Multi-channel microfluidic chip device for vacuum negative pressure injection, preparation method and application

A microfluidic chip and vacuum negative pressure technology, applied in chemical instruments and methods, laboratory containers, laboratory utensils, etc., can solve the complex manufacturing process, unsuitable for instant diagnosis applications, insufficient accurate quantitative sensing, etc. problem, to achieve the effect of less sample consumption, high-throughput accurate quantitative sensing, and accurate quantitative sensing

Active Publication Date: 2019-07-19
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the above methods are limited by the complexity of the production process, the need for dilution of whole blood, and the need for pumps or other equipment to drive fluid manipulation, so they are not suitable for point-of-care diagnostic applications.
In addition, existing methods are deficient in plasma separation and subsequent analysis and detection docking, as well as accurate quantitative sensing of samples.

Method used

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  • Multi-channel microfluidic chip device for vacuum negative pressure injection, preparation method and application
  • Multi-channel microfluidic chip device for vacuum negative pressure injection, preparation method and application
  • Multi-channel microfluidic chip device for vacuum negative pressure injection, preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] In this embodiment, the multi-channel microfluidic chip detection device for vacuum negative pressure sampling and blood filtration is composed of a microfluidic chip and a microporous filter membrane. Such as figure 1 As shown, the microfluidic chip is a polydimethylsiloxane (PDMS) microfluidic chip, which is formed by bonding a thin layer of PDMS engraved with a microchannel structure and a thin layer of PDMS without microchannels.

[0053] The microchannel structure in the PDMS thin layer is a main channel and eight parallel loading channels connected vertically. The structure of the entire microchannel is similar to the shape of a comb, such as figure 2 Shown.

[0054] In this embodiment, the eight sample loading channels are located on the same side of the main channel, and the channel width and the distance between the channels are set to be the same. The solution enters from the main channel port 1 and then disperses into eight tributaries into the sample loading chan...

Embodiment 2

[0058] The preparation method of the multi-channel microfluidic chip device with vacuum negative pressure sampling in the present invention includes the following steps:

[0059] (1) For the production of PDMS microfluidic chip, see Figure 4 , The specific production process can be divided into the following steps:

[0060] (1-1) Mask design and production: design the mask pattern of the microfluidic chip, and print the mask pattern on the film through a printer (resolution greater than 20000dpi) to obtain the film mask.

[0061] (1-2) Substrate cleaning and homogenization: After the substrate is strictly cleaned with acetone, Piranha solution and ultrapure water and dried at high temperature (150℃×1h), a proper amount of SU-8 photoresist is added to the center of the substrate. (About 1ml / inch 2 ), placed on the turntable of the glue homogenizer, and homogenized the glue using a program of 600rpm×18s, 2100rpm×60s (increasing the speed at a speed of 100rpm / min). The whole process o...

Embodiment 3

[0078] A multi-channel microfluidic chip detection device with vacuum negative pressure sampling and blood filtration. The microfluidic chip is formed by bonding a thin layer of polydimethylsiloxane (PDMS) engraved with microchannels and a thin layer of PDMS without microchannels. Make a cross cut on the two bonded PDMS to expose the main channel of the microchannel, apply a layer of glue at a certain distance around the exposed main channel opening, and glue a small piece of BTS film on the glue to make the main channel opening It is sealed, thus constituting the final chip device.

[0079] The microchannel structure is composed of one inlet channel and eight outlet channels connected, and its structural features are as follows: the solution inlet channel (hereinafter referred to as the main channel) is a straight channel connected to eight solution outlet channels (hereinafter referred to as the loading channel). The eight sample loading channels are arranged in parallel, with...

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Abstract

The invention relates to a multi-channel microfluidic chip detection device for vacuum negative pressure sample injection and a preparation method and application. The multi-channel microfluidic chipcomprises a main channel, a sample loading channel, a filter membrane, an optical transparent layer etched with a microchannel, and an optical transparent layer not etched with the microchannel; theoptical transparent layer etched with the microchannel is bonded and connected with the optical transparent layer not etched with the microchannel; the main channel and the sample loading channel arein negative pressure states; the filter membrane is located at the upper part of the main channel and bonded with the optical transparent layer etched with the microchannel and the optical transparentlayer not etched with the microchannel. The detection device disclosed by the invention has a small amount of samples and can realize automatic sample injection of the samples, 100% separation of blood cells and plasma of a whole blood sample, and seamless docking between the sample loading channel and a paper chip, so that separation and detection steps can be completed simultaneously.

Description

Technical field [0001] The invention relates to a multi-channel microfluidic chip detection device for vacuum negative pressure sampling and blood filtration. It integrates blood cell filtration of whole blood samples, accurate sample loading, and provides a seamless docking interface for multi-channel paper chips, constructing a key blood sample pretreatment device, which has the potential to be widely used in the field of medical devices. Background technique [0002] Blood is the basic biological material component of the human body, composed of plasma and blood cells. Plasma contains various nutrients such as plasma proteins (albumin, globulin and fibrinogen), lipoproteins, as well as inorganic salts, oxygen, hormones, enzymes, antibodies and cellular metabolites. Blood cells are composed of red blood cells, white blood cells and platelets. Physiological and pathological changes of the body often cause changes in blood components, so the detection of blood components has im...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00
CPCB01L3/502707B01L3/502761B01L2200/12
Inventor 冯晓均马朋刘笔锋
Owner HUAZHONG UNIV OF SCI & TECH
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