Application of Nocardia rubra cell wall skeleton as neutrophil modulator

A technology of Nocardia rubrum and neutrophils, which is applied in the fields of medicine, microbiology, and biopharmaceuticals, and can solve problems such as the role of neutrophils in the cell wall skeleton of Nocardia rubrum, which have not been reported.

Pending Publication Date: 2022-07-08
LIAONING GREATEST BIO-PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the role of the cell wall skeleton of Nocardia rubrum in regulating neutrophils and improving the local immune microenvironment of chronically infected wounds has not been reported in the prior art

Method used

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  • Application of Nocardia rubra cell wall skeleton as neutrophil modulator
  • Application of Nocardia rubra cell wall skeleton as neutrophil modulator
  • Application of Nocardia rubra cell wall skeleton as neutrophil modulator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1. Commercially available Nocardia rubrum cell wall skeleton

[0083] Nocardia erythraea cell wall skeleton (Nr-CWS) was purchased from Liaoning Gressite Biopharmaceutical Co., Ltd., with the national medicine approval number S20030009 (the solid content of the cell wall skeleton in each bottle should not be less than 60 μg, and the content of muramic acid should not be low. In 1.0μg, the sugar content is not less than 4.0μg; the reconstituted volume is 2.0ml).

Embodiment 2

[0084] Example 2. Preparation of Nocardia rubrum cell wall skeleton

[0085] The preparation method of the cell wall skeleton of S20030009 is not significantly different from the following steps, but it is adjusted due to different production scales.

[0086] 1. The cells are cultured according to a known method and collected. The cells are disrupted (eg, sonication or high pressure homogenizer disruption). It is also allowed to disrupt the cells by any suitable method known in the art. Check the broken situation under the microscope, no more than 5 visible bacteria in each field of view, and if several (10 to 30) fields of view meet this standard, it is qualified.

[0087] 2. Nucleic acid removal: centrifuge the crushed supernatant, add DNase and RNase to the obtained precipitate, and remove nucleic acid according to the operation recommended by the enzyme supplier.

[0088] 3. Removal of protein: common protease (eg trypsin) is added to the precipitate, and the protein is...

Embodiment 3

[0092] Example 3. Exemplary Preparation of Neutrophil Modulating Agents

[0093] 1. The product obtained in Example 2 (active ingredient 60 μg to 120 μg, such as 60 μg, 70 μg, 80 μg, 90 μg, 100 μg, 110 μg, 120 μg) or the commercially available product of Example 1 is coated on a dressing (such as sterile gauze), Prepared as external medicine.

[0094] 2. The product obtained in Example 2 (active ingredient 60 μg) was made into lyophilized powder.

[0095] 3. The preparation method of lotion known in the art can also be used, for example: the lotion is mostly made of water and alcohol as the dispersion medium; it is made from active ingredients, electrolytes, isotonicity regulators, etc. in the dispersion medium.

[0096] 4. The product obtained in Example 2 (active ingredient 60 μg to 120 μg, such as 60 μg, 70 μg, 80 μg, 90 μg, 100 μg, 110 μg, 120 μg) or the commercial product of Example 1 was prepared into enteric-coated capsules.

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Abstract

The invention relates to application of a Nocardia rubra cell wall skeleton as a neutrophil modulator. The present application provides a neutrophil modulator, which comprises a component derived from the cell wall of Nocardia rubra, and which can modulate the chemotactic function, phagocytosis function, and bactericidal function (reactive oxygen species (ROS) generation, degranulation formation) of neutrophil. The neutrophil regulator disclosed by the invention can also improve the local immune microenvironment of a chronic infection wound.

Description

[0001] This application claims the priority of the Chinese patent application (application number 202110724324X) filed on June 29, 2021. technical field [0002] The present disclosure relates to the fields of medicine, microbiology, and biopharmaceuticals. In particular, the present application relates to the use of the Nocardia rubrum cell wall skeleton as a neutrophil regulator. Background technique [0003] Nocardia rubra is a species of Nocardia. The cell wall skeleton of Nocardia rubrum can be obtained after fermentation, cell fragmentation and protease degradation of the nocardia rubra cells. [0004] In the prior art, the cell wall skeleton of Nocardia rubrum can be obtained commercially, specifically, a commodity (Nr-CWS) produced by Liaoning Grace Bio-Pharmaceutical Co., Ltd. Nocardia rubrum cell wall skeleton has been used to treat cervical erosion, cervical precancerous lesions (CN101073583A), anti-human papilloma virus (CN1935262A), skin lesions or skin ulcers...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74C12N5/0787A61P17/02A61P31/02A61P29/00A61P37/04
CPCA61K35/74C12N5/0642A61P17/02A61P31/02A61P29/00A61P37/04C12N2533/90Y02A50/30
Inventor 孙炳伟盖波张轶
Owner LIAONING GREATEST BIO-PHARM CO LTD
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