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Application of bioactive peptides extracted from human hair in preparation of therapeutic drug for improving protein metabolism of chronic kidney disease, and drug

A technology for chronic kidney disease and bioactive peptides, which can be applied to peptide/protein components, metabolic diseases, drug combinations, etc., and can solve problems such as expensive contracts

Active Publication Date: 2021-08-27
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the contract is expensive and unaffordable for ordinary families

Method used

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  • Application of bioactive peptides extracted from human hair in preparation of therapeutic drug for improving protein metabolism of chronic kidney disease, and drug
  • Application of bioactive peptides extracted from human hair in preparation of therapeutic drug for improving protein metabolism of chronic kidney disease, and drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Quantitatively weigh the washed, degreased and dried human hair, add 7 times of 1.0mol / L C 4 h 10 o 2 S 2 , treated at 90°C for 0.7 hours; the remaining C in the product after the reaction was removed by dialysis 4 h 10 o 2 S 2 , dry at 80±10°C;

[0023] (2) Add 9 times of 8mol / L NH to the product obtained in step 1 2 CONH 2 , treated at 60°C for 18 hours; use dialysis to remove the remaining NH in the product after the reaction 2 CONH 2 , dry at 80±10°C;

[0024] (3) Add 9 times of 0.6mol / L CH to the product obtained in step 2 5 N 3 HCl, treated at 50°C for 1.0 hour; use dialysis to remove the remaining CH in the product after the reaction 5 N 3 HCl, dry at 80±10°C;

[0025] (4) Add 8 times of 1.0mol / L NaOH to the product obtained in step 3, treat at 90°C for 1.0 hour, adjust the pH to 7.4 with HCl; adjust the BAPs content to 50mg / mL by spectrophotometry through dilution, and add NaCl, the osmotic pressure was adjusted to 300±20mOsm / L with an osmome...

Embodiment 2

[0028] (1) Quantitatively weigh washed, degreased, and dried human hair, add 8 times 2.0mol / L C 2 h 6 OS, treated at 80 °C for 0.8 h; the remaining C in the reaction product was removed by dialysis 2 h 6 OS, dry at 80±10℃;

[0029] (2) Add 10 times of 8mol / L NH to the product obtained in step 1 2 CONH 2, treated at 70°C for 10 hours; use dialysis to remove the remaining NH in the product after the reaction 2 CONH 2 , dry at 80±10°C;

[0030] (3) Add 8 times of 0.9mol / L CH to the product obtained in step 2 5 N 3 HCl, treated at 60°C for 1.5 hours; use dialysis to remove the remaining CH in the product after the reaction 5 N 3 HCl, dry at 80±10°C;

[0031] (4) Add 9 times of 2.4mol / L NaOH to the product obtained in step 3, treat at 50°C for 3 hours, adjust the pH to 7.4 with HCl; through concentration, adjust the BAPs content to 100mg / mL by spectrophotometry. Through dialysis, the osmotic pressure was adjusted to 60±10mOsm / L with an osmometer.

[0032] (5) The produ...

Embodiment 3

[0035] Observation on rat CKD nutritional therapy with liquid phase BAPs prepared in Example 1

[0036] Thirty-six male SD rats (SPF grade), weighing 190-210 g, were randomly divided into 3 groups after adaptive feeding for 1 week: normal control group, CKD model group, and BAPs treatment group, with 12 rats in each group. Rats in the normal control group were fed with common feed, drank water freely, and fed 1 mL of normal saline every day. Rats in the CKD model group were fed with 0.75% adenine feed, drank water freely, and fed 1 mL of normal saline every day. Rats in the BAPs treatment group were fed with 0.75% adenine feed, drank water freely, and fed about 1 mL of the liquid-phase BAPs prepared in Example 1 every day (the exact amount of BAPs was 250 mg / kg·d according to the weight of the rats). During the experiment, 2 rats in the CKD model group died. After 6 weeks, blood urea nitrogen (BUN), creatinine (Scr), Alb, PAb and TRF levels in the blood of rats in each group...

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Abstract

The invention discloses an application of bioactive peptides extracted from human hair in the preparation of a therapeutic drug for improving protein metabolism of chronic kidney diseases, and a drug. The human hair extracted BAPs can effectively improve the indexes of serum albumin (Alb), prealbumin (PAb) and transferrin (TRF) of CKD patients, improves the symptoms of protein metabolism disorder caused by renal dysfunction, avoids adverse consequences caused by malnutrition of CKD patients, and delays the progress of CKD to a certain extent.

Description

technical field [0001] The invention specifically relates to the application of a bioactive peptide extracted from human hair in the preparation of a drug for improving protein metabolism of chronic kidney disease and the drug. Background technique [0002] Chronic kidney disease (chronic kidney disease, CKD) prevalence continues to increase, and it has become a global public health problem in the late 20th century. With the development of CKD, patients will develop a series of symptoms and metabolic disorders, and will also be in a state of overall malnutrition. Malnutrition is the main reason leading to higher hospital infection rate and mortality rate in patients with maintenance hemodialysis, which seriously affects their prognosis and quality of life. The cause of malnutrition is that renal dysfunction leads to protein metabolism disorder, which increases protein catabolism, reduces protein synthesis and metabolism, and the body is in a negative nitrogen balance. Sinc...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61P13/12A61P3/02
CPCA61K38/012A61P13/12A61P3/02Y02A50/30
Inventor 肖晖
Owner NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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