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Control method for biological flocculant XM-11 fermentation process grading oxygen supply

A technology of XM-11 and biological flocculants, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as analysis and in-depth systematic research, so as to reduce production energy consumption, reduce production costs, The effect of promoting the process of practical application

Inactive Publication Date: 2010-06-16
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these researchers have found that the concentration of dissolved oxygen in the fermentation system directly affects the synthesis of bioflocculants, they have not analyzed the reasons for this phenomenon, nor have they done more in-depth and systematic research on it.

Method used

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  • Control method for biological flocculant XM-11 fermentation process grading oxygen supply

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Embodiment 1

[0027] The liquid fermentation medium used included glucose 15g / L, urea 0.5g / L, and yeast extract 0.5g / L; the seed culture time was 8 hours, the inoculum size was 8%; the fermentation temperature was 25°C, and the initial pH of the medium was 4.0. A single stirring speed was maintained during the fermentation process, and the effects of different stirring speeds on the growth of bacteria were investigated. Figure 1 is the curve of the change of the bacterial volume of the bioflocculant synthetic strain with time at different stirring speeds.

[0028] As can be seen from Figure 1, there is an optimum stirring speed for the growth of bacteria——100r / min. If the stirring speed is too high or too low, the growth of the bacteria will decrease; but at a higher stirring speed, the specific growth rate of the bacteria Higher, which can shorten the time for the bacteria to reach the maximum growth. For example, when fermentation is carried out at 300r / min, the time for bacterial growth...

Embodiment 2

[0030] Using the fermentation conditions as described in Example 1, the influence of stirring speed on the growth of bacteria was investigated. The difference is that the liquid fermentation medium used contains 20g / L glucose, 1g / L urea, and 1g / L yeast extract; the seed culture time is 16 hours, the inoculum size is 15%; the fermentation temperature is 30°C, and the initial pH of the medium is 10.0.

Embodiment 3

[0032] Using the fermentation conditions as described in Example 1, the influence of stirring speed on the growth of bacteria was investigated. The difference is that in the liquid fermentation medium used, the glucose is 18g / L, the urea is 0.8g / L, and the yeast extract is 0.8g / L; the seed culture time is 12h, and the inoculum size is 10%; the fermentation temperature is 28°C, and the initial pH of the medium is 7. 8.

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Abstract

The present invention relates to biological flocculant, and is especially biological flocculant XM-11 fermenting process with controlled staged oxygen supply. Microbe Nocardia sp. XM-B11 is cultured in the fermenting culture medium through the seed culturing in the inoculation amount of 8-15 % for 8-16 hr and the liquid fermentation at 25-30 deg.c for 30-56 hr including the initial fermentation stage of 0-20 hr in stirring rotation speed of 100-150 rpm. The process can ensure the excellent growth of the spawn in the initial fermentation stage and the optimized composing state of the biologicalflocculant in the later fermentation stage, and has low power consumption and low production cost.

Description

technical field [0001] The invention relates to a biological flocculant, in particular to a method for controlling oxygen supply in stages during the fermentation process of the biological flocculant XM-11. Background technique [0002] At the end of the 1980s, Japan made remarkable achievements in the development of microbial flocculants. Kurane Ichiro and others from the Industrial Technology Research Institute of the Ministry of International Trade and Industry have isolated the S-1 strain of Rhodococcus erythroflates from the soil. And obtained the first biological flocculant-NOC-1 produced by it, which is still the best biological flocculant found so far. Kurane has carried out in-depth research on it, including the screening of its producing bacteria, optimization of fermentation conditions, separation and purification, analysis of molecular structure and application (Kurane R, Toeda K, Takeda K, Suzuki T.Culture condition for production of microbial flocculant by Rho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F1/52C12R1/365
Inventor 何宁陈坚李寅李清彪卢英华
Owner XIAMEN UNIV
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