Novel bifidobacterium longum strain or cosmetic composition comprising same

A technology of bifidobacterium longum and strains, applied in the direction of bifidobacteria, cosmetics, cosmetic preparations, etc., can solve the problems of pigmentation and melanin deposition in multiple places, and achieve the effect of enhancing skin beauty and improving skin health

Pending Publication Date: 2022-01-11
ATOGEN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the physiological function of the skin deteriorates due to excessive synthesis or aging, melanin is deposited into the skin, resulting in spots, freckles, and multiple pigmentation

Method used

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  • Novel bifidobacterium longum strain or cosmetic composition comprising same
  • Novel bifidobacterium longum strain or cosmetic composition comprising same
  • Novel bifidobacterium longum strain or cosmetic composition comprising same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0112]

[0113] Bifidobacterium longum (hereinafter referred to as F5) was isolated from donated feces of newborns (babies born in 2018, Daejeon, Korea). To this end, neonatal feces were diluted with 0.9% (w / v) saline by a 10-fold serial dilution method, and the resulting solution was spread on a bifidobacterium selective (BS, MBcell Seoul, Korea) solid medium and incubated at 37°C. Incubate the bacteria for 48 hours. The bacterial colonies produced in the BS medium were observed with a microscope. By observation, bacteria and bacillus-type bacteria that did not exhibit a catalase reaction were selected and named ATG-F5 strain (hereinafter abbreviated as F5). For the experiments, F5 strains were first cultured in BL or MRS-cys agar solid medium, and then purely isolated colonies were inoculated in broth liquid medium. Microorganisms were incubated overnight (ie for 16 to 20 hours) at 37°C.

Embodiment 2

[0114]

Embodiment 2-1

[0115] Example 2-1. F5 strain 16S rRNA sequencing

[0116] Upon request, Solgent Co., Ltd. (located in Dajeon, Korea) performed 16S rRNA sequence analysis on the F5 strain. Primers used for sequencing analysis were:

[0117] 27F(5'-AGA GTT TGA TCC TGGCTC AG-3'), 518F(5'-CCA GCA GCC GCG GTA ATAC-3'), 907R(5'-CCGTCA ATT CMT TTR AGT TT-3'), 1492R( 5′-GGT TAC CTT GTT ACG ACTT-3′), and the nucleotide sequence was read 4 times in total. Contigs derived from nucleotide sequence alignments of each read were analyzed using the National Center for Biotechnology Information (NCBI) BLAST online tool (https: / / blast.ncbi.nlm.nih.gov / Blast.cgi) Nucleotide sequence.

[0118] The nucleotide sequence of SEQ ID NO:1 obtained by 16S rRNA sequencing (see figure 1 ) were compared with NCBI's BLAST database. As a result, the B. longum strain IRT showed a 99.9% match with the 16S rRNA sequence, thereby indicating that the tested sequence belongs to B. longum when classified by molecular taxonomy...

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Abstract

The present invention relates to a novel Bifidobacterium longum ATG-F5 strain. The F5 strain is a functional beneficial bacterium which is safe from antibiotic resistance, improves skin health through antibacterial activity against the skin pathogen Cutibacterium aches, an antioxidant effect on radicals, skin barrier improvement, and anti-inflammatory effects, and enhances skin beauty through a skin whitening function, a wrinkle improvement function, and a dry skin alleviation function. The strain can be used in a cosmetic composition or health functional food.

Description

technical field [0001] The present invention relates to a new bifidobacterium longum ATG-F5 (Bifidobacterium longum ATG-F5, registration number. KCTC13828BP) strain or a cosmetic composition containing the strain. Background technique [0002] In the field of skin care, a variety of care techniques are being used with their scope of application gradually expanding. Among various technologies, cosmetics with whitening functional substances that brighten the skin, or cosmetics with wrinkle improvement and suppression functions that create youthful-looking skin and promote skin elasticity are preferred. Recently, functional cosmetics that act as a skin barrier and prevent dry skin have also appeared, and due to a shift in people's ideas, whitening and wrinkle-improving cosmetics with more functions are being developed. [0003] Among these technologies, skin whitening cosmetics are based on the principle of inhibition of melanin activity, which determines the color of human sk...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61K8/99A61K35/745A61P17/00A61P17/10A61P17/18A61P29/00A61Q19/00A61Q19/02A61Q19/08C12R1/01
CPCA61P17/18A61P29/00A61P17/10A23L33/135C12N1/205C12N1/20C12R2001/01A61K35/745A61K8/99A61Q19/02A61Q19/00A61Q19/08A61Q19/007A61P17/00A23V2002/00A23V2200/318A23V2400/533
Inventor 李镕贤白宝篮姜智姬郑渡润朴建锡任成薰宋元镐
Owner ATOGEN CO LTD
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