Integrated detection device and detection method for LAMP or RT-LAMP

Abstract

The invention relates to an integrated detection device and detection method for LAMP or RT-LAMP. The integrated detection device comprises a support, the support comprises a fixed plate and a movable plate, the fixed plate is provided with a row of reaction tubes, and the reaction tubes are filled with amplification reaction reagent freeze-dried powder; supporting pieces are arranged on the bottom face of the fixed plate; a pair of placement parts is arranged on the top surface of the fixed plate, two ends of the movable plate are slidably connected with the placement parts; a row of flushing holes are formed in the movable plate, FTA cards are fixedly arranged in the flushing holes respectively, and first sealing rings are arranged at the bottoms of the flushing holes; the bottom surface of the movable plate is also provided with a row of second sealing rings and a color developing agent component which is arranged in a strip shape; and a color developing agent is arranged in the color developing agent component. According to the detection method, the LAMP or RT-LAMP experiment detection process is implemented based on the above device. The LAMP or RT-LAMP experimental detection process can be smoothly implemented without depending on an expensive automatic detection instrument which is difficult to carry, the structural design is simple and ingenious, and the operation process is simple, convenient and feasible.

Description

technical field [0001] The invention relates to an integrated detection device and detection method for LAMP or RT-LAMP, belonging to the technical field of nucleic acid detection auxiliary devices. Background technique [0002] Some infectious diseases with the same syndrome (such as viral hemorrhagic fever series with fever and hemorrhage, viral eruption series with fever and rash, etc.) have similar main clinical manifestations, and it is difficult to differential diagnosis in the early stage. The final etiology confirmation depends on pathogen detection. Diagnosis is critical to controlling infectious disease outbreaks. Due to the suddenness and rapid spread of infectious disease outbreaks, it is best to carry out rapid pathogen detection on the spot as soon as possible, because patient specimens or insect-borne specimens are transported from the field or in the field to qualified laboratories, which are often far away. It takes a long time, delays treatment and prevent...

AI Technical Summary

Problems solved by technology

[0006] However, there are still bottlenecks in applying the LAMP / RT-LAMP method to the above on-site detection scenarios: First, the method needs to extract nucleic acid, which is cumbersome to operate manually, or needs to be equipped with a more expensive automatic nucleic acid extractor, and the nucleic acid The separation of extraction and detection can easily lead to pollution and false positive problems; second, the method needs to prepare a reaction system, and the degree of integration is not high; third, the related reagents of LAMP / RT-LAMP generally need cold chain storage, which is inconvenient for transportation and storage; fourth, the current LAMP The / RT-LAMP reaction is still difficult to achieve multiple reactions. Generally, it can only detect a single pathogen, and the throughput is insufficient. It is difficult to meet the needs of multiple pathogen screening for the same syndrome (such as fever with bleeding, four screenings for infectious diseases, etc.).

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