35 results about "Dermatophagoide" patented technology

Isolated nucleic acids encoding an allergen of Dermatophagoides pteronyssinus, Der p III, are disclosed. A cDNA encoding a peptide having a Der p III activity and a predicted molecular weight of about 24,985 daltons is also described. The nucleic acids can be used as probes to detect the presence of Der p III nucleic acid in a sample or for the recombinant production of peptides having an activity of Der p III. Peptides having an activity of Der p III can be used in compositions suitable for pharmaceutical administration or methods of diagnosing sensitivity to house dust mites.

The invention provides a method for preparing an allergen diagnostic reagent of acute allergic diseases caused by dermatophagoides pteronyssinus (Der p for short). The allergen diagnostic reagent is Der p allergen pricking liquid. The invention also provides a method for preparing an allergen diagnostic reagent raw material, namely Der p pure mite allergen, a method for evaluating the valence of the allergen diagnostic reagent and a purpose for specifically diagnosing the allergic diseases. The allergen diagnostic reagent achieves the aim for detecting whether a patient is allergic to a matter to cause a disease by a sensitized principle. The Der p pure mite allergen raw material has short culture period and high purity, and the pricking liquid made of the raw material has high activity, thereby greatly reducing a skin test risk of the allergic patient and improving the safety of clinical medical treatment.

The present invention discloses method of culturing and collecting Dermatophagoides pterronyssinus (Der p), extracting Der p sensibiligen, and measuring the content of the first Der p sensibiligen component (Der p1) inside Der p extracting liquid. The measurement of Der p1 inside Der p extracting liquid is in double-sandwich monoclonal antibody process. In addition, the present invention also discloses the establishment of standard serum library of allergic Der p patient and the test of the total sensibiligen bioactivity of Der p extracting liquid. The method of the present invention has the features of easy operation and fast speed.

The invention belongs to the field of biotechnology, relating to a humanized monoclonal antibody IgG Fab fragment of dermatophagoides farinae 2 allergoid specificity. In the invention, by constructing a humanized immunoglobulin G gene library through a clone blotting method, an ELISA (Enzyme-Linked Immuno Sorbent Assay) sifts the humanized monoclonal antibody IgG Fab fragment of the dermatophagoides farinae 2 allergoid specificity from the library, through expression, purification and identification, the humanized monoclonal antibody IgG Fab fragment is proved to identify the dermatophagoidesfarinae 2 allergoid specificity and have higher affinity therewith and identify the specificity of dermatophagoides farinae crude antigen at the same time. A mast cell degranulation inhibition test shows that the fragment obviously inhibits the combination of dermatophagoides farinae allergen and the allergic disease patient serum IgE attached to the mast cell surface and plays the role of competitively inhibiting antigen combination. Early prevention can be carried out on allergic asthma patients through the Fab antibody fragment, and a medicament composition for preventing and treating the allergic asthma is further prepared.

The invention belongs to biotechnics in particular a cultivation method of dust mite of room. The steps of said method comprises: collection dust with mites, little cultivation in early stage, a vast amount of cultivation in mast bottle by collecting mites with fine needle and repeat collection by saturated saline flotation method, to cultivate dust mites of room in controlled temperature and humidity, to reach effective performance. The invention avoids a lot amount of work and time, pollution when cultivating in open container, low production of mites, and the degree of purity is more than 90 % contrasted to low degree of purity by traditional method; and the cultivation base is easy to attained and in rational proportion.