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Use of laurus nobilis extract fractions to protect against air pollution related diseases

A technology of air pollution and extracts, which is applied in the direction of respiratory diseases, cardiovascular diseases, and the function of food ingredients, and can solve problems such as protection, damage, and release of pro-inflammatory molecules

Inactive Publication Date: 2021-09-03
DSM IP ASSETS BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Interleukin 8 (IL-8) is part of the innate immune system and is important in the initiation of the immune response, but overstimulation and the resulting dysfunction of neutrophils recruited within the airways can lead to proinflammatory molecules released, causing damage instead of protecting lung tissue

Method used

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  • Use of laurus nobilis extract fractions to protect against air pollution related diseases
  • Use of laurus nobilis extract fractions to protect against air pollution related diseases
  • Use of laurus nobilis extract fractions to protect against air pollution related diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Activation of the Nrf2 pathway

[0078] method:

[0079] Luciferase reporter gene assay using H4IIE-ARE8L cells:

[0080] H4IIE-ARE8L cells are a rat hepatoma cell line stably transfected with a luciferase reporter gene under the control of an eight-repeat antioxidant response element (ARE) (Kratschmar DV et al., 2012. PloS One.2012 ;7(5):e36774).

[0081] The culture medium for H4IIE-ARE8L cells was high glucose Duchenne's modified Eagle's medium (DMEM) containing heat-inactivated 10% fetal bovine serum (FBS). Change the medium every two to three days. As the DMEM assay medium, charcoal-treated FBS (DMEMct) was used.

[0082] Perform transactivation assays in 96-well plates. Plates were seeded with approximately 40'000 cells in 100 μl DMEMct per well and incubated overnight at 37°C. Test compounds were then diluted in DMEDct and administered to cells as described below. Incubate the cells at 37 °C and 5% CO 2 Incubate for at least another 16 h. Equilibrate cel...

Embodiment 2

[0092] Reduced Diesel Particle-Induced Inflammatory Markers

[0093] method:

[0094] The human bronchial epithelial cell line BEAS-2B was obtained from ATCC (American Type Culture Collection, Manassas, VA) and was published in Surface plastic flasks (Corning Inc., Corning, NY) were cultured in bronchial epithelial cell growth medium (BEGM, Lonza, Wakersville, MD). Adenocarcinoma human alveolar basal epithelial A549 cell line was obtained from ATCC and cultured in Kaighn's modified Ham's F-12 medium (F-12K medium) (Life Technologies, USA) supplemented with 10% FBS (Sigma, Saint-Louis, MO). ) cultured. These cells were incubated in 5% CO 2 cultured at 37°C in a humidified atmosphere.

[0095] BEAS-2B cells in 3 to 4 × 10 5 cells / well seeded into 12 wells Surface culture plates (Corning Inc.). A549 cells were treated with 2×10 5 cells / well were seeded in a 12-well plate.

[0096] Diesel particulate matter (Standard Reference Material SRM 1650b, National Institute of...

Embodiment 3

[0119] Identification of active ingredients.

[0120] Four compounds were isolated from the positive sample as discussed at the end of this example and analyzed by NMR spectroscopy to elucidate the structure. The result looks like this:

[0121]

[0122] The spectrum is given as follows:

[0123] LN-3.12: (Formula 1)

[0124]

[0125] A numbering system is used for the assignments in Table 6 below.

[0126] Table 6. Characteristics of the structure of formula 1 1 H and 13 C distribution.

[0127]

[0128] The stereochemistry of this structure could not be determined due to overlapping signals from the protons in C4, C5 and C9.

[0129] LN-3.14: (Formula 2)

[0130]

[0131] Formula 1. The numbering system is used for the assignments in Table 7 below.

[0132] Table 7. Characteristics of the structure of formula 2 1 H and 13 C distribution:

[0133]

[0134] LN-3.16 and LN-3.19: (Formula 3)

[0135]

[0136] Equation 3. The numbering system i...

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Abstract

The present invention relates to systemic detoxification and chronic inflammation by using extract fractions of Laurus nobilis, that lead to a decrease of anti-inflammatory markers in human lung cell lines and the activation of the Nrf2 pathway, which is a crucial element in intracellular detoxification pathways, decreases the expression of inflammatory cytokines. Therefore, the extract can be used to reduce the adverse effects of air pollution generally (and especially of particulate air pollution), which includes: cardiovascular problems, respiratory diseases, and chronic inflammation of tissues that come into contact with air borne particles.

Description

Contents of the invention [0001] The present invention relates to systemic detoxification by using Laurus nobilis (laurus nobilis) (laurel leaf, laurel tree) extract fractions. Background technique [0002] Air pollution is associated with morbidity and mortality mainly due to lung disease and cardiovascular disease. [0003] Inflammation is a key process in the development of diseases induced by air-polluted particulate matter. Interleukin 8 (IL-8) is part of the innate immune system and is important in the initiation of the immune response, but overstimulation and the resulting dysfunction of neutrophils recruited within the airways can lead to proinflammatory molecules released, causing damage rather than protection of lung tissue. [0004] Interleukin-6 (IL-6) is secreted by T lymphocytes and macrophages and also helps stimulate the immune response. IL-6 inhibits the effects of tumor necrosis factor alpha (TNF-α) and interleukin-1 (IL-1). It is mainly associated with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/54A23L33/105A61K31/343A61K45/06
CPCA23L33/105A61K31/343A61K36/54A61K31/365A61P9/00A61P11/00A61P29/00A23L33/10A61K2300/00A23V2002/00A23V2250/30A23V2200/30A61K31/05A61K31/07A61K31/202A61K31/355A61K31/593A61K36/81
Inventor 伊戈尔·本迪克贝蒂娜·博兰诺夫帕斯卡尔·福斯·博塞特休伯特·保罗·哈格纳萨列·理查德圭多·华尔
Owner DSM IP ASSETS BV
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