Dermatophagoides culturing method

Abstract

The invention belongs to biotechnics in particular a cultivation method of dust mite of room. The steps of said method comprises: collection dust with mites, little cultivation in early stage, a vast amount of cultivation in mast bottle by collecting mites with fine needle and repeat collection by saturated saline flotation method, to cultivate dust mites of room in controlled temperature and humidity, to reach effective performance. The invention avoids a lot amount of work and time, pollution when cultivating in open container, low production of mites, and the degree of purity is more than 90 % contrasted to low degree of purity by traditional method; and the cultivation base is easy to attained and in rational proportion.

Description

technical field

[0001] The invention belongs to the field of biological technology, and relates to a method for cultivating dust mites, in particular to a method for cultivating house dust mites. Background technique

[0002] At present, allergic diseases are becoming more and more serious. Dust mites are important indoor allergens. Among them, house dust mites and dust mites are most closely related to human allergic diseases. As an important allergen species, dust mites can cause a variety of allergic diseases such as allergic asthma, rhinitis, and atopic dermatitis. Epidemiological investigations found that 80% of asthma patients are allergic to dust mites, and the allergic rate of children is higher than that of adults.

[0003] The pure culture of dust mites provides a material basis for laboratory research on the allergenicity of mites or their excretions and the development of vaccines for mite allergic diseases. In addition, the preparations used in desensitization...

Examples

Embodiment 1

[0020] Take the dust from mattresses, straw mats, sofas, etc. for microscopic inspection, and carry out initial small-scale culture after morphological identification. Use hair needles to pick 5-10 pairs of dust mites from the dust collected above to a small size of about 2mm medium. No. glass bottle, the bottom area of ​​the glass bottle is 4cm 2 , height 1cm, after 6 to 8 weeks, it is determined that the growth is good and there is no miscellaneous mites, and they are transferred to large bottles for large-scale feeding.

[0021] The culture medium contains the following components: 1) Yeast powder (Japan Wako Pure Chemical Industry Co., Ltd. / domestic), 2) laboratory animal feed (commercially available), 3) fish meal (commercially available), after the animal feed is ground Pass through a 100-mesh sieve, fish meal pass through a 100-mesh mesh sieve, mouse feed and fish meal are dry-heat sterilized at 120°C for 1 hour respectively, and bottled for later use.

[0022] Before ...

Embodiment 2

[0027] Take the dust from mattresses, straw mats, sofas, etc. for microscopic inspection, and carry out initial small-scale culture after morphological identification. Use hair needles to pick 5-10 pairs of dust mites from the dust collected above to a small size of about 2mm medium. No. glass bottle, the bottom area of ​​the glass bottle is 4cm 2 , height 1cm, after 6 to 8 weeks, it is determined that the growth is good and there is no miscellaneous mites, and they are transferred to large bottles for large-scale feeding.

[0028] The culture medium contains the following components: 1) Yeast powder (Japan Wako Pure Chemical Industry Co., Ltd. / domestic), 2) laboratory animal feed (commercially available), 3) fish meal (commercially available), after the animal feed is ground Pass through a 100-mesh sieve, fish meal pass through a 100-mesh mesh sieve, mouse feed and fish meal are dry-heat sterilized at 120°C for 1 hour respectively, and bottled for later use.

[0029] Before ...

technical field

[0001] The invention belongs to the field of biological technology, and relates to a method for cultivating dust mites, in particular to a method for cultivating house dust mites. Background technique

[0002] At present, allergic diseases are becoming more and more serious. Dust mites are important indoor allergens. Among them, house dust mites and dust mites are most closely related to human allergic diseases. As an important allergen species, dust mites can cause a variety of allergic diseases such as allergic asthma, rhinitis, and atopic dermatitis. Epidemiological investigations found that 80% of asthma patients are allergic to dust mites, and the allergic rate of children is higher than that of adults.

[0003] The pure culture of dust mites provides a material basis for laboratory research on the allergenicity of mites or their excretions and the development of vaccines for mite allergic diseases. In addition, the preparations used in desensitization...

Examples

Embodiment 1

[0020] Take the dust from mattresses, straw mats, sofas, etc. for microscopic inspection, and carry out initial small-scale culture after morphological identification. Use hair needles to pick 5-10 pairs of dust mites from the dust collected above to a small size of about 2mm medium. No. glass bottle, the bottom area of ​​the glass bottle is 4cm 2 , height 1cm, after 6 to 8 weeks, it is determined that the growth is good and there is no miscellaneous mites, and they are transferred to large bottles for large-scale feeding.

[0021] The culture medium contains the following components: 1) Yeast powder (Japan Wako Pure Chemical Industry Co., Ltd. / domestic), 2) laboratory animal feed (commercially available), 3) fish meal (commercially available), after the animal feed is ground Pass through a 100-mesh sieve, fish meal pass through a 100-mesh mesh sieve, mouse feed and fish meal are dry-heat sterilized at 120°C for 1 hour respectively, and bottled for later use.

[0022] Before ...

Embodiment 2

[0027] Take the dust from mattresses, straw mats, sofas, etc. for microscopic inspection, and carry out initial small-scale culture after morphological identification. Use hair needles to pick 5-10 pairs of dust mites from the dust collected above to a small size of about 2mm medium. No. glass bottle, the bottom area of ​​the glass bottle is 4cm 2 , height 1cm, after 6 to 8 weeks, it is determined that the growth is good and there is no miscellaneous mites, and they are transferred to large bottles for large-scale feeding.

[0028] The culture medium contains the following components: 1) Yeast powder (Japan Wako Pure Chemical Industry Co., Ltd. / domestic), 2) laboratory animal feed (commercially available), 3) fish meal (commercially available), after the animal feed is ground Pass through a 100-mesh sieve, fish meal pass through a 100-mesh mesh sieve, mouse feed and fish meal are dry-heat sterilized at 120°C for 1 hour respectively, and bottled for later use.

[0029] Before ...