13 results about "Nocardia sp" patented technology

The invention relates to the technical field of environmental monitoring, in particular to a method for measuring soil or water salinity by using microorganisms and application. In a salt environment, the growth rate of the selected microorganisms is related to the salinity; when the salinity is enhanced, the growth of microorganisms is inhibited; according to the method, the salinity of the environment is judged by monitoring microorganisms OD600 in real time; the method comprises the following steps: selecting Nocardia listeria BFY2-1 as a strain to be tested as a microorganism, and selecting Nocardia listeria BFY2-1 as a strain to be tested; a to-be-tested water body or soil 1: 5 suspension; adjusting the pH value to 6-9, and taking 20 ML; adding 10 ML of a three-time culture medium, inoculating a test strain, and comparing with sterile water; carrying out shake cultivation at 28 DEG C, wherein the rotating speed is 200 rpm; after 48 hours, measuring the absorbance at the wavelength of 600 nm by using a spectrophotometer; calculating the salt content according to the absorbance, and converting the initial salt content according to the dilution.

The application discloses an egg yolk antibody against Nocardiosis and a preparation method and application thereof, and relates to the technical field of biological medicines. A light chain sequence of the egg yolk antibody comprises a sequence shown in SEQ ID NO. 1, and a heavy chain sequence comprises a sequence shown in SEQ ID NO. 2. The application expands culture of pathogenic bacteria Nocardia, prepares an inactivated bacterin vaccine, and immunizes hens, so that the hens produce egg yolk antibodies against Nocardia through an immune response. After immunization, the application collects eggs, extracts the egg yolk antibodies against Nocardiosis from egg yolk by using a water dilution method. It is verified that the egg yolk antibodies have a strong binding capacity with Nocardia, can effectively inhibit Nocardia, and the titer can reach 1:64000 at the highest.

This invention relates to the X178 strain, which inhibits fungal diseases in grains and removes mycotoxins, and its applications. The X178 strain belongs to the Nocardiaceae family (…). Nocardiopsis This fungus (sp.) is deposited at the China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC No. 34027. Specifically, it exhibits inhibitory effects against grain pathogenic fungi Aspergillus flavus, Aspergillus niger, and Fusarium graminearum, and can remove two mycotoxins: aflatoxin B1 (AFB1) and / or zearalenone.

A use of allicin E for prevention and treatment of Nocardia seriolae infection, comprising: adding a compound as represented by formula (I) to a feed for largemouth bass. The feed can ameliorate the condition of largemouth bass infected with Nocardia, reduce the mortality rate of largemouth bass, and reduce the threat to food and environmental safety caused by long-term use of antibiotics.

The invention relates to an aquatic feed additive for resisting nocardia infection and a preparation method thereof, and belongs to the technical field of aquaculture, the aquatic feed additive comprises 2-8% by weight of polypeptide, and the polypeptide has an amino acid sequence as shown in SEQ ID NO: 1. According to the application, the efficient, safe and sustainable nocardia-resistant feed additive is successfully developed by using the polypeptide derived from fish blood protein enzymolysis, an ideal alternative scheme is provided for solving intractable bacterial infection in aquaculture, and the nocardia-resistant feed additive has remarkable economic benefits and ecological benefits.

The application discloses a real-time fluorescent PCR detection primer pair and probe combination of Nocardioides nucleic acid, a kit, a method and application, a nucleotide sequence of an upstream primer F of the primer pair is shown as SEQ ID:NO.1, and a nucleotide sequence of a downstream primer R is shown as SEQ ID:NO.2; the probe is a TaqMan probe with a fluorescent group FAM at a 5' end and a fluorescent quenching group BHQ1 at a 3' end, and a nucleotide sequence of the probe is shown as SEQ ID:NO.3. 0 The application can stably detect the Nocardioides at a low detection concentration of 5.62x10 0 copy / μL, can quickly, accurately and quantitatively detect the Nocardioides, has good specificity and can detect different sub-species of the Nocardioides, and has good universality.

The application provides a vaccine for Nocardia seriolae and a preparation method and application thereof. The vaccine for Nocardia seriolae takes an ATP binding protein (ABP) of an ABC family on a membrane protein of Nocardia seriolae as a candidate antigen, a primer is designed according to a gene sequence of the ABP, and the ABP is cloned into a vector to obtain a recombinant plasmid and is prepared. An amino acid sequence of the ABP is shown as SEQ ID No: 6, and a nucleotide sequence of a coding gene of the ABP is shown as SEQ ID No: 5. An engineering strain containing a prokaryotic expression recombinant plasmid of the ABP is cultured, fermentation, crushing and purification are performed to obtain a recombinant protein vaccine; an engineering strain containing a eukaryotic expression recombinant plasmid of the ABP is cultured, and a large amount of endotoxin-free recombinant plasmid is extracted to obtain a DNA vaccine; and a eukaryotic recombinant plasmid wrapped by mannose chitosan is used as a targeted DNA vaccine. The recombinant protein vaccine, the DNA vaccine and the targeted DNA vaccine prepared by the application have good prevention and treatment effects on Nocardia seriolae disease.

The invention discloses nocardia globulus DSM 44596 and an application of the nocardia globulus DSM 44596 in tung tree fruit compost. The nocardia globerula DSM 44596 is preserved in the Guangdong Microbial Culture Collection Center on September 29, 2025, the classification name of the nocardia globerula is nocardia globerula, the preservation number of the nocardia globerula is GDMCC No: 67055, and the preservation address of the nocardia globerula DSM 44596 is the fifth floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou. The Nocardia globulus DSM 44596 can effectively solve the key problems that tung tree fruit compost is slow in maturing, low in nutrient effectiveness and the like through the dual effects of synchronously enhancing organic matter degradation and nutrient activation. The invention provides an efficient microbial technical approach for resource utilization of high-grease and high-lignocellulose agricultural wastes.

The application provides a SNP molecular marker related to a Nocardia seriolae resistance trait of Epinephelus akaara, a method for screening the Nocardia seriolae resistance Epinephelus akaara and application, and specifically belongs to the field of aquatic organism technology. The application provides a SNP molecular marker related to a Nocardia seriolae resistance trait of Epinephelus akaara, wherein the nucleotide sequence of the SNP molecular marker is shown in SEQ ID NO. 1, the base at the 760th position of the sequence shown in SEQ ID NO. 1 is a SNP site, and the base is A or G; when the SNP molecular marker is an AA genotype, it is determined that the Epinephelus akaara is resistant to Nocardia seriolae. The SNP molecular marker provided by the application is an effective SNP marker for early molecular screening of the Nocardia seriolae resistance trait of Epinephelus akaara, can realize disease resistance breeding of Epinephelus akaara, and obtain Epinephelus akaara resistant to Nocardia seriolae.

The invention discloses a group of nocardia seriolae trehalose synthase genes and a construction method of a deletion strain of the nocardia seriolae trehalose synthase genes, and belongs to the technical field of nocardia seriolae research. Comprising the following steps: nocardia seriolae culture and whole genome extraction, trehalose synthase gene cloning, deletion plasmid construction, competent cell preparation, electrotransformation and deletion strain screening, and deletion strain feature verification. Trehalose synthase genes of nocardia seriolae tpp, treS, treZ and the like are cloned, and the deletion strain is successfully constructed by using a homologous recombination technology, so that the key effect of the genes in the trehalose synthesis of the nocardia seriolae is defined, the close association of the trehalose content with the strain form, growth characteristics and toxicity is disclosed, and the application prospect is wide. The method provides important theoretical support for deep analysis of nocardia seriolae nocardia pathogenesis, and lays a solid foundation for novel prevention and control target screening and prevention and control strategy formulation of fish nocardia seriolae.

The invention discloses a genetic engineering strain for efficiently expressing nitrile hydratase as well as a construction method and application of the genetic engineering strain. On the basis of a nitrile hydratase (PtNHase) gene of pseudonocardia thermophila, the following optimization and transformation are carried out: (1) carrying out complete sequence optimization on a gene sequence according to codon preference of escherichia coli; (2) replacing a ribosome binding site (SD sequence) at the upstream of the alpha subunit; and (3) carrying out site-specific mutagenesis on tryptophan (Trp) at the 72nd site of the beta subunit to obtain phenylalanine (Phe). The nitrile hydratase produced by the engineering strain constructed by the invention has significantly improved enzyme activity, thermal stability and tolerance to a substrate / product, can be used for industrial biosynthesis of acrylamide and nicotinamide and degradation of nitrile-containing pollutants, is suitable for the fields of industrial large-scale production and environmental governance, and has broad application prospects.

The invention relates to the technical field of biology, in particular to a method for improving the protein expression quantity, a recombinant plasmid and a construction method and application of the recombinant plasmid, a natural Retron element of pseudonocardia maple shown as SEQ ID NO.6 is integrated in a pE15A carrier skeleton, a target protein expression cassette driven by a weak promoter is inserted into an MSD area of the Retron element Pa135, and an expression plasmid pRetron-X is obtained; and transforming the pRetron-X plasmid into a host competent cell so as to express the target protein. The method provided by the invention efficiently improves the expression quantity of the target protein.

The present application relates to the field of microbial technology, in particular to a Nocardia seriolae bacteriophage with high thermal stability and a composition and application thereof. The Nocardia seriolae bacteriophage is named RDP-NS004, which was preserved in the China General Microbiological Culture Collection Center on August 25, 2025, and the preservation number is CGMCC No. 46675. A composition comprises the above-mentioned bacteriophage. The above-mentioned bacteriophage or composition is used in (1) preparation of a product for killing Nocardia seriolae; (2) preparation of a product for inhibiting Nocardia seriolae; (3) preparation of a product for preventing and / or treating fish Nocardiosis caused by Nocardia seriolae. The bacteriophage of the present application can maintain a titer of 10 6 PFU / mL at 50 DEG C for 60 min, and has the characteristics of high thermal stability. The isolation of the Nocardia seriolae bacteriophage with high thermal stability can effectively solve the problem that the conventional bacteriophage is limited in effect in a high-temperature environment, and better prevent and control the bacterial disease problem faced in the high-incidence period of Nocardiosis in summer.