Epidermal stem cell amplification culture medium as well as preparation method and application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of epidermal stem cells and medium, applied in the field of stem cells, can solve the problems of fast cell differentiation, limited cell number, poor stability, etc.
Inactive Publication Date: 2021-09-10
郑州广毓生物科技有限公司
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0004] There are many problems in the in vitro expansion of existing epidermal stem cells, such as the complex composition of the medium, the need for trophoblast co-cultivation, poor stability, etc., there are safety hazards in clinical application, and the cell proliferation rate is slow, and a sufficient number cannot be obtained in a short time Epidermal stem cells, etc., cells differentiate quickly, and generally can only be passaged to P3-P5 generations, which also limits the number of cells that can be expanded.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0027] A medium for epidermal stem cell expansion, consisting of basal medium RPMI1640 and the following components added to RPMI1640 medium: thymosin α1, glycerol phosphatidylcholine, raffinose, ferrous glycinate, coenzyme Q10, vitamin E, and compound amino acids ;The final concentration of each component in the medium is: thymosin α1 2.8μg / mL, glycerol phosphatidylcholine 2.4μg / mL, raffinose 3.2ng / mL, ferrous glycinate 12.5ng / mL, coenzyme Q10 2.5ng / mL mL, vitamin E 15 μg / mL, glutamic acid 6 μg / mL.
[0028] The preparation method of the above-mentioned medium comprises the following steps: adding thymosin α1, glycerol phosphatidylcholine, raffinose, ferrous glycinate, coenzyme Q10, vitamin E, and compound amino acids to the basal medium, stirring evenly, and using a 0.22 μm filter to Sterilize by membrane filtration.
Embodiment 2
[0030] A medium for epidermal stem cell expansion, consisting of basal medium RPMI1640 and the following components added to RPMI1640 medium: thymosin α1, glycerol phosphatidylcholine, raffinose, ferrous glycinate, coenzyme Q10, vitamin E, and compound amino acids ;The final concentration of each component in the culture medium is: thymosin α1 2.5μg / mL, glycerol phosphatidylcholine 1.8μg / mL, raffinose 3.0ng / mL, ferrous glycinate 10ng / mL, coenzyme Q10 1.5ng / mL , vitamin E12μg / mL, arginine 2μg / mL, tryptophan 3μg / mL.
[0031] The preparation method of the above medium is the same as in Example 1.
Embodiment 3
[0033] A medium for epidermal stem cell expansion, consisting of basal medium RPMI1640 and the following components added to RPMI1640 medium: thymosin α1, glycerol phosphatidylcholine, raffinose, ferrous glycinate, coenzyme Q10, vitamin E, and compound amino acids ; The final concentration of each component in the medium is: thymosin α1 3.2 μg / mL, glycerol phosphatidylcholine 2.7 μg / mL, raffinose 5.5 ng / mL, ferrous glycinate 15 ng / mL, coenzyme Q10 3 ng / mL, Vitamin E 18μg / mL, lysine 8μg / mL.
[0034] The preparation method of the above medium is the same as in Example 1.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses an epidermal stem cell amplification culture medium. The epidermal stem cell amplification culture medium comprises a basic culture medium and the following components added in the basic culture medium: thymosin alpha 1, glycerol phosphatidylcholine, raffinose, ferrous glycinate, coenzyme Q10, vitamin E and compound amino acid. According to the culture medium, trophoblasts do not need to be added for co-culture, the proliferation rate of epidermal stem cells can be remarkably increased by adding thymosin alpha 1 and glycerol phosphatidylcholine, and the number of cells obtained through culture is increased. And raffinose and ferrous glycinate are also added into the culture medium, so that the epidermal stem cells can maintain an undifferentiated state, the proliferation activity of the cells is maintained, and the subculture efficiency of the cells is improved. The invention further provides a preparation method of the epidermal stem cell amplification culture medium, and the preparation method is simple and convenient in process and easy to operate. The invention further provides application of the culture medium in epidermal stem cell amplification, in-vitro mass amplification of epidermal stem cells is achieved, and a large number of epidermal stem cells can be harvested within a short time.
Description
technical field [0001] The invention relates to the field of stem cells, in particular to an epidermal stem cell expansion medium, a preparation method and application thereof. Background technique [0002] The skin is the largest organ that constitutes the human body. It is in direct contact with the external environment, and it protects the human body from various harmful factors including harmful microorganisms and external stimuli such as ultraviolet rays. An organ essential for the maintenance of required biochemical functions. There are many kinds of stem cells in the skin epidermis, among which epidermal stem cells exist in the outermost basal layer of the skin. It plays an important role in the formation of epidermis, the stability of epidermal tissue structure, the healing of skin wounds, and the occurrence and development of various skin diseases. [0003] With the development of biology, it is found that epidermal stem cells play an extremely important role in t...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more