A kind of preparation method of osteoarthritis marker detection test paper

Abstract

The invention discloses a preparation method of test paper for detection of osteoarthritis markers, which belongs to the technical field of semiconductor biosensor devices; the method first uses CoFe 2 o 4 Composite doping of nanofluid and chromatographic paper to obtain magnetic test paper; thiolation of AuNPs surface to obtain CYS‑AuNPs; uniform doping of CYS‑AuNPs to the above magnetic test paper to obtain surface thiolated magnetic test paper; use EDC / NHS to activate osteoarthritis Antibody solution, modifying antibody molecules on the surface of magnetic test paper by covalent bonding; using bovine serum albumin to block non-specific sites of magnetic test paper to obtain osteoarthritis marker detection test paper; the invention will have excellent electromagnetic properties CoFe 2 o 4 The combination of nanoparticles and paper with excellent mechanical properties is used to detect osteoarthritis markers, which has the advantages of miniature portability, rapidity, low cost, and high sensitivity.

Description

technical field [0001] The invention belongs to the technical field of semiconductor biosensor devices, and in particular relates to a preparation method of a test paper for detecting osteoarthritis markers. Background technique [0002] Osteoarthritis (OA) is a rheumatic disease, a degenerative and irreversible disease, and the most common joint disease in the world. At present, the main diagnostic methods for OA are: X-ray imaging, acoustic emission analysis, magnetic resonance imaging, etc., but most of the above-mentioned methods require expensive large-scale precision instruments, and cannot be effectively diagnosed in the early stage of OA, and the joint space narrows gradually after a few years , indicating that the articular cartilage has begun to thin and has entered the middle and late stages. So there is a need for a method that can diagnose OA at an early stage. [0003] In recent years, some scholars have proposed the use of biomarkers to predict the occurrenc...

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