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Cell-targeted polypeptide for treating bone tumors as well as preparation method and application of cell-targeted polypeptide

A cell-targeting, bone tumor technology, applied in the field of medicine, can solve the problem of incompatibility between safety and effectiveness

Active Publication Date: 2021-09-10
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a cell-targeted polypeptide for the treatment of bone tumors and its preparation method and use, which are used to solve the safety of bone tumor cell-targeted drugs in the prior art The problem of incompatibility with effectiveness

Method used

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  • Cell-targeted polypeptide for treating bone tumors as well as preparation method and application of cell-targeted polypeptide
  • Cell-targeted polypeptide for treating bone tumors as well as preparation method and application of cell-targeted polypeptide
  • Cell-targeted polypeptide for treating bone tumors as well as preparation method and application of cell-targeted polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] The preparation method of polybutamine nanocomposite particles in this embodiment is as follows:

[0061] 9.05mg Mn (COO) 2 · 4h 2 O Soluble in 10 ml of 75% ethanol, then 10 mg of dopamine, dopamine to be hydrochloride, and the polyammine is completely dissolved, and 1M is adjusted to adjust pH = 8.5. The mixed solution was stirred at 30 ° C for 30 min, add 16.6 mgK 3 [Co (CN) 6 The mixed solution was stirred at 30 ° C for 24 h at 30 ° C. The reaction product was centrifuged for 20 min 12000 rpm, and centrifugation was repeatedly washed three times with deionized water, and a polybicine nanocomposite particles were obtained. (Remember to m @P)

[0062] The preparation method of the bone tumor cell targeting drug carrier in this embodiment is as follows:

[0063] The polypeptide and the above-described polybatamine nanocomposite particles were mixed in a molar ratio of 20: 1, and then stirred in aqueous solution of 1 M ammonia water to stir for 24 h, 12000 rpm was centrifuge...

Embodiment 2

[0067] The preparation method of polybutamine nanocomposite particles in this embodiment is as follows:

[0068] 18.1mg Mn (COO) 2 · 4h 2 O Soluble in 10 ml of 75% ethanol, then 5 mg of dopamine, dopamine to be completely dissolved, and 1 M ammonia was used to regulate pH = 8.5. The mixed solution was stirred at 30 ° C for 30 min, add 8.3 mgK 3 [Co (CN) 6 The mixed solution was stirred at 30 ° C for 24 h at 30 ° C. The reaction product was centrifuged for 20 min 12000 rpm, and centrifugation was repeatedly washed three times with deionized water, and a polybicine nanocomposite particles were obtained. (Remember to m @P)

[0069] The preparation method of composite particles targeted in this embodiment is as follows:

[0070] The bone tumor cells target peptides and polybatamine nanocomposite particles were mixed with molar ratio of 100: 1, and then stirred in aqueous solution of 1 M ammonia water to 24 hours, 12000 rpm was centrifuged three times, resulting in polypeptide-modified...

Embodiment 3

[0074] The preparation method of the polydamine in this example is as follows:

[0075] 40 mg of dopamine is dissolved in 10 ml of 75% ethanol, and the dopamine to be hydrochloride is completely dissolved, and 1M is adjusted to adjust pH = 8.5. The solution was stirred at 30 ° C magnetic for 24 h. The reaction product was centrifuged for 20 min, and the centrifugation was repeatedly washed three times with deionized water, i.e., obtaining a polybicine nanocomposite particles (remembient PDA).

[0076] The preparation method of the bone tumor cell targeting drug carrier in this embodiment is as follows:

[0077] The bone tumor cells target peptides and polyopaline were mixed in molar ratio (20: 1), and then stirred in aqueous solution of 1 M ammonia water to stir for 24 h, 12000 rpm was centrifuged three times, resulting in bone tumor cell targeted peptide modification Dopamine nanocomposite particles (recorded as TP).

[0078] The preparation method of bone tumor cell targeting ph...

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Abstract

The invention belongs to the field of research and development of biological medicines, and particularly relates to a cell-targeted polypeptide for treating bone tumors as well as a preparation method and application of the cell-targeted polypeptide. The polypeptide has a structure as shown in a formula I; the formula I is Dm-X-Rn-C, m is the number of aspartic acid, and m is larger than or equal to 6 and smaller than or equal to 10; n is the number of arginine, and n is larger than or equal to 6 and smaller than or equal to 10; X is a polypeptide fragment which can be recognized and sheared by MMP. The polypeptide-modified polydopamine nanoparticles taking the metal organic framework as the core are loaded with an anti-tumor drug adriamycin, so that the targeting effect can be obviously enhanced, and the toxic and side effects of the drug can be reduced.

Description

Technical field [0001] The present invention relates to the field of medical technology, and more particularly to a cell targeting polypeptide for treating a bone tumor and a preparation method thereof. Background technique [0002] Malignant bone tumors are a common and difficult to cure tumor, which is still a big problem. Surgery and chemotherapy are the main method of clinical treatment of bone tumors. Surgery can extend the life cycle of bone tumor patients, but the surgical edge or small lesion may cause tumor recurrence. Chemotherapy is a subsequent postoperative adjuvant treatment for bone tumors. Clinical chemotherapy drugs typically use systemic administration, lack targeting, so that effective treatment concentrations can not be achieved at the bone tumor, resulting in poor chemotherapy. In addition, chemotherapy side effects are severe, which is easy to generate drug resistance. The bone micro environments provide fertile soil for raising, survival and development of ...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K7/08A61K47/62A61K47/69A61K31/704A61K41/00A61P35/00A61P35/04
CPCC07K7/06C07K7/08A61K47/62A61K47/6929A61K31/704A61K41/0052A61P35/00A61P35/04A61K2300/00
Inventor 林开利王仡桐崔金婕王旭东张雷
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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