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Preparation method and application of immune gel for regulating and controlling tumor microenvironment

A tumor microenvironment and gel technology, which is applied in the field of preparation of immune gels for regulating the tumor microenvironment, can solve the problems of unsatisfactory effect of a single inhibitor and low bioavailability, achieve repair and healing, and simple preparation method , the effect that the material is easy to obtain

Pending Publication Date: 2021-09-24
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide a preparation method and application of immune gel for regulating the tumor microenvironment, which can effectively solve the problems of low bioavailability, single Problems with unsatisfactory inhibitor effects

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  • Preparation method and application of immune gel for regulating and controlling tumor microenvironment
  • Preparation method and application of immune gel for regulating and controlling tumor microenvironment
  • Preparation method and application of immune gel for regulating and controlling tumor microenvironment

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Embodiment 1

[0029] A preparation method of the immune gel for regulating the tumor microenvironment of the present invention comprises the following steps:

[0030] (1) Synthesis of Prussian blue nanoparticles doped with NO: Weigh 1.2 g of PVP-K30 powder and dissolve in 30 mM K 3 [Fe(CN) 6 ] solution, add sodium nitroprusside of equimolar mass at the same time, adjust the pH to 0 with HCl with a concentration of 12 M, stir at room temperature until the solution turns clear yellow, stir at 80°C for 12 h in the dark, and centrifuge The product was washed and centrifuged three times with the washing solvent, centrifuged at 10,000 rpm / min for 30 min, and then dried in a vacuum oven for 12 h to obtain NO-doped Prussian blue nanoparticles with a particle size of 50 nm;

[0031] The centrifugal washing solvent is one of acetone, ethanol, ultrapure water or two mixed in any ratio by volume;

[0032](2) Amination modification: Take 10 mg of NO-doped Prussian blue nanoparticles and 2 mg of an ami...

Embodiment 2

[0041] A preparation method for regulating the tumor microenvironment immune gel, comprising the following steps:

[0042] (1) Synthesis of Prussian blue nanoparticles doped with NO: Weigh 1.2 g of PVP-K30 powder and dissolve in 30 mM K 3 [Fe(CN) 6 ] solution, add equimolar mass of sodium nitroprusside at the same time, adjust the pH to 0 with HCl with a concentration of 12 M, stir at room temperature until the solution turns into a clear yellow color, stir at 80°C for 12 h in the dark, and use Ethanol, a mixture of ethanol and ultrapure water at a volume ratio of 1:1, and acetone, the product was washed and centrifuged in sequence, centrifuged at 10,000 rpm / min for 30 min, and then dried in a vacuum oven for 12 h to obtain the particle size 50 nm NO-doped Prussian blue nanoparticles;

[0043] (2) Amination modification: Take 10 mg of NO-doped Prussian blue nanoparticles and 2 mg of polypropylene amine hydrochloride with a molecular weight of 1800 in 40 mL of ultrapure water...

Embodiment 3

[0049] A preparation method for regulating the tumor microenvironment immune gel, comprising the following steps:

[0050] (1) Synthesis of Prussian blue nanoparticles doped with NO: Weigh 1.2 g of PVP-K30 powder and dissolve in 30 mM K 3 [Fe(CN) 6 ] solution, while adding equimolar mass of sodium nitroprusside, with a concentration of 12 M of HCl to adjust the pH = 0, stirring at room temperature until the solution turns into a clear yellow. Then, stir at 80°C in the dark for 12 h, wash and centrifuge the product with 2.5 times the volume of ethanol, a 1:1 mixture of ethanol and ultrapure water, and ultrapure water (10,000 rpm / min, 30 min) , placed in a vacuum drying oven at 40°C for 12 h to obtain NO-doped Prussian blue nanoparticles (ie NPNPs) with a particle size of 50 nm;

[0051] (2) Amination modification: Take 10 mg of NPs and 2 mg of polyetherimide with a molecular weight of 1800 in 40 mL of ultrapure water, stir magnetically for 24 h, and place in a dialysis bag (m...

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Abstract

The invention relates to a preparation method of immune gel for regulating and controlling a tumor microenvironment. The preparation method comprises the following steps of: (1) synthesizing NO-doped Prussian blue nanoparticles; (2) carrying out amination modification on the Prussian blue nanoparticles; (3) modifying the surfaces of the Prussian blue nanoparticles with Anti-SIRP alpha; (4) loading a CSF-1R small-molecule inhibitor BLZ945 into holes of the Prussian blue particles; (5) preparing gelling preparation and thrombin solutions; and (6) uniformly mixing the BLZ945-loaded Prussian blue nanoparticles obtained in the step (4) with the gelling preparation solution, and adding the thrombin solution to obtain the immune gel under the stimulation of thrombin. According to the invention, the preparation method is simple, materials are easy to obtain, the effect is excellent, prussian blue serves as a carrier, so that efficient loading of the medicine can be implemented; the prepared immune gel is used for treating cancer postoperative recurrence, not only continuous controlled release of local immunotherapy nanoparticles is guaranteed, but also postoperative wound repair and healing are implemented, and remarkable social and economic benefits are achieved.

Description

technical field [0001] The invention relates to the field of nano-biomedicine, in particular to a preparation method and application of immune gel for regulating tumor microenvironment. Background technique [0002] Tumors, especially malignant tumors, are common and frequently-occurring diseases that seriously endanger people's physical and mental health and life. There are many ways to treat tumors, mainly surgery and drug control. It is undeniable that for clear, localized and small tumors For solid tumors, surgical resection can achieve improved survival. However, the high recurrence rate after cancer surgery seriously affects the surgical efficacy of patients and is the main reason for treatment failure. Numerous animal and clinical test data show that postoperative recurrence and metastasis of cancer is a multi-step, multi-link, multi-factor and multi-molecular complex process involving three factors: the body, microenvironment and tumor tissue. Therefore, immunother...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K33/00A61K33/26A61K31/4439A61K39/395A61K38/48A61K47/42A61K47/36A61K47/34A61K9/52A61K47/02A61K47/32A61P35/00
CPCA61K9/06A61K33/00A61K33/26A61K31/4439A61K39/39558A61K38/4833A61K47/42A61K47/36A61K47/34A61K9/5115A61K9/5138A61K9/5146A61K9/5169A61K9/5192A61P35/00C12Y304/21005A61K2300/00
Inventor 胡玉荣刘雅斐陈丽晓刘梦康于伟黄胜楠宋成治张书豪
Owner ZHENGZHOU UNIV
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