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Infant probiotics-containing human milk freeze-dried powder composition and preparation method and application

A technology of probiotics and freeze-dried powder, applied to bacteria used in food preparation, applications, dairy products, etc., can solve the problems of being vulnerable to the invasion of harmful bacteria, side effects of intestinal flora, and slow establishment

Pending Publication Date: 2021-12-10
河北省生殖医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the technical problems that the establishment of human intestinal flora in the prior art is greatly affected by external factors, easily affected by the invasion of harmful bacteria, slow to establish, and the intestinal flora in a disordered state of antibiotic regulation has great side effects, the present invention Provides a method for preparing a human milk freeze-dried powder composition containing probiotics from infants, using probiotics, human milk freeze-dried powder and prebiotics screened from the intestinal tract of infants as raw materials, the prepared composition can effectively inhibit harmful To prevent the invasion of bacteria, promote the colonization of native strains, and ensure the stable establishment of intestinal flora

Method used

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  • Infant probiotics-containing human milk freeze-dried powder composition and preparation method and application
  • Infant probiotics-containing human milk freeze-dried powder composition and preparation method and application
  • Infant probiotics-containing human milk freeze-dried powder composition and preparation method and application

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preparation example Construction

[0034] In order to obtain a composition that can effectively promote the stable establishment of intestinal flora, promote the colonization of native strains, and regulate the stability of intestinal flora, the application provides a human milk freeze-dried powder composition containing infant probiotics The preparation method: using human milk freeze-dried powder, prebiotics and infant probiotic bacteria powder as raw materials, the preparation method specifically includes the following steps:

[0035] S1: Take the probiotic strains isolated and purified from the intestinal tract of infants, activate and cultivate them for 3 generations, inoculate them into sterile liquid medium for fermentation and culture, centrifuge the obtained probiotic slime from infants, and mix the probiotic slime from infants Mix and emulsify with bacterial powder protective agent, freeze-dry at low temperature to remove water, crush and sieve to get baby probiotic powder;

[0036] S2: Take human mil...

Embodiment 1

[0067] This embodiment provides a human milk freeze-dried powder composition containing probiotics from infants. Bacillus HRHCC001, Bifidobacterium lactis HRHCC002 and Lactobacillus plantarum HRHCC003 are made of bacteria powder as raw materials, and the preparation method of the composition specifically includes the following steps:

[0068] S1: Take Bifidobacterium bifidum HRHCC001, Bifidobacterium lactis HRHCC002 and Lactobacillus plantarum HRHCC003 respectively, activate the strains, and after continuous culture for 3 generations, inoculate them into 120L sterilized liquid culture medium respectively, and inoculate them at 37°C Fermentation under certain conditions, monitor the pH value, OD value and alkali consumption, centrifuge and harvest after reaching the harvest point, and obtain the corresponding probiotics respectively. The formed bacterial powder protective agent is mixed and emulsified at a mass ratio of 1:1.7, placed in a pre-cooled freeze dryer, and freeze-dri...

Embodiment 2

[0080] This embodiment provides 3 kinds of probiotics: Bacteriostatic experiments of Bifidobacterium bifidum HRHCC001, Bifidobacterium lactis HRHCC002 and Lactobacillus plantarum HRHCC003;

[0081] (1): Take Bifidobacterium bifidum HRHCC001, Bifidobacterium lactis HRHCC002 and Lactobacillus plantarum HRHCC003, and mix them separately to obtain 4 groups of samples, among which the samples of the first to third groups are Bifidobacterium bifidum HRHCC001, Bifidobacterium lactis HRHCC001, Bifidobacterium lactis Bacillus HRHCC002 and Lactobacillus plantarum HRHCC003 single strains, the fourth group of samples were Bifidobacterium bifidum HRHCC001 + Bifidobacterium lactis HRHCC002 + Lactobacillus plantarum HRHCC003 mixed bacteria, the four groups of mixed bacteria powder were inoculated in 100ml MRS liquid medium respectively, at 37 Cultivate statically for 48 hours at ℃ to obtain the corresponding probiotic culture;

[0082] (2): get activated escherichia coli, salmonella and stap...

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Abstract

The invention relates to the technical field of foods, in particular to an infant probiotics-containing human milk freeze-dried powder composition and a preparation method and application. Human milk freeze-dried powder, prebiotics and probiotics from infants are used as raw materials, and the selected probiotics are more suitable for an intestinal environment of a human body and have higher colonization ability in an intestinal tract of the human body; the human milk freeze-dried powder prepared by a low-temperature freeze-drying process preserves the activity of active ingredients in human milk to the maximum extent, so that sufficient nutrient substances are supplied while the shelf life of the human milk is extended, which further promotes stable establishment of intestinal flora. The three raw materials cooperate with each other, so that the prepared composition can effectively promote colonization of the intestinal flora, inhibit invasion of harmful bacteria, improve the intestinal microenvironment health of the body and improve the immunity of the body.

Description

technical field [0001] The invention relates to the field of food technology, in particular to a human milk freeze-dried powder composition containing infant probiotics, a preparation method and application thereof. Background technique [0002] Intestinal flora is an important component of the human intestinal micro-ecological system. It can not only synthesize a variety of vitamins necessary for the human body, but also participate in the metabolism of carbohydrates and proteins, and promote the absorption of various mineral elements by the human body. play a vital role in health. [0003] The flora in the human intestinal tract begins to be established at the beginning of life, and it does not develop to a relatively stable level until the age of 4. In the initial stage of the establishment of the intestinal flora in the human body—infancy, it is a key stage for the customization and establishment of the intestinal flora. It provides a solid foundation for the establishm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/152A23C9/156A23C3/00A23C1/08A23L33/10A23L33/135A23L33/21A23L2/52
CPCA23C9/152A23C9/156A23C3/00A23C1/08A23L33/10A23L33/135A23L33/21A23L2/52A23V2002/00A23V2400/517A23V2400/169A23V2400/531A23V2200/30A23V2250/28
Inventor 刘鹏张建瑞周英杰张勇
Owner 河北省生殖医院
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