43 results about "Nocardia sp" patented technology

The invention provides a microbial composition comprising acetobacter aceti, beauveria bassiana, azospirllum brasilense, nocardia cellulans, bacillus mucilaginosus, metarhizium anisopliae, trichoderma aureoviride and the like. The microbial composition can be stably combined in a microbial agent, is used for improving soil / land and especially can be used for effectively improving soil polluted by heavy metals.

The invention provides a microbial composition comprising acetobacter aceti, beauveria bassiana, azospirllum brasilense, nocardia cellulans, bacillus mucilaginosus, metarhizium anisopliae, trichoderma aureoviride and the like. The microbial composition can be stably combined in a microbial agent, can survive for a long term in a plurality of improved soil to play a role, is good in adaptation of improving soil / land and especially can be used for effectively improving a plurality of soil, such as secondary salinization land, desertification soil.

The invention belongs to the fields of biotechnology and genetic engineering, in particular relates to a high-efficiency streptomyces minitype transposable mutation system as well as a construction method and application thereof. Based on a transposable element sourcing from Norcardia aseteroids YP21, the invention constructs the characteristics of action sites of IS204 transposase in streptomyces coelicolor, and the transposable system is proved to have good genetic stability and good transposable randomness. Transposable mutation is carried out on S. coelicolor M145 by utilizing pDZY101 and derivatives thereof to obtain a great quantity of phenotype mutant strains, in addition, similar characteristics are also found in streptomyces lividans and other streptomyces members, and the characteristics endow the transposable system with good application prospect in aspect of researching type streptomyces functional genes.

The invention provides modification and application of nitrile hydratase amino acid motif, and belongs to the technical field of bioengineering. According to the invention, nitrile hydratase derived from pseudonocardia thermophila is modified, the NO.46 site on a beta subunit and the NO.6 site and NO.126 site on an alpha subunit of a wild type are mutated respectively, and the specific enzyme activity of a constructed mutant is remarkably improved and can be improved by 1-5 times compared with that of the wild type. Moreover, the substrate spectrum is also broadened, the mutant has a very goodcatalytic effect on isobutyronitrile, n-valeronitrile, acrylonitrile, nicotinonitrile, 2-cyanopyrazine, benzonitrile, cinnamonitrile, naphthonitrile and the like, and the catalytic performance on corresponding substrates is also remarkably improved. Therefore, the obtained nitrile hydratase mutant can be suitable for more production scenes, and the production efficiency is improved.

The invention provides a sulfonamide antibiotic synergistically degraded bacteria and an application thereof. The sulfonamide antibiotic synergistically degraded bacteria comprise arthrobacterium P27and nocardioides N27, wherein the arthrobacterium P27 is preserved in China General Microbiological Culture Collection Center on December 03, 2019, and the preservation number is CGMCC No. 19070; andthe nocardioides N27 is preserved in the China General Microbiological Culture Collection Center on December 03, 2019, and the preservation is CGMCC NO. 19071. According to the technical scheme, compounding of the two strains of bacteria can synergistically complete degradation of sulfamethoxazole and an azacyclo product 3-amino-5-methylisoxazole; and in addition, the strain P27 can degrade one side of benzene rings of various sulfonamide antibiotics, the degradation substrate spectrum is wide, and a new idea and a new strain resource are provided for deep removal of the sulfonamide antibiotics.

The invention discloses a new Chemomycin A and preparing method and drug component and application in the tumour prevention drug, which is characterized by the following: separating Angucycline from Nocardia Mediterranei Var. Kanglensis 1747-64 to obtain the product; displaying excellent anti-tumour activity in the external activity test.

The invention discloses a Nocardia sp. WY1202 and a production method for (R)-3-quinuclidinol through fermentation of quininone by using Nocardia sp. WY1202. According to the invention, quininone hydrochloride is used as a sole carbon source for the strain of Nocardia sp. WY1202 which is screened from soil in a farm orchard in Xiqing District, Tianjin City, China, and is preserved in China General Microbiological Collection Center with an accession number of CGMCC No. 5095. The production method comprises the following steps: with the Nocardia sp.WY1202 as a strain, carrying out primary seed culture and secondary fermentation amplification culture; taking a fermentation culture solution, collecting thalli through centrifugation, washing the thalli and re-suspending the thalli in a buffer solution; adding the substrate quininone hydrochloride, adding glucose and carrying out centrifugation after a reaction for 24 to 48 h; adjusting the pH value of a supernatant until the supernatant is alkaline; drying the supernatant through pressure reduced spinning; subjecting an obtained residue to solid-liquid extraction with an organic solvent; and drying an obtained filtrate through spinning so as to obtain (R)-(-)-3-quinuclidinol. According to the invention, the product (R)-(-)-3-quinuclidinol has a yield of 95%, a purity of 96% and an e.e value of more than 95%; the production method is simple to operate, has low energy consumption, accords with requirements for green chemistry and is applicable to large-scale bioconversion production for (R)-(-)-3-quinuclidinol.

Disclosed are a high specific active protease congenetic with the protease from imitative Nocard's bacillus, and the production in the wild type and the reconstructed host-cells containing the transgenic plant and the no-human transgenic animal cells. The protease is effective in animal feed and washing agent. Peculiar structural characteristics related to the high specific activity of protease are also disclosed. The protease belongs to the peptidase family S2A or S1E.

The present invention relates to a group of novel compound Chemomicin B,C,D and its preparation method, pharmaceutical composition containing Chemomicin B,C,D as active ingredients and its uses of Chemomicin B,C,D in preparing antineoplastic drug. Chemomicin B,C,D is Angucyclinone antibiotic separated from Nocardia Mediterranei Var.Kanglensis 1747-64 culture. In vitro activity test prove Chemomicin B,C,D has strong suppressing activity for human oesophagus cancer cell KYSE150 and has good development prospect as a novel antineoplastic drug.

The invention belongs to the technical field of the biological medicine, and relates to a microbial conversion method for a euphorbia lathyris diterpene compound. The method comprises the following steps: performing the microbial conversion to euphorbia lathyris phorbol and 7-hydroxy euphorbia lathyris phorbol by using Nocard's bacillus (i) Nocardia sp. ( / i) NRRL 5646, and preparing 3-carbonyl euphorbia lathyris phorbol and 7-acetoxyl euphorbia lathyris phorbol derivatives. Proved by the experiment, the polarity of the 3-carbonyl euphorbia lathyris phorbol in the derivative is reduced. Compared with a substrate, the derivative has the better cytotoxic activity. The derivative is an antitumor lead compound with development value.

The invention provides a preparation method of Nocardia competent cells and a gene editing method of Nocardia. According to the preparation method, Nocardia is treated with lysozyme so that the thickness of cellular wall of Nocardia is deceased; Nocardia competent cells easily accepting exogenous DNAs are finally prepared. The preparation method is simple and quick to perform and has high application value. A CRSIPR / Cas9 system is applied to Nocardia for the first time herein according to the gene editing method of Nocardia; a CRISPR / Cas9 genetic editing plasmid is constructed and is convertedand delivered to Nocardia for editing a target gene. The gene editing method is simple and quick to perform, has high application value and helps lay the basis for the study on gene functionality ofNocardia, the construction of gene deleted strains of Nocardia and the preparation of gene deleted vaccines of Nocardia.

The invention discloses an alkali-resisting nocardiopsis for producing ansamycin P-3 and 15-hydroxyl derivatives as well as a preparation method and the application of the nocardiopsis. The provided strain is nocardiopsis EGI 80425, and the collection number of the nocardiopsis EGI 80425 is CGMCC No. 9969; a good technical effect can be obtained in the application of the strain in preparing the ansamycin P-3 and the 15-hydroxyl derivatives thereof. Tests prove that a remarkable and obvious technical effect can be obtained in the application of the strain nocardiopsis EGI 80425 CGMCC No. 9969 in producing the ansamycin P-3 and the 15-hydroxyl derivatives thereof, and therefore, the strain has excellent application prospect in producing the ansamycin P-3 and the 15-hydroxyl derivatives thereof.

The invention is applicable to the technical field of genetic engineering and provides a nocardia seriolae attenuated strain and a preparation method and application thereof. The nocardia seriolae attenuated strain is a nocardia seriolae strain containing alanine dehydrogenase gene deletion. The preparation method comprises the following steps of: connecting an upstream fragment and a downstream fragment of an alanine dehydrogenase gene into a vector to obtain a recombinant vector; taking a nocardia seriolae wild strain to prepare nocardia seriolae competent cells; performing electrotransformation on the nocardia seriolae competent cells by utilizing the recombinant vector to obtain electrotransformation bacterial liquid; and culturing and screening the electrotransformation bacterial liquid to obtain the nocardia seriolae attenuated strain. The nocardia seriolae attenuated strain provided by the invention can be used for preventing nocardiosis infection; after the alanine dehydrogenase gene is deleted by a gene knockout method, the pathogenicity of bacteria can be effectively reduced, but relatively good immunogenicity is still retained.

The invention belongs to the field of oily sewage treatment, and particularly relates to a bacterial strain for producing a biological flocculant and the biological flocculant produced by using the bacterial strain. The bacterial strain of the biological flocculant produced by the invention is named as Nocardia rhodochroms, and the preservation number is CGMCC NO.19718. The strain is preserved inChina General Microbiological Culture Collection Center on April 24 in 2020. The strain is stable in performance, and the biological flocculant for treating oily sewage can be prepared in the fermentation process. Therefore, the oily sewage can be well treated, and the treatment effect is superior to that of the existing common Nocardia rhodochroms. Moreover, the biological flocculant prepared from the strain does not cause harm to the environment, and the technical problems that an existing treatment method easily causes secondary pollution and has residual toxicity are solved.

The present invention relates to biological flocculant, and is especially biological flocculant XM-11 fermenting process with controlled staged oxygen supply. Microbe Nocardia sp. XM-B11 is cultured in the fermenting culture medium through the seed culturing in the inoculation amount of 8-15 % for 8-16 hr and the liquid fermentation at 25-30 deg.c for 30-56 hr including the initial fermentation stage of 0-20 hr in stirring rotation speed of 100-150 rpm. The process can ensure the excellent growth of the spawn in the initial fermentation stage and the optimized composing state of the biological flocculant in the later fermentation stage, and has low power consumption and low production cost.

The invention relates to application of a Nocardia rubra cell wall skeleton as a neutrophil modulator. The present application provides a neutrophil modulator, which comprises a component derived from the cell wall of Nocardia rubra, and which can modulate the chemotactic function, phagocytosis function, and bactericidal function (reactive oxygen species (ROS) generation, degranulation formation) of neutrophil. The neutrophil regulator disclosed by the invention can also improve the local immune microenvironment of a chronic infection wound.

The invention relates to autotrophic pseudonocardia and application thereof, and belongs to the technical field of microorganisms. The pseudonocardia autotrophicus can be used for preparing calcifediol and / or calcitriol, compared with an original strain, the yield is remarkably improved.

The invention belongs to the technical field of microorganisms, and particularly relates to an application of a nocardia immunopotentiator in preparation of poultry vaccines. An immunologic adjuvant is prepared by culturing nocardia through a fermentation tank, sterilizing at high pressure and extracting. The nocardia culture or fermentation product is used as the immunologic adjuvant for preparing the poultry vaccine, so that the nocardia vaccine has the advantages of high safety, no residue in vaccine injection and capability of remarkably improving the immunity of animals.

The invention discloses a strain of Nocardia anfenacea bacteriophage P69 and application thereof, and belongs to the technical field of biology. The preservation number of the nocardia orientalis bacteriophage P69 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.23088. The nocardia orientalis bacteriophage P69 provides a selectable basic material for prevention and control of a sludge foaming event caused by nocardia orientalis, and enriches a bacteriophage seed bank; the functional gene and protein of the bacteriophage P69 are further deeply excavated through separation identification and whole genome sequencing of the bacteriophage P69, and one or more enzymes and the like with a splitting effect on Nocardia soufeniana are provided for bacteriophage treatment and biological prevention and control, so that the host spectrum is expanded, and the application universality and effectiveness of the bacteriophage are improved; the bacteriophage P69 can be used for rapidly and efficiently cracking Nocardia soufeniformis and can be used for sludge foaming events caused by excessive propagation of Nocardia in a sewage treatment system.

The invention relates to a PCR detection method of pathogenic bacteria of fish sarcoidosis, comprising the following steps of: extracting genomic DNA of Aeromonas hydrophila, Photobacterium damselae subsp. piscicida, Nocardia asteroids, Aeromonas schubertii, Nocardia seriolae and Nocardia salmonicida; carrying out primer design on the genomic DNA of pathogenic bacteria; and carrying out amplification and detection on the genomic DNA of Aeromonas hydrophila, Photobacterium damselae subsp. piscicida, Nocardia asteroids, Aeromonas schubertii, Nocardia seriolae and Nocardia salmonicida. Various pathogenic bacteria of sarcoidosis can be detected by the method, and the method has high specificity and sensitivity.

The invention discloses a rhodococcus circulans phage P19 and application thereof, and belongs to the technical field of biology. The preservation number of the nocardia pekinensis bacteriophage P19 disclosed by the invention is CGMCC No.23087. The nocardia pekinensis bacteriophage P19 provides a selectable basic material for prevention and control of a sludge foaming event caused by rhodococcus pluvialis, and enriches a bacteriophage seed bank; through separation identification and whole genome sequencing of the bacteriophage P19, functional genes and proteins of the bacteriophage P19 are further deeply excavated, and one or more enzymes and the like with a splitting effect on rhodococcus circulans are provided for bacteriophage treatment and biological prevention and treatment, so that a host spectrum is expanded, and the application universality and effectiveness of the bacteriophage are improved; the bacteriophage P19 can rapidly and efficiently split rhodococcus circulans, and can be used for sludge foaming events caused by excessive propagation of rhodococcus circulans in a sewage treatment system.

The invention is applicable to the field of fish pathogenic microbiology, and provides a chromogenic solid culture medium of nocardia seriolae as well as preparation method and application. The chromogenic solid culture medium comprises the following components: brain heart leachate broth culture medium powder, agar powder, esculin, ferric citrate, an antibiotic composition, distilled water and culture supernatant of nocardia seriolae. The culture medium with the characteristics of short separation time, high sensitivity, high isolated bacterium purity, simple preparation method and the like can be prepared by adding the esculin, the ferric citrate, the culture supernatant of the nocardia seriolae in the logarithmic phase and the antibiotic composition on the basis of the basic formula ofthe brain heart leachate broth agar culture medium. The culture medium has the characteristics of high separation efficiency, high separation purity, convenience in observation and the like when beingused for separating nocardia seriola, and can be effectively applied to the separation of nocardia seriola in clinical sample.

The invention discloses nocardioides. The nocardioides is classified and named as nocardioides ST46 in microbiology, and is named as nocardioides sp. in Latin scientific name. The 16S rDNA partial sequence is as shown in a sequence table; and the preservation number of the strain is CGMCC 4.7686. The invention further discloses a culture and separation method of the nocardioides and application of the nocardioides in preparation of tobacco flavor. The nocardioides is a new strain, the tobacco flavor prepared from the nocardioides is used for flavoring cigarettes, the richness of tobacco flavor and the fullness of smoke are remarkably improved, the smoke strength is remarkably improved, and meanwhile, the aftertaste and comfort are good.