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Application of tectorigenin in preparation of Listeria monocytogenes sortase A inhibitor

A technology of Listeria monocytogenes and irisin, applied in the directions of plant/algae/fungus/moss ingredients, antibacterial drugs, organic active ingredients, etc.

Inactive Publication Date: 2021-09-17
JILIN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, there are no reports at home and abroad that irisaxanthin can resist Listeria infection by inhibiting the catalytic activity of Listeria monocytogenes sortase A

Method used

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  • Application of tectorigenin in preparation of Listeria monocytogenes sortase A inhibitor
  • Application of tectorigenin in preparation of Listeria monocytogenes sortase A inhibitor
  • Application of tectorigenin in preparation of Listeria monocytogenes sortase A inhibitor

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Embodiment Construction

[0012] Further describe the present invention by way of example by following implementation, do not limit the present invention in any way, under the premise of not departing from the technical solution of the present invention, any modification or change that the person of ordinary skill in the art that the present invention is done to realize easily will fall into within the scope of the claims of the present invention.

[0013] 1. Effect of irisaxanthin on SrtA enzyme activity

[0014] In this study, the inhibitory effect of irisaxanthin on SrtA enzyme activity was detected by fluorescence resonance energy transfer experiment (FRET). The polypeptide sequence recognized and cut by Listeria monocytogenes SrtA is LPETG, and the substrate polypeptide used in this experiment is Dabcyl-QALPETGEE-Edans. After co-incubating SrtA protein with different concentrations (0-16 μg / mL) irisaxanthin in a 96-well black plate at 37°C for 30 min, add a fluorescently modified substrate peptid...

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Abstract

The invention relates to application of tectorigenin in preparation of a Listeria monocytogenes sortase A inhibitor. SrtA enzyme activity experiments, biofilm experiments and cell invasion experiments prove that tectorigenin can effectively inhibit SrtA catalytic activity and has a certain inhibition effect on the pathogenicity of Listeria monocytogenes; and a mouse listeria monocytogenes systemic infection model treatment experiment proves that tectorigenin has a good treatment effect on infection caused by listeria monocytogenes.

Description

technical field [0001] The invention relates to the application of irisxanthin in inhibiting the catalytic activity of Listeria monocytogenes sortase A and preparing Listeria infection drugs, and belongs to the technical field of medical pharmacy. Background technique [0002] Listeria monocytogenes (Listeria monocytogenes for short) is an important Gram-positive opportunistic pathogenic bacteria in veterinary clinics, and it is also an important model pathogen for the research and evaluation of antibacterial infection drugs. Sortase A (SrtA) plays a key role in the virulence of the pathogenic bacteria in the process of Listeria monocytogenes infection, and is an important potential target for the development of anti-infective drugs for Listeria monocytogenes. Antibiotics are the first choice for the treatment of bacterial infections. With their long-term, large-scale and irrational use in medical and animal husbandry, the problem of bacterial resistance is becoming more and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K36/88A61P31/04
CPCA61K31/352A61K36/88A61P31/04
Inventor 王建锋张思琪刘敏达周永林沈雪王琳冯海华邓旭明
Owner JILIN UNIV
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