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CAR vector of specific high-expression protein MSLN for targeing gastric cancer cell as well as construction method and application thereof

A gastric cancer cell, specific technology, applied in the direction of targeting specific cell fusion, anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, DNA/RNA fragments, etc., can solve the problem of unsatisfactory effect. , radiotherapy and chemotherapy, toxic side effects, drug resistance and other problems, to avoid the down-regulation of MHC expression, and to achieve good efficacy and safety.

Inactive Publication Date: 2021-11-05
SHANGHAI YIHAO BIOTECH CO LTD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Surgery is the only possible way to cure gastric cancer, but the simple surgical treatment of advanced gastric cancer may have residual lesions under the naked eye or under the microscope, which greatly increases the recurrence and metastasis rate; radiotherapy and chemotherapy are toxic and side effects, and the curative effect cannot achieve satisfactory results; molecular targeting Treatment such as trastuzumab has drug resistance and no obvious benefit; immunotherapy such as immune checkpoint inhibitor PD-L1 antibody has not observed significant relief in gastric cancer, which may be related to the tumor cells themselves and their microbiome. environment related

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  • CAR vector of specific high-expression protein MSLN for targeing gastric cancer cell as well as construction method and application thereof
  • CAR vector of specific high-expression protein MSLN for targeing gastric cancer cell as well as construction method and application thereof
  • CAR vector of specific high-expression protein MSLN for targeing gastric cancer cell as well as construction method and application thereof

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Embodiment Construction

[0042] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be described in further detail below in conjunction with specific embodiments and with reference to the accompanying drawings.

[0043]The present invention provides a CAR carrier targeting gastric cancer cell-specific high-expression protein MSLN, the specific scheme is as follows: comprising a chimeric antigen receptor and a carrier, the chimeric antigen receptor is connected to the carrier, and the chimeric antigen receptor includes a single chain of MSLN Antibody, MSLN single-chain antibody consists of nucleotide sequences such as SEQ ID NO.2, SEQ ID NO.5, and a flexible Linker. Anti-MSLN is expressed against the tumor-associated antigen MSLN on the surface of gastric cancer cells. Anti-MSLN is responsible for recognizing the highly expressed MSLN in gastric cancer tissues, which greatly increases the targeting of CAR-T cells and reduces tumor i...

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Abstract

The invention belongs to the technical field of immune cells, and particularly relates to a CAR vector of specific high-expression protein MSLN for targeting gastric cancer cell as well as a construction method and application thereof. The CAR vector comprises a gene for encoding a chimeric antigen receptor, and the structure of the CAR is SP-MSLNscFv-TM-CD28-4-1BB-CD3zeta. CAR-T cells containing the vector are obtained in a virus infection mode, and the specific CAR-T cells can recognize and kill gastric cancer cells highly expressing tumor associated antigen MSLN in a targeted mode by expressing the unique CAR structure.

Description

technical field [0001] The invention belongs to the technical field of biomedical immune cells, and in particular relates to a CAR vector targeting gastric cancer cell-specific high-expression protein MSLN and its construction method and application. Background technique [0002] Gastric cancer is one of the most common malignant tumors in clinical practice in my country. It has the characteristics of high morbidity and high mortality. In my country, the incidence and mortality of gastric cancer occupy the third place among malignant tumors. At present, the treatment methods for gastric cancer mainly include surgery, radiotherapy, chemotherapy, targeted therapy, and immunotherapy. Surgery is the only possible way to cure gastric cancer, but the simple surgical treatment of advanced gastric cancer may have residual lesions under the naked eye or under the microscope, which greatly increases the recurrence and metastasis rate; radiotherapy and chemotherapy are toxic and side e...

Claims

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Application Information

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IPC IPC(8): C12N15/867C12N15/62C12N15/66C12N5/10
CPCC12N15/86C12N15/66C07K16/30C07K16/2821C07K14/7051C12N5/0636C12N2740/15043C12N2510/00C07K2317/622C07K2319/02C07K2319/03C07K2319/33
Inventor 田晓丽
Owner SHANGHAI YIHAO BIOTECH CO LTD
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