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Segmented soybean deterioration tolerance identification method and application thereof

An identification method and a segmented technology, applied in the field of soybean seed identification, can solve the problems of lack of germination rate of germplasm, difficulty in distinguishing germplasm, single germination rate, etc., to achieve scientific and reasonable identification results, scientific and reasonable identification methods, and satisfying The effect of production demand

Active Publication Date: 2021-10-22
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Previous identification methods for soybean deterioration often set a specific temperature or degree of treatment, and use a single germination rate to measure. If the deterioration conditions are set too strongly, most of the germplasm will not have a germination rate, and it is difficult to distinguish these germplasms. For the germplasm with zero rate, if the deterioration condition is too mild, the germplasm that is not resistant to deterioration can be distinguished, but the germplasm with strong resistance is above 90%, and the germplasm that is resistant to deterioration cannot be distinguished
Therefore, it is difficult for this traditional identification method to effectively distinguish the resistance to deterioration of all germplasms.

Method used

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  • Segmented soybean deterioration tolerance identification method and application thereof
  • Segmented soybean deterioration tolerance identification method and application thereof
  • Segmented soybean deterioration tolerance identification method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0066] Count the germination rate of soybean materials by setting different temperatures, humidity, and treatment days. It can be seen from Table 1 that under the condition of 40°C and the humidity is 95% among several soybean germination treatments, Guichun No. 8 (more resistant to deterioration) and Guixia No. 1 (not resistant to deterioration) need to be treated for a longer period of time. The germination ability is reduced after a long time, and when the humidity is 95%, it is easy to produce mildew and affect the test effect. However, under the condition of 50°C, the comprehensive germination ability of Guixia No. 1, which was not resistant to deterioration, was only reduced to about 60% when the humidity was deteriorated at 10% and 50% for 20 days, while the comprehensive germination ability of Guichun No. 8, which was more resistant to deterioration no significant difference from the control. Humidity 85% and 95% were treated for 5 days, and the germination power of t...

Embodiment 2

[0071] The deterioration resistance of 108 soybean cultivars was identified by using the segmented soybean deterioration identification method. Guixia 1 was selected as the public control material D1, and Gui 1607 was selected as the public control material D2.

[0072] Take the public control material 1 Guixia No. 1 and 140 intact seeds of soybean varieties to be identified for T5 treatment. After the treatment, put the seeds into 75% alcohol to sterilize for 5 seconds, dry them naturally and put them into a germination box (12cm*12cm) covered with 2 layers of germination paper, then add an appropriate amount of pure water to the germination box, and then cover Wet the upper layer of germination paper with pure water, cover the germination box and place it in an environment of 25°C for germination. After 5 days of germination, count the number of normal buds, mildly damaged buds, damaged buds and severely damaged The number of buds, the reference for the identification of th...

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Abstract

The invention relates to the field of soybean seed identification methods, and particularly discloses a segmented soybean deterioration tolerance identification method and application thereof. The identification method comprises the following steps: (1) setting a control group; (2) performing T5 treatment on a to-be-identified material and the public control together; (3) carrying out a germination test; (4) counting the number of normal buds, the number of lightly damaged buds, the number of damaged buds and the number of seriously damaged buds, and calculating a comprehensive germinating power value V=normal bud rate+ 90%*light damage rate+60%*damage rate+20%* serious damage rate; and (5) judging the deterioration resistance grade according to the comprehensive germinating power value V; or adopting other different treatment modes to further judge the deterioration resistance grade. According to the invention, the segmented identification method is adopted, that is, a plurality of treatment points are set for resistance identification, the germinating power of the soybean material under different damage degrees is fully considered, the comprehensive germinating power value is adopted as the basis of the deterioration resistance identification grade, the deterioration resistance of all germplasm can be identified, and the identification method is more scientific and reasonable.

Description

technical field [0001] The invention belongs to the technical field of soybean seed identification methods, in particular to a segmented soybean deterioration tolerance identification method and its application. Background technique [0002] Spring soybeans in the south are faced with high temperature and high humidity conditions when they are mature and after harvesting. The seeds are prone to deterioration and the seed vigor is reduced. In addition, during the storage of soybean seeds at room temperature, the seed vigor will be lost quickly due to the high temperature, which seriously affects the southern region. Development of spring soybeans. Soybean originated in my country, which has abundant soybean germplasm resources, and related studies have shown that there are extremely rich genetic variations in soybean germplasm resources in terms of seed deterioration resistance. Establishing a reasonable identification method for soybean deterioration is of great significanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02A01C1/00
CPCA01C1/025A01C1/00
Inventor 陈文杰宁德娇郭小红梁江陈渊韦清源汤复跃
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI