Mass spectrometry method of TIMP1 protein standard substance

A technology of mass spectrometry and reference material, which is applied in the field of mass spectrometry analysis of matrix metalloproteinase inhibitor 1 standard material, can solve the problems of poor selectivity, low sensitivity and accuracy, and achieve the effect of reducing interference

Pending Publication Date: 2021-08-06
HANGZHOU GUANGKEANDE BIOTECHNOLOGY CO LTD
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  • Claims
  • Application Information

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Problems solved by technology

Commonly used protein concentration determination methods such as Coomassie brilliant blue method, Folin-phenol reagent method, and BCA method have the disadvantages of poor selectivity, low sensitivity and accuracy

Method used

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  • Mass spectrometry method of TIMP1 protein standard substance
  • Mass spectrometry method of TIMP1 protein standard substance
  • Mass spectrometry method of TIMP1 protein standard substance

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Embodiment Construction

[0049] In order to enable those skilled in the art to better understand the solution of the present application, the technical solution in the embodiment of the application will be clearly and completely described below in conjunction with a case. Obviously, the described embodiment is only a part of the embodiment of the application. rather than all examples. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without creative efforts shall fall within the scope of protection of this application.

[0050] A method for mass spectrometry analysis of a TIMP1 protein standard substance, comprising the following steps:

[0051] (1) Select two characteristic peptides GFQALGDAADIR (TIMP1a) and SEEFLIAGK (TIMP1b) for TIMP1 protein quantification;

[0052] (2) Using stable isotopes 13 C and 15 N labels the arginine of the above characteristic peptide GFQALGDAADIR to obtain the corresponding internal standard peptide G...

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Abstract

The invention discloses a mass spectrometry method of a TIMP1 protein standard substance, which has the beneficial effects that the TIMP1 standard substance is subjected to absolute quantitative analysis by using an isotope dilution mass spectrometry for the first time, the blank in the prior art is filled, and the mass spectrometry method has important significance on traceability analysis research and quality control system establishment of the TIMP1 standard substance; according to the method, a mass spectrum parallel reaction monitoring mode is combined, data are collected under high resolution precision, and compared with a conventional multi-reaction monitoring mode (MRM), interference of matrix co-effluents on peptide fragment analysis is remarkably reduced; and the analysis method provided by the invention has guidance and reference significance for value determination and traceability analysis research of other protein standard substances.

Description

technical field [0001] The invention relates to a protein analysis and detection technology, in particular to a mass spectrometry analysis method for a matrix metalloproteinase inhibitor 1 (TIMP1 protein) standard substance. Background technique [0002] Matrix metalloproteinase inhibitor 1 (Tissue inhibitor of metalloproteinases 1, TIMP1) can be widely involved in tissue remodeling, tumor angiogenesis, tumor cell invasion and metastasis by inhibiting the activity of metalloproteinases, and is an important early diagnosis of cancer. and prognostic biomarkers. [0003] The reliability of the quantitative determination of TIMP1 in clinical testing depends on the accuracy of the determination of the protein standard substance. Commonly used protein concentration determination methods such as Coomassie brilliant blue method, Folin-phenol reagent method, and BCA method have the disadvantages of poor selectivity, low sensitivity and accuracy. Mass spectrometry is a new technolog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 高俊顺江南宋林珍王洪高俊莉
Owner HANGZHOU GUANGKEANDE BIOTECHNOLOGY CO LTD
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