28 results about "Nocardia sp" patented technology

The invention discloses a construction method of a nocardia seriolae mycolic acid deficient strain, which belongs to the technical field of aquaculture prevention and control, and comprises the following steps: strain and material preparation, culture and genome extraction, upstream and downstream fragment cloning to construct deletion plasmids, competent cell preparation, electrotransformation and screening, and biological characteristic and toxicity analysis. The nocardia seriola seriola mycolic acid defect type strain is constructed and compared with a wild strain and a back-filling strain for research, the effect of the mycolic acid in the aspects of growth, form, toxicity and the like of thalli can be defined, the relationship between the mycolic acid and the pathogenicity of the thalli can be deeply known, and an important clue is provided for revealing the pathogenic mechanism of the nocardia seriola seriola.

The present invention provides a Nocardia seriola strain and its application. The Nocardia seriola strain is Nocardia seriola B20200724, with a deposit number of CCTCC M 20231950. A nano-immersion vaccine prepared using this strain can prevent largemouth bass nodular disease caused by Nocardia seriola. The vaccine itself is non-toxic and has a relative immune protection rate of 73.3%. It can be used to prevent nocardiosis in largemouth bass during aquaculture, reducing the incidence of the disease and minimizing economic losses during production.

The invention relates to the technical field of environmental monitoring, in particular to a method for measuring soil or water salinity by using microorganisms and application. In a salt environment, the growth rate of the selected microorganisms is related to the salinity; when the salinity is enhanced, the growth of microorganisms is inhibited; according to the method, the salinity of the environment is judged by monitoring microorganisms OD600 in real time; the method comprises the following steps: selecting Nocardia listeria BFY2-1 as a strain to be tested as a microorganism, and selecting Nocardia listeria BFY2-1 as a strain to be tested; a to-be-tested water body or soil 1: 5 suspension; adjusting the pH value to 6-9, and taking 20 ML; adding 10 ML of a three-time culture medium, inoculating a test strain, and comparing with sterile water; carrying out shake cultivation at 28 DEG C, wherein the rotating speed is 200 rpm; after 48 hours, measuring the absorbance at the wavelength of 600 nm by using a spectrophotometer; calculating the salt content according to the absorbance, and converting the initial salt content according to the dilution.

The present invention discloses a strain of Nocardia YINM00009 and its application in the preparation of cyclic lipopeptide compounds, belonging to the field of microbial technology. The Nocardia YINM00009 is an endophyte of Polygonatum daturae in Kunming, Yunnan Province, and can metabolize a large number of novel cyclic lipopeptide compounds after fermentation; the fermentation step mainly includes: fermenting the strain under suitable culture conditions to obtain a fermentation broth, after solvent extraction and concentration, combined with column chromatography, 9 cyclic lipopeptide compounds can be prepared, the total concentration of the 9 cyclic lipopeptide compounds in the fermentation broth can reach 10.2~16.5mg / L, and the purity after extraction and separation can reach more than 99%, of which 2 cyclic lipopeptide compounds have strong anti-tumor cytotoxic activity. The method is low in cost, simple in process, and environmentally friendly, and can be used for the mass production and preparation of such cyclic lipopeptide compounds, which not only meets the needs of a low-carbon economy, but also lays the foundation for the production of cyclic lipopeptide compounds and the development of related products in the later stage.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to nocardia seriolea seriolea resistance traits of lateolabrax japonicus, a method for screening nocardia seriolea seriolea resistance lateolabrax japonicus and application, and particularly belongs to the technical field of aquatic organisms. The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to nocardia seriola seriola resistance traits of lateolabrax japonicus, the nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.1, the 760th basic group of the sequence as shown in SEQ ID NO.1 is an SNP site, and the basic group is A or G; when the SNP molecular marker is AA genotype, the lateolabrax japonicus is judged as the lateolabrax japonicus resistant to nocardia seriolae. The SNP molecular marker is an effective SNP marker for early molecular screening of nocardia seriolae seriolae infection resistance traits of lateolabrax japonicus, disease resistance breeding of lateolabrax japonicus can be achieved, and nocardia seriolae seriolae resistance lateolabrax japonicus is obtained.

The invention belongs to the technical field of prevention and control of diseases of aquatic animals, and discloses application of a biscoumarin derivative in preparation of a medicine for preventing and treating nocardia seriolae diseases of the aquatic animals. The bishydroxycoumarin derivative is 3, 3 '-(ethane-1, 1-diyl) bis (4-hydroxycoumarin), and can be used as an active ingredient for preparing a medicine for preventing and treating bacterial diseases of aquatic animals, and preferably, a mixed feeding use form is adopted. Experiments prove that the minimum in-vitro inhibitory concentration of the derivative to nocardia seriolae is about 3.125 mu g / mL, the survival rate of the derivative in 28 days is 50-56% when the derivative is mixed with largemouth bass infected with the nocardia seriolae at the concentrations of 200 mg / kg and 2000 mg / kg, and the derivative has no obvious toxicity to the largemouth bass within the feed concentration range of 100-3200 mg / kg, and can effectively solve the problems of poor curative effect, residue, drug resistance and the like of traditional antibiotics.

The invention discloses application of curcumin and quercetin combined medicine in preparation of a medicine for treating fish nocardia disease, and belongs to the technical field of aquaculture. The invention develops a new application of curcumin and quercetin combined medicine in the field of aquaculture, and has a wide potential application prospect. On one hand, curcumin and quercetin can be directly used as drugs for preventing and treating nocardia; the curcumin and quercetin combined drug can also be used as a feed additive to prevent and treat nocardiasis infection of fishes. The curcumin and quercetin combined drug provided by the invention has the characteristics of high efficiency, safety, no residue, easiness in degradation, low drug resistance and the like, and provides a new treatment method and way for fish nocardia infection.

The invention relates to a carboxylic acid reductase (MACAR) from Mycobacterium agri, and the carboxylic acid reductase (MACAR) shows higher substrate conversion efficiency than that of a source NiCAR (NiCAR) from Nocardia iowensis, and is suitable for the conversion production of vanillin from vanillic acid. A systematic process for efficient screening and verification of carboxylate reductase CAR identifies a plurality of novel CARs with potential industrial application values, and lays a foundation for directed evolution and industrial application.

The application discloses an egg yolk antibody against Nocardiosis and a preparation method and application thereof, and relates to the technical field of biological medicines. A light chain sequence of the egg yolk antibody comprises a sequence shown in SEQ ID NO. 1, and a heavy chain sequence comprises a sequence shown in SEQ ID NO. 2. The application expands culture of pathogenic bacteria Nocardia, prepares an inactivated bacterin vaccine, and immunizes hens, so that the hens produce egg yolk antibodies against Nocardia through an immune response. After immunization, the application collects eggs, extracts the egg yolk antibodies against Nocardiosis from egg yolk by using a water dilution method. It is verified that the egg yolk antibodies have a strong binding capacity with Nocardia, can effectively inhibit Nocardia, and the titer can reach 1:64000 at the highest.

This invention relates to the X178 strain, which inhibits fungal diseases in grains and removes mycotoxins, and its applications. The X178 strain belongs to the Nocardiaceae family (…). Nocardiopsis This fungus (sp.) is deposited at the China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC No. 34027. Specifically, it exhibits inhibitory effects against grain pathogenic fungi Aspergillus flavus, Aspergillus niger, and Fusarium graminearum, and can remove two mycotoxins: aflatoxin B1 (AFB1) and / or zearalenone.

A use of allicin E for prevention and treatment of Nocardia seriolae infection, comprising: adding a compound as represented by formula (I) to a feed for largemouth bass. The feed can ameliorate the condition of largemouth bass infected with Nocardia, reduce the mortality rate of largemouth bass, and reduce the threat to food and environmental safety caused by long-term use of antibiotics.

The invention belongs to the field of bioengineering pharmacy, and particularly relates to a method for preparing high-standard exemestane through microbial conversion. According to the method, Nocardia simplex Noc (PN)-TS001 is adopted as a fermentation strain, methylenide is efficiently converted by microorganisms to prepare high-standard exemestane, and through optimization and combination of processes of all stages such as methylenide pretreatment, microorganism fermentation conversion and extraction refining, the substrate feeding concentration is increased to 1%-8%, the conversion rate reaches 88%-95%, the total yield reaches 88%-90%, and the yield of exemestane is increased to 90%. The detection content of the prepared exemestane refined product is more than 99.5%, the impurity content is less than 0.1%, and each index meets the USP standard. The method is simple and feasible in process, environment-friendly, low in cost, high in conversion efficiency, high in yield and high in product quality, and has a very good industrial application prospect.

The present invention relates to the field of natural medicine and pharmaceutical technology, and discloses a new compound, novofuran, prepared from Nocardia spp., as well as its preparation method and application. The present invention isolated Nocardia spp. ZHD001 for the first time from sediments collected from the sea area surrounding Zhoushan, and further isolated and studied the fermentation product of Nocardia spp. ZHD001 to obtain novofuran. The present invention determined the chemical structure of the new compound novofuran by analyzing high-resolution mass spectrometry (HRESIMS), hydrogen spectrum, carbon spectrum, HMQC spectrum, HMBC spectrum, and COSY spectrum data of novofuran. A series of studies have shown that novofuran can significantly reduce lipid accumulation in a HepG2 cell lipid model, exhibits lipid-lowering biological activity, and can be used to prepare lipid-lowering drugs or lipid-lowering health foods, showing excellent development and application prospects.

The invention relates to an aquatic feed additive for resisting nocardia infection and a preparation method thereof, and belongs to the technical field of aquaculture, the aquatic feed additive comprises 2-8% by weight of polypeptide, and the polypeptide has an amino acid sequence as shown in SEQ ID NO: 1. According to the application, the efficient, safe and sustainable nocardia-resistant feed additive is successfully developed by using the polypeptide derived from fish blood protein enzymolysis, an ideal alternative scheme is provided for solving intractable bacterial infection in aquaculture, and the nocardia-resistant feed additive has remarkable economic benefits and ecological benefits.

The invention discloses a nocardia seriolae mycotic acid extraction and foam cell model induction method, and belongs to the crossing field of aquatic disease control and pathogenic biology, and the nocardia seriolae mycotic acid extraction and foam cell model induction method comprises the following steps: S1, mycotic acid extraction, and S2, foam cell model induction. A reliable material basis is provided for follow-up research from thallus culture to high-purity extract obtaining, when an induced foam cell model is constructed, detailed and normative operation steps cover macrophage separation, microsphere coating and co-culture detection, the in-vivo infection process can be accurately simulated, all links are closely connected, the steps are clear, repeatability is high, and the method is suitable for large-scale popularization and application. The method has the advantages that the method is convenient for other researchers to use for reference and verification, multi-dimensional research design can comprehensively explore the action mechanism of mycolic acid from cell and living body levels, deep understanding of nocardia seriolae nocardia pathogenesis is facilitated, and powerful theoretical basis and technical support are provided for prevention and control of fish nocardia diseases.

The invention relates to a kit for detecting nocardia seriolae of jewfish based on RPA-CRISPR / Cas12a as well as a detection method and application of the kit. The main component of the kit is a composition for identifying nocardia seriolae, and the composition comprises a specific primer for detecting nocardia seriolae based on RPA-CRISPR / Cas12a, a Cas12a protein, crRNA and a fluorescence reporter probe ssDNA Reporter, wherein the specific primer, the Cas12a protein, the crRNA and the fluorescence reporter probe are used for detecting nocardia seriolae based on RPA-CRISPR / Cas12a. The detection method of the kit comprises the following steps: S1, extracting a sample genome DNA; s2, carrying out RPA (recombinase polymerase amplification); s3, CRISPR (clustered regularly interspaced short palindromic repeats) / And S4, interpreting a detection result. According to the invention, a target gene segment of nocardia seriolae is rapidly amplified at a constant temperature by virtue of an RPA technology, and the target gene segment of nocardia seriolae is specifically targeted by virtue of accurate recognition and trans-cleavage capabilities of CRISPR / Cas12a, so that rapid detection visible to naked eyes is realized. Compared with conventional detection methods such as PCR, qPCR and the like, the kit does not need a professional thermal cycler, has the advantages of high sensitivity, strong specificity, simplicity and convenience in operation, low cost and the like, and is expected to be applied to on-site instant detection of aquaculture sites and basic laboratories.

The application discloses a real-time fluorescent PCR detection primer pair and probe combination of Nocardioides nucleic acid, a kit, a method and application, a nucleotide sequence of an upstream primer F of the primer pair is shown as SEQ ID:NO.1, and a nucleotide sequence of a downstream primer R is shown as SEQ ID:NO.2; the probe is a TaqMan probe with a fluorescent group FAM at a 5' end and a fluorescent quenching group BHQ1 at a 3' end, and a nucleotide sequence of the probe is shown as SEQ ID:NO.3. 0 The application can stably detect the Nocardioides at a low detection concentration of 5.62x10 0 copy / μL, can quickly, accurately and quantitatively detect the Nocardioides, has good specificity and can detect different sub-species of the Nocardioides, and has good universality.

The application provides a vaccine for Nocardia seriolae and a preparation method and application thereof. The vaccine for Nocardia seriolae takes an ATP binding protein (ABP) of an ABC family on a membrane protein of Nocardia seriolae as a candidate antigen, a primer is designed according to a gene sequence of the ABP, and the ABP is cloned into a vector to obtain a recombinant plasmid and is prepared. An amino acid sequence of the ABP is shown as SEQ ID No: 6, and a nucleotide sequence of a coding gene of the ABP is shown as SEQ ID No: 5. An engineering strain containing a prokaryotic expression recombinant plasmid of the ABP is cultured, fermentation, crushing and purification are performed to obtain a recombinant protein vaccine; an engineering strain containing a eukaryotic expression recombinant plasmid of the ABP is cultured, and a large amount of endotoxin-free recombinant plasmid is extracted to obtain a DNA vaccine; and a eukaryotic recombinant plasmid wrapped by mannose chitosan is used as a targeted DNA vaccine. The recombinant protein vaccine, the DNA vaccine and the targeted DNA vaccine prepared by the application have good prevention and treatment effects on Nocardia seriolae disease.

The invention relates to the technical field of microorganisms, in particular to a nocardia seriolae bacteriophage with high thermal stability as well as a composition and application of the nocardia seriolae bacteriophage. The name of the nocardia seriolae bacteriophage is RDP-NS004, the nocardia seriolae bacteriophage is preserved in the China General Microbiological Culture Collection Center on August 25, 2025, and the preservation number of the nocardia seriolae bacteriophage is CGMCC No. 46675. The nocardia seriolae bacteriophage is named as RDP-NS004, and the nocardia seriolae bacteriophage is preserved in the China General Microbiological Culture Collection Center on August 25, 2025. The composition comprises the bacteriophage. The bacteriophage or the composition is prepared in the following steps: (1) preparing a product for killing nocardia seriolae; (2) preparing a product for inhibiting nocardia seriola; and (3) application in preparation of a product for preventing and / or treating fish nocardia disease caused by nocardia seriolae. When the bacteriophage acts for 60 minutes at 50 DEG C, the titer can still be maintained at 106 PFU / mL, and the bacteriophage has the characteristic of high thermal stability. The separation of the nocardia seriola bacteriophage with high heat stability can effectively solve the problem that the conventional bacteriophage is limited in effect in a high-temperature environment, and can better prevent and control bacterial diseases in the high-incidence period of nocardia seriola in summer.

The invention discloses nocardia globulus DSM 44596 and an application of the nocardia globulus DSM 44596 in tung tree fruit compost. The nocardia globerula DSM 44596 is preserved in the Guangdong Microbial Culture Collection Center on September 29, 2025, the classification name of the nocardia globerula is nocardia globerula, the preservation number of the nocardia globerula is GDMCC No: 67055, and the preservation address of the nocardia globerula DSM 44596 is the fifth floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou. The Nocardia globulus DSM 44596 can effectively solve the key problems that tung tree fruit compost is slow in maturing, low in nutrient effectiveness and the like through the dual effects of synchronously enhancing organic matter degradation and nutrient activation. The invention provides an efficient microbial technical approach for resource utilization of high-grease and high-lignocellulose agricultural wastes.

The application provides a SNP molecular marker related to a Nocardia seriolae resistance trait of Epinephelus akaara, a method for screening the Nocardia seriolae resistance Epinephelus akaara and application, and specifically belongs to the field of aquatic organism technology. The application provides a SNP molecular marker related to a Nocardia seriolae resistance trait of Epinephelus akaara, wherein the nucleotide sequence of the SNP molecular marker is shown in SEQ ID NO. 1, the base at the 760th position of the sequence shown in SEQ ID NO. 1 is a SNP site, and the base is A or G; when the SNP molecular marker is an AA genotype, it is determined that the Epinephelus akaara is resistant to Nocardia seriolae. The SNP molecular marker provided by the application is an effective SNP marker for early molecular screening of the Nocardia seriolae resistance trait of Epinephelus akaara, can realize disease resistance breeding of Epinephelus akaara, and obtain Epinephelus akaara resistant to Nocardia seriolae.

The invention relates to the technical field of aquaculture and disease prevention and control, and discloses a green prevention and control method for fish nocardia disease, which comprises the following steps: preparing a multifunctional targeted nano delivery system, and mixing the multifunctional targeted nano delivery system with fish feed to prepare functional feed for feeding. The multifunctional targeting nano delivery system takes a pH sensitive polymer skeleton as a carrier, the surface of the pH sensitive polymer skeleton is connected with a mannose ligand capable of targeting macrophages, and a double-effect antibacterial core composed of an antibacterial agent and a quorum sensing inhibitor is encapsulated in the pH sensitive polymer skeleton. After entering a fish body through a feed, the system can actively target and enter macrophages in a lesion area, and responsively disintegrate and release drugs in an intracellular acidic microenvironment. According to the invention, a targeted delivery strategy is utilized, active ingredients are successfully delivered to intracellular focuses which traditional drugs are difficult to reach, the problem of treatment of nocardia granuloma is solved, and the preparation has the advantages of strong targeting, synergistic interaction, greenness and safety, and provides a new way for prevention and control of intractable bacterial diseases of fishes.

The invention discloses a group of nocardia seriolae trehalose synthase genes and a construction method of a deletion strain of the nocardia seriolae trehalose synthase genes, and belongs to the technical field of nocardia seriolae research. Comprising the following steps: nocardia seriolae culture and whole genome extraction, trehalose synthase gene cloning, deletion plasmid construction, competent cell preparation, electrotransformation and deletion strain screening, and deletion strain feature verification. Trehalose synthase genes of nocardia seriolae tpp, treS, treZ and the like are cloned, and the deletion strain is successfully constructed by using a homologous recombination technology, so that the key effect of the genes in the trehalose synthesis of the nocardia seriolae is defined, the close association of the trehalose content with the strain form, growth characteristics and toxicity is disclosed, and the application prospect is wide. The method provides important theoretical support for deep analysis of nocardia seriolae nocardia pathogenesis, and lays a solid foundation for novel prevention and control target screening and prevention and control strategy formulation of fish nocardia seriolae.

A corn field long residual herbicide degrading bacterium HK-1 is classified as Nocardia (Nocardia sp.) Nocardia sp. ), which was preserved in China General Microbiological Culture Collection Center on January 31, 2023, with a preservation number of CGMCC No. 26497 and a preservation address of No. 1, Xibaixili, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences. The preparation method is simple, the production cost is low, the high-efficiency degrading bacterium HK-1 can efficiently degrade the long residual herbicides of benzofluor and atrazine under the conditions of acid, alkaline environment, lack of nitrogen source and high-concentration herbicide pollution, the degradation effect on the soil polluted by the combined benzofluor and atrazine herbicides in corn field is remarkable, the bacterium can survive in the soil for a long time in the form of saprophyte, can promote crop growth and reduce the probability of crop disease, and has remarkable economic and social benefits.

The invention relates to an X178 strain for inhibiting fungal diseases of grains and removing mycotoxin and application of the X178 strain. The X178 strain belongs to Nocardia sp. And is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the X178 strain is CGMCC No. 34027. Specifically, the compound has an inhibition effect on grain pathogenic fungi such as aspergillus flavus, aspergillus niger and fusarium graminearum, and can remove two mycotoxins, namely aflatoxin B1 (AFB1) and / or zearalenone.

The invention discloses a genetic engineering strain for efficiently expressing nitrile hydratase as well as a construction method and application of the genetic engineering strain. On the basis of a nitrile hydratase (PtNHase) gene of pseudonocardia thermophila, the following optimization and transformation are carried out: (1) carrying out complete sequence optimization on a gene sequence according to codon preference of escherichia coli; (2) replacing a ribosome binding site (SD sequence) at the upstream of the alpha subunit; and (3) carrying out site-specific mutagenesis on tryptophan (Trp) at the 72nd site of the beta subunit to obtain phenylalanine (Phe). The nitrile hydratase produced by the engineering strain constructed by the invention has significantly improved enzyme activity, thermal stability and tolerance to a substrate / product, can be used for industrial biosynthesis of acrylamide and nicotinamide and degradation of nitrile-containing pollutants, is suitable for the fields of industrial large-scale production and environmental governance, and has broad application prospects.

The invention relates to the technical field of biology, in particular to a method for improving the protein expression quantity, a recombinant plasmid and a construction method and application of the recombinant plasmid, a natural Retron element of pseudonocardia maple shown as SEQ ID NO.6 is integrated in a pE15A carrier skeleton, a target protein expression cassette driven by a weak promoter is inserted into an MSD area of the Retron element Pa135, and an expression plasmid pRetron-X is obtained; and transforming the pRetron-X plasmid into a host competent cell so as to express the target protein. The method provided by the invention efficiently improves the expression quantity of the target protein.

The present application relates to the field of microbial technology, in particular to a Nocardia seriolae bacteriophage with high thermal stability and a composition and application thereof. The Nocardia seriolae bacteriophage is named RDP-NS004, which was preserved in the China General Microbiological Culture Collection Center on August 25, 2025, and the preservation number is CGMCC No. 46675. A composition comprises the above-mentioned bacteriophage. The above-mentioned bacteriophage or composition is used in (1) preparation of a product for killing Nocardia seriolae; (2) preparation of a product for inhibiting Nocardia seriolae; (3) preparation of a product for preventing and / or treating fish Nocardiosis caused by Nocardia seriolae. The bacteriophage of the present application can maintain a titer of 10 6 PFU / mL at 50 DEG C for 60 min, and has the characteristics of high thermal stability. The isolation of the Nocardia seriolae bacteriophage with high thermal stability can effectively solve the problem that the conventional bacteriophage is limited in effect in a high-temperature environment, and better prevent and control the bacterial disease problem faced in the high-incidence period of Nocardiosis in summer.