Gene editing tobacco single plant hydroponic device and hydroponic method

A gene editing and hydroponic device technology, applied in the field of tobacco cultivation, can solve the problems of cross-infection of pathogens, cross-infection, hydroponic green algae breeding, etc.

A gene editing and hydroponic device technology, applied in the field of tobacco cultivation, can solve the problems of cross-infection of pathogens, cross-infection, hydroponic green algae breeding, etc.

CN113273484APending Publication Date: 2021-08-20CHINA TOBACCO YUNNAN IND
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  • Gene editing tobacco single plant hydroponic device and hydroponic method
  • Gene editing tobacco single plant hydroponic device and hydroponic method
  • Gene editing tobacco single plant hydroponic device and hydroponic method

Examples

Experimental program
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Effect test

Embodiment 1

[0041] A dark-proof hydroponic cup with a diameter of 8cm and a height of 10cm, the cup cover is recessed by 2cm; a transparent liquid level observation tube with a diameter of 0.5cm is connected to the bottom of the hydroponic cup, and a filter is placed at the connection; the diameter of the planting cup is 5cm, height is 5cm, the diameter of the fixed edge of the planting cup is 0.5cm larger than that of the hydroponic cup, and it can bear more than 2kg. Put the prepared nutrient solution into the hydroponic cup, and reach the height of the hydroponic cup at 8cm.

[0042] The gene-edited tobacco and the female parent control tobacco were raised in normal floating. After the tobacco seedlings reached the standard of normal transplanting and strong seedlings, 20 tobacco seedlings with uniform growth and uniform size were selected for transplanting. When transplanting, pull out the tobacco seedlings from the seedling tray, wrap the tobacco roots with black polyester fiber (wra...

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Abstract

The invention discloses a gene editing tobacco single plant hydroponic device and a hydroponic method. The gene editing tobacco single plant hydroponic device comprises a hydroponic cup (4), a field planting cup (3), a water absorption line (7) and a water level observation pipe (8), wherein a hydroponic cup cover (2) is arranged at the top of the hydroponic cup (4), the field planting cup (3) is fixed on the upper part of the hydroponic cup (4) through the hydroponic cup cover (2), and tobacco seedlings (1) and a substrate for culturing the tobacco seedlings (1) are loaded in the field planting cup (3); the water level observation pipe (8) is positioned in the hydroponic cup (4) and is used for observing the water level height of a nutrient solution (5) in the hydroponic cup (4); and the hydroponic cup (4) and the hydroponic cup cover (2) are black and light-proof. A single-plant hydroponic device is constructed, a nutrient solution of each plant is independently added, exchange and sharing of the nutrient solution among plants are avoided, and cross infection of tobacco diseases due to nutrient solution exchange is prevented. In addition, the problem of breeding of hydroponic green algae is solved by selecting a light-proof material or coating a shading material.

Description

technical field [0001] The invention belongs to the field of tobacco cultivation, and in particular relates to a gene-edited tobacco single-plant hydroponic device and a hydroponic method, and its application in gene-edited material screening. Background technique [0002] Tobacco is an important economic crop in my country, and it is also a commonly used model plant for molecular genetics research. Tobacco traits are not only affected by its own genetic material, but also by planting environmental factors. Planting conditions such as different temperature, climate and soil have a great influence on the traits, yield and quality of tobacco. [0003] Hydroponics is a relatively advanced cultivation method in soilless cultivation, which directly contacts the root system of the plant with the nutrient solution. The production of plants that are nourished by a nutrient solution. Hydroponics can precisely control the growth and development of plants and is easy to manage. The...

Claims

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Application Information

Patent Timeline
20 Aug 2021
Publication
CN113273484A
IPC
A01G31/06; A01G31/02; A01G22/45
CPC
A01G31/06; A01G31/02; A01G22/45; Y02P60/21
Inventors
张建铎; 王晋