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Nanometer enzyme-aerogel composite material, detection method of alcohol content in saliva and detection method of glucose content in blood

A composite material and aerogel technology, applied in the field of detection, can solve the problems of impact and not widely used, and achieve the effect of simple operation, low cost and high sensitivity

Active Publication Date: 2020-06-26
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The saliva alcohol test strip is a semi-quantitative detection method. The judgment of drunk driving and drunk driving is carried out through color comparison, which will affect the test results due to differences in individual color discrimination. It is not widely used at present.

Method used

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  • Nanometer enzyme-aerogel composite material, detection method of alcohol content in saliva and detection method of glucose content in blood
  • Nanometer enzyme-aerogel composite material, detection method of alcohol content in saliva and detection method of glucose content in blood
  • Nanometer enzyme-aerogel composite material, detection method of alcohol content in saliva and detection method of glucose content in blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] 1.1 Preparation of amphiphilic airgel

[0064] In polyvinyl alcohol aqueous solution 8.00g (mass concentration 6wt%), add crosslinking agent maleic acid 3.16g (the ratio of -COOH in the maleic acid and the -OH in the polyvinyl alcohol is 30 at this moment) %), then add mineral acid (concentrated sulfuric acid, 1 mL), and stir well. The reactor was placed in an oven at 200°C for 24 hours. The airgel obtained by the reaction was soaked in deionized water until the solution was neutral, and then freeze-dried.

[0065] 1.2 In situ growth of Pd@Pt nanoparticles

[0066] A solution containing Pt and Pd precursors (chloroplatinic acid, concentration 80mM and chloropalladium acid, concentration 40mM), surfactant (Pluronic F127, concentration 25mg / mL), reducing agent (ascorbic acid, concentration 200mM) was mixed with the parent The aerogels were mixed and fully infiltrated, and then reacted at 90° C. for 2 hours by a hydrothermal method to obtain a nanozyme-aerogel composite...

Embodiment 2

[0070] Detection of Alcohol Using Nanozyme-Aerogel Composite

[0071] Use the nanozyme-aerogel composite material prepared in Example 1 to collect biological samples-buffer or saliva, then add alcohol oxidase and seal. Alcohol oxidase decomposes alcohol in biological samples into hydrogen peroxide, and then under the catalysis of Pd@Pt nanoparticles in the nanozyme-airgel composite, hydrogen peroxide is decomposed into water and oxygen. Pressure detection by barometer.

[0072] Physiological conditions are used: 10 mM phosphate buffer solution, pH value is 7.4, temperature is 37°C. The alcohol contents are 0, 0.2, 0.5, 1, 2, 4, 6mM respectively. Alcohol is converted into oxygen through cascade catalysis, and the relationship between alcohol concentration and pressure is obtained, and the fitting curve conforms to ΔP=1.19C Alcohol +13.2(R 2 =0.994), the detection limit was 0.50mM.

[0073] In 40% saliva, the range of alcohol content is 0-6mM (alcohol content is 0, 0.2, 0.5...

Embodiment 3

[0079] The selectivity of this method to detect alcohol was investigated.

[0080] Alcohol analogues include methanol, acetic acid, isopropanol, glycerol, isobutanol, and the like.

[0081] Alcohol oxidase (10-40U / mg) was reacted with alcohol, methanol, acetic acid, isopropanol, glycerol, and isobutanol (both concentrations were 20mM) at 37°C for 30min, and then mixed with 1.0mg nanozyme-gas The gel composite material was reacted at 37°C for 15 minutes under airtight conditions, and the pressure was detected by a barometer.

[0082] The result is as image 3 as shown, image 3 Middle panel B is the histogram of the selective experiment for detecting alcohol.

[0083] It can be seen that compared with the above analogs, the detection of alcohol obtains the strongest response signal, and the response signals of acetic acid, isopropanol, glycerol, and isobutanol have no significant difference from the blank solution. The response signal of methanol is slightly higher because ...

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Abstract

The invention provides a nanometer enzyme-aerogel composite material. The nanometer enzyme-aerogel composite material is prepared from nanometer enzymes which are in in-situ growth in amphiphilic aerogel and have catalase activity. The nanometer enzyme-aerogel composite material provided by the invention has superduper hydrophilia, favorable mechanical properties, higher catalase activity and favorable biocompatibility. Aerogel synthesis raw materials of polyvinyl alcohol and maleic acid are reagents low in price and biologically friendly. The nanometer enzymes are in in-situ growth in the aerogel, so that stability and biological safety of nanoparticles are improved, and pollution of nanometer materials in biological application is reduced. The material can be repeatedly utilized, and after the material is recycled for ten times, the activity of the material is still as high as 84.9%. The detection limit of alcohol in a buffer solution is 0.50mM, the dynamic range for detecting the alcohol content in the saliva is 0.50-15mM, and the alcohol concentration range of driving under the influence of alcohol or other drugs and drunken driving in the national standard is covered.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a nano-enzyme-aerogel composite material and a detection method for alcohol content in saliva and glucose content in blood. Background technique [0002] The current alcohol detection methods include breath alcohol content detector (GB / T 21254-2017), headspace gas chromatography (GA / T 842-2009) and saliva alcohol test strip (GA / T 843-2009). [0003] Among them, the breath alcohol content detector is used to detect the alcohol content in the breath, the specific method is to collect the breath and detect the alcohol content by electrochemical method. The working current is not more than 120mA, and it is characterized by portable design, compact and fashionable. This method is a commonly used method for detecting drunk driving in traffic. However, this method is easily affected by the different temperature, humidity, surrounding environment and artificial blowing methods at tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/54C12Q1/26G01N7/00
CPCC12Q1/26C12Q1/54G01N7/00G01N2333/904
Inventor 杜衍张玉马崇博
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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