Red grape ABA8ox3 gene overexpression vector as well as construction method and application thereof

An overexpression vector and gene overexpression technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problems of grape fruit yield and quality damage, reduced commerciality and other problems, and achieve the effect of reducing the loss of fruit farmers and high commodity value.
CN113234738AInactive Publication Date: 2021-08-10ZHEJIANG WANLI UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG WANLI UNIV
Publication Date
2021-08-10
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses a red grape ABA8ox3 gene overexpression vector as well as a construction method and an application thereof, the overexpression vector is a pCAMBIA1302-ABA8ox3 overexpression vector obtained by inserting a target fragment of a red grape ABA8ox3 gene between a CaMV 35 promoter and an mgfp5 fluorescent sequence of a pCAMBIA1302 original vector, replacing four catg basic groups of the pCAMBIA1302 original vector sequence and deleting a termination codon of the target fragment, and the nucleotide sequence of the overexpression vector is as shown in SEQ ID NO. 1. The pCAMBIA1302-ABA8ox3 constructed by the invention has the capability of overexpressing the ABA8ox3 gene, and the content of endogenous ABA in red grape fruits is directly regulated and controlled by overexpressing the ABA8ox3 gene, so that the mature period of the red grapes is advanced, the picking period of the red grapes can bypass the typhoon season, the loss of fruit farmers is reduced, and the pCAMBIA1302-ABA8ox3 win the same-period competition with other fruits or grapes and obtains higher commodity value.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to an ABA8ox3 gene overexpression vector of Yinhong grape and its construction method and application. Background technique

[0002] Grape (scientific name: Vitis vinifera) is one of the five economical fruit trees in my country. Its fruit has rich nutritional value, fresh and sweet unique taste, and various eating methods, and is widely loved by the Chinese people. However, in southern China, due to the influence of the monsoon climate from July to August, the yield and quality of grape fruit were damaged, and the commerciality decreased. The main grape variety "Yinhong" in Ningbo City, Zhejiang Province was affected by this.

[0003] The hydroxylation catalyzed by ABA8'-hydroxylase can catabolize ABA (abscisic acid, abscisic acid), and this reaction is encoded by the CYP707A gene family. ABA plays a major regulatory role in the fruit ripening process, so the change of endogenous ABA...

Claims

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