antigen, which was thoroughly mixed with the same amount of Freund's incomplete adjuvant, was injected into the intraperitoneal cavity of the mouse several times to boost the immunization with 100 μg antigenic protein / mouse. After the detection of mouse serum (indirect ELISA ...

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Abstract

The invention relates to a hypersensitive cardiac troponin I detection method and a kit. The kit comprises a first group of antibodies and a second group of antibodies which are used for detecting cardiac troponin I in a sample of a subject, wherein the antibodies are selected from the following antibodies: an antibody 1: 41st-49th amino acid fragments specifically binding to cardiac troponin I, an antibody 2: 83rd to 92nd amino acid fragments specifically binding to cardiac troponin I, an antibody 3: 169th to 178th amino acid fragments specifically binding to cardiac troponin I, and an antibody 4: an antibody specifically binding to a cardiac troponin compound cTnC-T-I. The method and the kit disclosed by the invention are high in sensitivity and simple to operate, and can be used for dynamically monitoring cardiac troponin I.

Description

technical field [0001] The invention relates to the field of protein detection. Specifically, the present invention relates to a detection method and a kit for hypersensitive cardiac troponin I. Background technique [0002] Troponin is a marker of myocardial injury and necrosis, and it has important clinical significance for the diagnosis and risk stratification of acute myocardial infarction (100% diagnostic sensitivity, 91% specificity, and long duration), cardiac troponin (cTn ) structure is composed of three subunits, including cardiac troponin I (cTnI), cardiac troponin C (cTnC) and cardiac troponin T (cTnT), abnormal cTn can be measured 6-8 hours after myocardial injury, increased Lasts for 1 to 2 weeks. cTn is released in the intact form (cTnC-cTnI-cTnT triple subunit complex form, or called cardiac troponin complex cTnC-T-I) during myocardial injury, and then degraded; cTnI and cTnC in peripheral blood are complexed as cTnI-cTnC The physical form is the majority ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577C07K16/18
CPCG01N33/6893G01N33/6887G01N33/577C07K16/18G01N2333/4712G01N2800/324C07K2317/34
Inventor 于鸫刘丽萍李然迟晨覃素妮卢盛萍王凯黄春连李文静李斌何智丹
Owner GUANGDONG WESAIL BIOTECH CO LTD
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