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Recombinant lactococcus lactis cocci of expression modified dust mite allergen XDerp2 and application

A Lactococcus lactis, modified technology, applied in the field of genetic resources engineering, can solve the problems of high cost, low safety, poor batch repeatability, etc., and achieve the effects of low cost, avoiding inflammatory reaction and increasing safety.

Inactive Publication Date: 2014-03-12
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

The chemical synthesis of allergen derivatives in vitro or expression in Escherichia coli, a traditional expression system, has disadvantages such as low safety, high cost, and poor batch repeatability.

Method used

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  • Recombinant lactococcus lactis cocci of expression modified dust mite allergen XDerp2 and application
  • Recombinant lactococcus lactis cocci of expression modified dust mite allergen XDerp2 and application
  • Recombinant lactococcus lactis cocci of expression modified dust mite allergen XDerp2 and application

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Experimental program
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Embodiment Construction

[0031] The present invention will be described in more detail below in conjunction with specific examples.

[0032] 1. According to the genome data of Lactococcus lactis, analyze the parameters such as codon preference and GC content of Lactococcus lactis, and extract the natural gene sequence SEQ ID No.2 (genebank : AF276239.1) to perform gene adjustment to obtain the sequence SEQ ID No.1 of the modified allergen gene xderp2;

[0033] 2. Cloning the modified allergen gene xderp2 into the Lactococcus lactis expression plasmid pNZ8148 (gifted by Professor Zhong Jin of the Chinese Academy of Sciences) to obtain the recombinant plasmid pNZ8148-XD, and then electrotransfer it into the host Lactococcus lactis strain L.lactis NZ9000 , obtained the recombinant Lactococcus lactis strain L.lactis XD (preservation number CGMCC No.8220), which was handed over to the General Microorganism Center of China Microbiological Culture Collection Management Committee for preservation on September...

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Abstract

According to the invention, modified allergen gene xderp2 is obtained through genetical modification to main allergen Derp2 of dermatophagoides pteronyssinus, and recombinant plasmids and recombinant lactococcus lactis cocci XD containing xderp2 (preservation number is CGMCC No.8220) genes are built. Western blot results show that the protein of the modified allergen gene xderp2 can be expressed in recombinant lactococcus lactis cocci; in addition, animal experiment results show that mite anaphylaxis can be restrained obviously by oral administration of recombinant lactococcus lactis cocci of expression modified dust mite allergen XDerp2.

Description

technical field [0001] The invention relates to genetic resource engineering, in particular to a recombinant Lactococcus lactis strain L.lactisXD expressing a modified dust mite allergen XDerp2 and its application. Background technique [0002] Allergic asthma is a common and frequently-occurring disease in clinical practice. It is caused by many factors, including pollen, hair, and dust mites. Among them, dust mite is the most important type of allergen, and dozens of allergens have been preliminarily identified; 80%-90% of dust mite allergic patients have allergic reactions to Derp2. Currently, specific immunotherapy (SIT) is considered to be the most effective way to treat allergies, but successful immunotherapy depends on high-quality allergens. The dust mite allergen preparations currently used in clinical treatment are mostly natural allergen mixtures isolated from nature, and there are unavoidable side effects in the treatment of SIT: first, for an allergic patient, ...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12N1/21A61K39/35A61P37/08C12R1/46
CPCA61K39/00C07K14/43531
Inventor 陈卫张秋香艾春青王刚田丰伟刘小鸣赵国忠赵建新张灏郭敏
Owner JIANGNAN UNIV
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