Cryopreservation solution and cryopreservation method for immune cells

A technology of immune cells and cryopreservation method is applied in the field of making Miao medicine Qi and moxibustion moxibustion strips, which can solve the problems of easy aging of cells, low cell viability, and easy damage of immune cells by ice crystals, so as to improve the effect of cryopreservation, Simple formula, the effect of unaffected cell proliferation ability

Inactive Publication Date: 2021-11-09
郑州源创基因科技有限公司
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Problems solved by technology

However, in the existing cryopreservation methods of immune cells, only the cryopreservation of stem cells and tumor cells has been solved, while the method of cryopreservation of immune cells has not been well solved.
Due to the cell structure and cell characteristics of immune cells, when using the previous cell freezing solution and

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  • Cryopreservation solution and cryopreservation method for immune cells
  • Cryopreservation solution and cryopreservation method for immune cells

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Embodiment Construction

[0033] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0034] A cryopreservation solution and a cryopreservation method for immune cells of the present invention, comprising FBS, DMSO, 1640 culture solution, low-molecular-weight heparin calcium and antioxidants.

[0035] The volume fraction of the FBS is 10-20.

[0036] The volume fraction of the DMSO is 10-20.

[0037] The volume fraction of the 1640 culture solution is 40-60.

[0038] The volume fraction of the low molecular weight heparin calcium is 8-5.

[...

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Abstract

The invention relates to a cryopreservation solution and a cryopreservation method for immune cells. The cryopreservation solution comprises FBS, DMSO, a 1640 culture solution, low-molecular heparin calcium and an antioxidant. The cryopreservation method of the cryopreservation solution for immune cells comprises the following steps: 1, storing: putting the immune cells into a freezing tube, then adding a freezing medium, slowly blowing and beating, uniformly mixing, and putting the freezing tube into a cooling chamber; 2, cooling for the first time: cooling to 3 DEG C and keeping the temperature for 4 minutes; then cooling to -3 DEG C at a speed of -2 DEG C/min; and finally, reducing the temperature to -40 DEG C at the speed of -11 DEG C/min; 3, raising the temperature to -30 DEG C at the speed of -16 DEG C/min for the first time; 4, cooling for the second time: keeping the temperature at -30 DEG C for 4 minutes; then cooling to -50 DEG C at the speed of -2 DEG C/min; finally, reducing the temperature to -80 DEG C at the speed of -10 DEG C/min; and keeping at -80 DEG C for 5 days; and 5, storing: finally storing in a liquid nitrogen tank for cryopreservation.

Description

technical field [0001] The invention relates to the production of moxa moxibustion strips of Miao medicine, in particular to a freezing solution and freezing method of immune cells. Background technique [0002] Cell cryopreservation is a technology that places cells in a low temperature environment to slow down cell metabolism to achieve long-term storage. The principle is to store the cells at -196°C in liquid nitrogen at ultra-low temperature, which can make the cells temporarily out of the growth state and preserve the cell characteristics, and can be used by resuscitating the cells when needed. Adding a protective agent to the cell suspension during cryopreservation can lower the freezing point of the solution. Under the condition of slow freezing, the water in the cells penetrates out of the cells, reducing the formation of ice crystals in the cells, thereby avoiding damage to the cells during freezing. At present, cell cryopreservation is mainly stored under the ultr...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226A01N1/021A01N1/0284
Inventor 赵辉高萧芳孙宪凯段书娇王艳琦
Owner 郑州源创基因科技有限公司
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