Nucleic acid multiple detection micro-fluidic chip capable of realizing automatic sample feeding

A microfluidic chip, multiple detection technology, applied in the determination/inspection of microorganisms, enzymology/microbiology devices, bioreactors/fermenters for specific purposes, etc. , environmental interference and other issues, to achieve the effect of preventing mutual interference, wide application range, and avoiding outflow
CN113278510APending Publication Date: 2021-08-20NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
Publication Date
2021-08-20

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Abstract

The invention discloses a nucleic acid multiple detection micro-fluidic chip capable of realizing automatic sample feeding. The chip is a closed chip which is formed by bonding a substrate and a cover plate and is only provided with a sample feeding hole; the substrate is provided with a sample feeding hole, a main sample feeding channel, a reaction unit, a vacuum cavity and a vacuum channel; the reaction unit communicates with the main sample feeding channel through a bridging channel; one end of the main sample feeding channel communicates with the sample feeding hole; the vacuum cavity communicates with the vacuum channel, and the vacuum cavity is separated from the reaction unit through an air-permeable diaphragm; and a sample adding hole matched with the sample feeding hole is formed in the cover plate. According to the design of the nucleic acid multiple detection micro-fluidic chip capable of realizing automatic sample feeding, vacuum pumping is performed during preparation, and the chip is stored in a sealed manner in a vacuum bag; when the chip is used, the vacuum packaging bag is torn, sample feeding can be directly performed, and power devices such as a sample feeding pump are not needed; and the requirements of nucleic acid field detection on instruments and equipment can be greatly reduced.
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Description

technical field

[0001] The invention belongs to the field of nucleic acid detection, relates to the field of microfluidic technology, in particular to a microfluidic chip capable of automatic sampling for multiple nucleic acid detection. Background technique

[0002] Microfluidic chips are also known as "Lab on a Chip" (Lab on a Chip), and their purpose is to integrate sample pretreatment, sample addition, mixing, reaction, separation, detection, etc. involved in biological, chemical, and medical analysis processes. The operating unit is partly or fully integrated on a micron-scale chip, and by controlling the liquid flow in the microchannel of the chip, the rapid, accurate and high-throughput automatic analysis of the analyte can be realized.

[0003] At present, microfluidic chips have been applied in the field of nucleic acid detection. Through the integration of chips, the complex process of nucleic acid detection is simplified. However, most microfluidic chips currentl...

Claims

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