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Nocardioides as well as separation method and application thereof

A technology of Nocardia-like bacteria and Nocardia-like bacteria, which is applied in Nocardia-like bacteria and tobacco flavoring, which can solve the problems of monotonous flavor and efficacy of spices, and improve the smoking quality and aftertaste of cigarettes And the effect of good comfort and improved smoke strength

Active Publication Date: 2022-01-21
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are still few dominant microbial strains that can be used to prepare tobacco flavors, and the aroma and efficacy of the developed flavors are relatively monotonous. Therefore, new aroma-producing microorganisms are continuously screened and developed to meet the needs of tobacco flavors R & D and production needs, with practical necessity

Method used

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  • Nocardioides as well as separation method and application thereof
  • Nocardioides as well as separation method and application thereof
  • Nocardioides as well as separation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] 1. Isolation, cultivation and identification of Nocardioides sp.ST46

[0030] 1.1 Isolation of Nocardia-like bacteria ST46

[0031] Tobacco soil samples were taken from the tobacco planting base in Kunming, Yunnan, sealed in plastic bags, and stored at 4°C for later use. Accurately weigh 10g of soil sample, add 90mL of sterile water, shake on a shaker at 30°C and 180rpm for 30min; dilute the concentration to 10 with sterile water -1 、10 -2 、10 -3 、10 -4 、10 -5 Take 0.2mL of the five concentrations of bacterial solution and spread it on the LB plate medium, set up three parallel experiments for each concentration of the bacterial solution, cultivate it at 30°C for 48 hours, and pick it from the plate of each soil sample For different single colonies, after purification on LB plate medium, 20% of the bacterial volume was added to glycerol, and stored in a -80°C refrigerator for later use.

[0032] The isolated microorganisms were respectively inoculated in LB liquid...

Embodiment 2

[0048] 1. Preparation of Nocardia-like ST46 Fermentation Biological Flavor

[0049] 1.1 Cultivation of Nocardioides sp.ST46

[0050] (1) Test tube slant culture

[0051] Slope preservation medium is used, the medium is R2A agar medium, and the formula of the medium is: 0.5g of glucose, 0.5g of yeast extract, 0.5g of peptone, 0.5g of acid hydrolyzed casein, 0.5g of soluble starch, and 0.3g of sodium pyruvate , dipotassium hydrogen phosphate 0.3g, magnesium sulfate 0.05g, agar 15g, distilled water to 1000mL, pH7.2. Sterilize the culture medium at 121°C for 25 minutes, place it on a slant, inoculate the Nocardia-like strain ST46, and cultivate it at 28°C for 1 week to obtain the strains in the test tube;

[0052] (2) Seed cultivation

[0053] Adopt seed medium, the formula of seed medium is: dextrin 120g, soybean powder 40g, yeast extract 2g, tryptophan 0.5g, β-alanine 5g, magnesium sulfate 0.5g, ammonium phosphate 0.2g, distilled water constant volume to 1000mL, pH7.2. Ster...

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Abstract

The invention discloses nocardioides. The nocardioides is classified and named as nocardioides ST46 in microbiology, and is named as nocardioides sp. in Latin scientific name. The 16S rDNA partial sequence is as shown in a sequence table; and the preservation number of the strain is CGMCC 4.7686. The invention further discloses a culture and separation method of the nocardioides and application of the nocardioides in preparation of tobacco flavor. The nocardioides is a new strain, the tobacco flavor prepared from the nocardioides is used for flavoring cigarettes, the richness of tobacco flavor and the fullness of smoke are remarkably improved, the smoke strength is remarkably improved, and meanwhile, the aftertaste and comfort are good.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to Nocardioid, its separation method and its application in tobacco flavoring. Background technique [0002] Tobacco flavors and fragrances are indispensable additives in cigarette production, and their application is closely related to the establishment of cigarette brands and the formation and development of cigarette styles. However, with the acceleration of the pace of independent flavoring of cigarettes by enterprises, the types, quantity and quality of existing flavors and fragrances can no longer meet the needs of flavoring technicians. Flavor and fragrance researchers develop more flavors and fragrances that can be applied to self-flavoring to meet the actual situation and needs of enterprise flavoring work, which has become a realistic topic for cigarette flavoring and cigarette product research and development. [0003] Using microbial fermentation technology to p...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04A24B3/12C12R1/01
CPCC12N1/20C12P1/04A24B3/12Y02E50/10
Inventor 高莉段焰青刘志华刘秀明李源栋杜刚刘晓飞蒋举兴郭青胡瑞林
Owner CHINA TOBACCO YUNNAN IND
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