Method for inducing mouse pruritus cutanea model using dermatophagoides pteronyssinus protein and SEB

Abstract

The invention provides a method for inducing a mouse pruritus cutanea model by using dermatophagoides pteronyssinus protein and SEB, which comprises the following steps: adaptively feeding a mouse for 7 days, anesthetizing the mouse with isoflurane, shaving the back, smearing depilatory paste, scraping the depilatory paste and hair after 10 minutes, forming a 3-5cm < 2 > depilatory area, slightly scraping the depilated skin to cause skin lesion, and the like. According to the method, a mixed allergen is adopted to sensitize the local skin of the mouse, the total time of model preparation and model preliminary verification is 40 days, the model making period is shorter than that of 7 weeks of sensitization of a single allergen, and the model making severity is deeper. The mouse strain is common C57BL / 6 mouse, the price is low, and the model forming rate is 70% or above.

Description

technical field [0001] The invention belongs to the technical field of animal experiment model construction and relates to a method for inducing a mouse skin pruritus model by using house dust mite protein and SEB. Background technique [0002] Pruritus (atopic dermatitis) is a chronic skin disease without primary skin lesions. It can be stimulated by certain diseases, drugs, cold, and allergens. It is a neurological dysfunction skin disease, and it is still a refractory skin disease. Clinically, the patient feels severe itching without primary skin lesions. The site of occurrence is not fixed and can be generalized or limited to certain parts such as: head and neck, trunk, limbs, and vagina. Scratch marks, papules, and blood scabs can occur after itching , skin thickening and lichenification changes, the patient is itching unbearably, suffering unbearably. [0003] The current common model animals for atopic dermatitis include rabbits, rats, mice, guinea pigs, dogs, etc. ...

AI Technical Summary

Problems solved by technology

[0004] Spontaneous animal models include Nc / Nga mice, which are characterized by severe erythema, itching, dry skin, skin barrier dysfunction and other symptoms in ordinary environments, and the pathological manifestations are close to the clinical manifestations of human atopic dermatitis. The disadvantage is that it is expensive, limit its wide application

Gene-edited model mice are also expensive and inconvenient to operate, which limits their wide application

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