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Application of buerger's lespedeza root extract in preparation of imipenem anti-pseudomonas aeruginosa sensitizer

A technology of Pseudomonas aeruginosa and imipenem, which is applied in the field of medicine and can solve the problems of synergistic antibacterial effect of ginseng without blood and imipenem

Inactive Publication Date: 2021-09-07
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the reports of the prior art, there is no report that blood ginseng synergizes with imipenem for antibacterial and synergistic effects

Method used

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  • Application of buerger's lespedeza root extract in preparation of imipenem anti-pseudomonas aeruginosa sensitizer
  • Application of buerger's lespedeza root extract in preparation of imipenem anti-pseudomonas aeruginosa sensitizer
  • Application of buerger's lespedeza root extract in preparation of imipenem anti-pseudomonas aeruginosa sensitizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Determination of minimum inhibitory concentration (MIC) of blood ginseng extract on Pseudomonas aeruginosa quality control strain ATCC27853 and clinical isolates.

[0021] Use a small powder machine to crush blood ginseng medicinal materials, sieve, soak overnight in 75% ethanol (w / w=1:10), ultrasonically extract 3 times, 30min each time, filter with suction, combine the filtrates and concentrate to obtain crude blood ginseng Extract.

[0022] Using the microwell double dilution method, adjust the bacterial concentration to 1×10 6 CFU / mL, inoculate in a 96-well sterile culture plate, add each drug into the culture wells containing bacteria, sequentially dilute according to the solubility, incubate in a 37°C incubator for 18-24 hours, read the positive and negative control wells, negative The control wells were clear, and the positive control wells were cloudy. The MIC of blood ginseng extract on bacteria is the lowest drug concentration that inhibits the gr...

Embodiment 2

[0026] Example 2: Determination of the minimum inhibitory concentration of imipenem on Pseudomonas aeruginosa quality control strain ATCC27853 and clinical isolates.

[0027] Using the microwell double dilution method, adjust the bacterial concentration to 1×10 6 CFU / mL, inoculate in a 96-well sterile culture plate, add each drug into the culture wells containing bacteria, sequentially dilute according to the solubility, incubate in a 37°C incubator for 18-24 hours, read the positive and negative control wells, negative The control wells were clear, and the positive control wells were cloudy. The MIC of a drug against bacteria is the lowest drug concentration that inhibits the growth of bacteria visible to the naked eye. In this experiment, the antibacterial effect of imipenem on Pseudomonas aeruginosa quality control strain ATCC27853 and 20 clinical isolates was observed. According to the Clinical and Laboratory Standards Institute (CLSI) drug susceptibility criteria, when ...

Embodiment 3

[0031] Example 3: Determination of the minimum inhibitory concentration of ciprofloxacin on Pseudomonas aeruginosa quality control strain ATCC27853 and clinical isolates.

[0032] Using the microwell double dilution method, adjust the bacterial concentration to 1×10 6 CFU / mL, inoculate in a 96-well sterile culture plate, add each drug into the culture wells containing bacteria, sequentially dilute according to the solubility, incubate in a 37°C incubator for 18-24 hours, read the positive and negative control wells, negative The control wells were clear, and the positive control wells were cloudy. The MIC of a drug against bacteria is the lowest drug concentration that inhibits the growth of bacteria visible to the naked eye. In this experiment, the antibacterial effect of ciprofloxacin on Pseudomonas aeruginosa quality control strain ATCC27853 and 20 clinical isolates was observed. According to the Clinical and Laboratory Standards Institute (CLSI) drug susceptibility crite...

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PUM

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Abstract

The invention discloses an application of a buerger's lespedeza root extract in preparation of an imipenem anti-pseudomonas aeruginosa sensitizer, and belongs to the technical field of Chinese herbal medicines. The application particularly relates to the following steps that buerger's lespedeza root is mixed with an organic solvent, extracting is carried out to obtain the buerger's lespedeza root extract, by correspondingly combining with the imipenem with 1024 [mu]g / mL and 512 [mu]g / mL of the buerger's lespedeza root extract according to 1 / 4 MIC and 1 / 8 MIC of different clinical strains, and the result shows that the buerger's lespedeza root extract can increase the sensitivity of pseudomonas aeruginosa to imipenem.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of a blood ginseng extract in the preparation of an imipenem anti-Pseudomonas aeruginosa sensitizer. Background technique [0002] Pseudomonas aeruginosa (PA) is one of the most common opportunistic pathogens causing severe hospital-acquired infections in clinical practice. When the body's resistance is reduced or the immune function is impaired or invasive operations (such as indwelling catheters, ventilator-assisted ventilation) are likely to cause various infections, such as urinary tract infections, respiratory infections, meningitis, burn infections, etc., the following Respiratory tract infections are the most common, including bronchiectasis co-infection, chronic obstructive pulmonary disease co-infection, ventilator-associated pneumonia, etc., and are mainly distributed in intensive care units. [0003] The widespread use and even abuse of antibiotics le...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61K31/407A61P31/04
CPCA61K36/48A61K31/407A61P31/04A61K2236/33A61K2236/39A61K2300/00
Inventor 周红黄雅思王诺妍
Owner ZUNYI MEDICAL UNIVERSITY
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