Sulfonamide antibiotic synergistically degraded bacteria and application thereof

A technology of synergistic degradation and antibiotics, applied in the direction of bacteria, microorganisms, microorganisms, etc., to achieve the effect of broad-spectrum detoxification and degradation ability, high degradation efficiency, and great potential for bioremediation

Active Publication Date: 2020-08-11
HARBIN INSTITUTE OF TECHNOLOGY SHENZHEN (INSTITUTE OF SCIENCE AND TECHNOLOGY INNOVATION HARBIN INSTITUTE OF TECHNOLOGY SHENZHEN)
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above technical problems, the present invention discloses a sulfonamide antibiotic synergistic degradation bacterium and its application. The combination of the two strains can synergistically complete SMX (sulfamethoxazole, sulfamethoxazole) and nitrogen heterocyclic product 3-amino-5- The complete degradation of methoxazole (3A5MI) realizes sulfamethoxazole as the target pollutant, provides new ideas and new strain resources for the deep removal of sulfa antibiotics, and makes up for the current biological treatment of sulfa antibiotics. Low and stable accumulation of nitrogen heterocyclic products in the degradation process, it provides efficient degradation strain resources for the bioremediation of widely used sulfonamide antibiotics such as SMX, which has very important theoretical and application value

Method used

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  • Sulfonamide antibiotic synergistically degraded bacteria and application thereof
  • Sulfonamide antibiotic synergistically degraded bacteria and application thereof
  • Sulfonamide antibiotic synergistically degraded bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Isolation and Identification of Degrading Bacteria P27 on One Side of SMX Benzene Ring and Product Degrading Bacteria N27

[0043] 1.1 Isolation and purification of strain P27

[0044] The source of the bacteria was collected from the surplus sludge of a sewage treatment plant in Harbin. 50 mg / L of SMX was added to a 250 mL Erlenmeyer flask (containing 100 mL of sterilized inorganic salt medium) as a mother liquor, and about 5 The mud-water mixture sample of g was cultured on a constant temperature shaker at 30°C with a rotation speed of 150 rpm. After culturing for 7-8 days, prepare the medium in the same way, and transfer 10% of the volume of the enrichment solution to fresh medium. Continuously transferred several times until there were no obvious sludge particles in the enrichment solution and a uniform milky white suspension appeared, then the SMX degradation effect of the enrichment solution was measured. Using 50 mg / L SMX-inorganic salt (SMX-MM) plates, the str...

Embodiment 2

[0050] Analysis of the degradation efficiency of the degrading bacteria P27 on one side of the SMX benzene ring and the degrading bacteria N27 on nitrogen heterocyclic products

[0051] 2.1 Preparation of pure bacteria suspension

[0052] The purified strains were added to the R 2 A solid medium (peptone 0.5 g, yeast extract powder 0.5 g, casein hydrolyzate 0.5 g, glucose 0.5 g, soluble starch 0.5 g, dipotassium hydrogen phosphate 0.3 g, anhydrous magnesium sulfate 0.024 g, sodium pyruvate 0.3 g , agar 15.0 g, distilled water 1000 mL) for activation, picked a single colony to a sterilized inorganic salt medium, and added the corresponding carbon source, and cultured in a shaker at 30°C and 150 rpm. After the bacterial liquid is turbid, transfer it to fresh inorganic medium with 10% transfer amount, repeat the above operation, and the obtained pure bacterial seed liquid with stable degradation ability and growth cycle can be used for subsequent analysis of degradation efficien...

Embodiment 3

[0057] Analysis of Degradation Substrate Spectrum of Sulfa Antibiotics by Strain P27

[0058] Analysis of the substrate spectrum of strain P27: the inoculation source was the pure bacterial suspension prepared in 2.1. In this stage, it was investigated whether the strain P27 also has the ability to degrade the benzene ring side of the nine widely used sulfa antibiotics except SMX, including sulfapyridine, sulfamethazine, sulfamethizole, sulfachloropyridazine, sulfa Dimethoxine, sulfamethoxine, sulfa-5-methoxine, sulfadiazine, and sulfa-metamethoxine. In a 50 mL conical flask (containing 20 mL of sterilized inorganic salt medium), add the corresponding substrate in the form of a mother solution to a final concentration of 50 mg / L, and then add 10% transfer volume to prepare The P27 seed solution of the strain P27 was cultured at 30°C with constant temperature and shaking, and three replicates were set up for each treatment, and the culture medium without bacteria was used as t...

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Abstract

The invention provides a sulfonamide antibiotic synergistically degraded bacteria and an application thereof. The sulfonamide antibiotic synergistically degraded bacteria comprise arthrobacterium P27and nocardioides N27, wherein the arthrobacterium P27 is preserved in China General Microbiological Culture Collection Center on December 03, 2019, and the preservation number is CGMCC No. 19070; andthe nocardioides N27 is preserved in the China General Microbiological Culture Collection Center on December 03, 2019, and the preservation is CGMCC NO. 19071. According to the technical scheme, compounding of the two strains of bacteria can synergistically complete degradation of sulfamethoxazole and an azacyclo product 3-amino-5-methylisoxazole; and in addition, the strain P27 can degrade one side of benzene rings of various sulfonamide antibiotics, the degradation substrate spectrum is wide, and a new idea and a new strain resource are provided for deep removal of the sulfonamide antibiotics.

Description

technical field [0001] The invention relates to the application field of microorganisms and biodegradation thereof, in particular to a sulfonamide antibiotic synergistically degrading bacterium and its application. Background technique [0002] Sulfa antibiotics are new pollutants that are closely related to people's daily life. Their antibacterial activity mainly depends on the similarity of their structure to p-aminobenzoic acid. The two compete with dihydrofolate synthase, inhibit the synthesis of bacterial dihydrofolate, and affect the bacterial Nucleic acid synthesis, thereby preventing the growth and reproduction of bacteria. Although sulfonamide antibiotics mainly exist in trace amounts in the water environment, they can be continuously input to the environment through various channels, and compared with other drugs, sulfonamide antibiotics have slow degradation rate, strong hydrophilicity and low volatility. and other characteristics, making it present a state of "p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/38
CPCB09C1/10C02F3/341C02F2101/38C02F2101/40C12N1/205C12R2001/01
Inventor 梁斌王爱杰漆梦媛韩京龙马晓丹孔德勇
Owner HARBIN INSTITUTE OF TECHNOLOGY SHENZHEN (INSTITUTE OF SCIENCE AND TECHNOLOGY INNOVATION HARBIN INSTITUTE OF TECHNOLOGY SHENZHEN)
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