Fibrous maize-based animal feed with gh30 glucuronoxylan hydrolase

A technology of animal feed and uronic acid wood, applied in animal feed, animal feed, hydrolytic enzymes, etc., can solve the problems of decreased growth ability of broiler chickens and increased sporadic cases

Pending Publication Date: 2021-12-17
NOVOZYMES AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This has led to an increase in sporadic cases of Clostridium perfringens-associated necrotizing enteritis and other enteric diseases, resulting in reduced growth performance of broiler chickens

Method used

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  • Fibrous maize-based animal feed with gh30 glucuronoxylan hydrolase
  • Fibrous maize-based animal feed with gh30 glucuronoxylan hydrolase
  • Fibrous maize-based animal feed with gh30 glucuronoxylan hydrolase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0223] Exogenous glucuronoxylan hydrolases from glycoside hydrolase family 30 (GH30) promote microbial diversity and butyrate production in cecal broiler fermentation of corn fiber in vitro.

[0224] In this in vitro study, the endogenously acting glucuronoxylanase from the glycoside hydrolase (GH) family 30 of SEQ ID NO 1 was tested to dissolve glucurono-arabinoxylan in corn fiber capacity and the subsequent effect of the produced oligosaccharides on the microbiota composition of the caecum of broilers. This enzyme significantly reduced (P<0.01) the insoluble corn fraction. To study the effect of oligosaccharide size on the fermentation profile, oligosaccharides produced from SEQ ID NO 1 were separated and isolated by size exclusion chromatography. Fermentations of pools of fractions with a molecular weight of 4-10 kDa showed higher concentrations of butyrate compared to pools containing oligosaccharides of lower MW (1-4 kDa and 100-500 Da). During in vitro fermentation, bu...

Embodiment 2

[0278] GAX-specific glucuronoxylan hydrolase improves performance and morphological gut health parameters in broiler chickens fed a corn / DDGS / soybean diet.

[0279] Materials and methods

[0280] Chickens were bred and euthanized according to the Belgian Animal Welfare Regulation (2010 / 63 / EC).

[0281] animals and diet

[0282] A total of 480 newly hatched male Ross-308 broilers were randomly divided into 16 pens of 30 chicks per pen, (8 pens per treatment group) and housed on a solid floor covered with wood chips. During the first 4 days of the experiment, the light schedule provided an 18 hour light / 6 hour dark cycle and a 23 hour light / 1 hour dark cycle. Adjust and optimize the room temperature in the stables to the requirements of the birds via the central heating system. All birds were fed the same corn-soybean based mash diet with or without SEQ ID NO 1 supplementation. Diets (Table 1) were formulated to meet the energy and digestible amino acid requirements of b...

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Abstract

Improvement of the intestinal health of a monogastric animal by increasing the levels of cecal butyrate levels in situ in said animal by administering a GH30 glucuronoxylan hydrolase-enriched maize-based animal feed is described. Improving the feed conversion ratio of said animal feed is furthermore observed.

Description

[0001] Sequence Listing Citation [0002] This application contains a Sequence Listing in computer readable form. This computer readable form is incorporated herein by reference. technical field [0003] The present invention relates to improving intestinal health in animals comprising administering corn-based animal feed enriched in GH30 glucuronoxylanase and improving said animal feed by administering GH30 glucuronoxylanase feed conversion ratio. Background technique [0004] Maintaining a healthy gut is important for broiler production and, along with environmental conditions, diet is a key factor affecting microbiota composition. Broiler diets consist primarily of grains and plant protein sources, which contain varying amounts of fiber which, depending on the plant species, can be extremely difficult to digest. [0005] Plant fibers are mainly composed of polysaccharides other than starch, also known as non-starch polysaccharides (NSP). The major NSP in maize is gluc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/14A23K20/163A23K20/189A23K50/30A23K50/75A61K31/715A61K31/716A61K36/899A61P37/04C12N9/24
CPCA23K10/12A23K10/14A23K20/189A23K50/30A23K50/75C12N9/248C12Y302/01136A23K20/163A61K36/899A61P37/04A61P1/14A61K9/0056A61K35/742A61K38/47
Inventor E·A·德拉帕J·拉文N·R·彼泽森
Owner NOVOZYMES AS
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