Application of Nocardia rubra cell wall skeleton as neutrophil modulator
A technology of Nocardia rubrum and neutrophils, which is applied in the fields of medicine, microbiology, and biopharmaceuticals, and can solve problems such as the role of neutrophils in the cell wall skeleton of Nocardia rubrum, which have not been reported.
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Embodiment 1
[0082] Example 1. Commercially available Nocardia rubrum cell wall skeleton
[0083] Nocardia erythraea cell wall skeleton (Nr-CWS) was purchased from Liaoning Gressite Biopharmaceutical Co., Ltd., with the national medicine approval number S20030009 (the solid content of the cell wall skeleton in each bottle should not be less than 60 μg, and the content of muramic acid should not be low. In 1.0μg, the sugar content is not less than 4.0μg; the reconstituted volume is 2.0ml).
Embodiment 2
[0084] Example 2. Preparation of Nocardia rubrum cell wall skeleton
[0085] The preparation method of the cell wall skeleton of S20030009 is not significantly different from the following steps, but it is adjusted due to different production scales.
[0086] 1. The cells are cultured according to a known method and collected. The cells are disrupted (eg, sonication or high pressure homogenizer disruption). It is also allowed to disrupt the cells by any suitable method known in the art. Check the broken situation under the microscope, no more than 5 visible bacteria in each field of view, and if several (10 to 30) fields of view meet this standard, it is qualified.
[0087] 2. Nucleic acid removal: centrifuge the crushed supernatant, add DNase and RNase to the obtained precipitate, and remove nucleic acid according to the operation recommended by the enzyme supplier.
[0088] 3. Removal of protein: common protease (eg trypsin) is added to the precipitate, and the protein is...
Embodiment 3
[0092] Example 3. Exemplary Preparation of Neutrophil Modulating Agents
[0093] 1. The product obtained in Example 2 (active ingredient 60 μg to 120 μg, such as 60 μg, 70 μg, 80 μg, 90 μg, 100 μg, 110 μg, 120 μg) or the commercially available product of Example 1 is coated on a dressing (such as sterile gauze), Prepared as external medicine.
[0094] 2. The product obtained in Example 2 (active ingredient 60 μg) was made into lyophilized powder.
[0095] 3. The preparation method of lotion known in the art can also be used, for example: the lotion is mostly made of water and alcohol as the dispersion medium; it is made from active ingredients, electrolytes, isotonicity regulators, etc. in the dispersion medium.
[0096] 4. The product obtained in Example 2 (active ingredient 60 μg to 120 μg, such as 60 μg, 70 μg, 80 μg, 90 μg, 100 μg, 110 μg, 120 μg) or the commercial product of Example 1 was prepared into enteric-coated capsules.
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